Adaptive Cerebral Neovascularization in a Model of Type 2 Diabetes
Authors
Schreihofer, Derek A.Fagan, Susan C.
Ergul, Adviye
Li, Weiguo
Prakash, Roshini
Kelley-Cobbs, Aisha I.
Ogbi, Safia
Kozak, Anna
El-Remessy, Azza B.
Issue Date
2010-012009-10-6
Metadata
Show full item recordAbstract
OBJECTIVE: The effect of diabetes on neovascularization varies between different organ systems. While excessive angiogenesis complicates diabetic retinopathy, impaired neovascularization contributes to coronary and peripheral complications of diabetes. However, how diabetes influences cerebral neovascularization is not clear. Our aim was to determine diabetes-mediated changes in the cerebrovasculature and its impact on the short-term outcome of cerebral ischemia.RESEARCH DESIGN AND METHODS: Angiogenesis (capillary density) and arteriogenesis (number of collaterals and intratree anostomoses) were determined as indexes of neovascularization in the brain of control and type 2 diabetic Goto-Kakizaki (GK) rats. The infarct volume, edema, hemorrhagic transformation, and short-term neurological outcome were assessed after permanent middleâ cerebral artery occlusion (MCAO).
RESULTS: The number of collaterals between middle and anterior cerebral arteries, the anastomoses within middleâ cerebral artery trees, the vessel density, and the level of brain-derived neurotrophic factor were increased in diabetes. Cerebrovascular permeability, matrix metalloproteinase (MMP)-9 protein level, and total MMP activity were augmented while occludin was decreased in isolated cerebrovessels of the GK group. Following permanent MCAO, infarct size was smaller, edema was greater, and there was no macroscopic hemorrhagic transformation in GK rats.
CONCLUSIONS: The augmented neovascularization in the GK model includes both angiogenesis and arteriogenesis. While adaptive arteriogenesis of the pial vessels and angiogenesis at the capillary level may contribute to smaller infarction, changes in the tight junction proteins may lead to the greater edema following cerebral ischemia in diabetes.
Citation
Diabetes. 2010 Jan 6; 59(1):228-235ae974a485f413a2113503eed53cd6c53
10.2337/db09-0902
Scopus Count
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