Shim, Su Yeon
Nguyen, Minh Dang
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AbstractFailure of axons to regenerate following acute or chronic neuronal injury is attributed to both the inhibitory glial environment and deficient intrinsic ability to re-grow. However, the underlying mechanisms of the latter remain unclear. In this study, we have investigated the role of the mammalian homologue of aspergillus nidulans NudE, Ndel1, emergently viewed as an integrator of the cytoskeleton, in axon regeneration. Ndel1 was synthesized de novo and upregulated in crushed and transected sciatic nerve axons, and, upon injury, was strongly associated with neuronal form of the intermediate filament (IF) Vimentin while dissociating from the mature neuronal IF (Neurofilament) light chain NF-L. Consistent with a role for Ndel1 in the conditioning lesion-induced neurite outgrowth of Dorsal Root Ganglion (DRG) neurons, the long lasting in vivo formation of the neuronal Ndel1/Vimentin complex was associated with robust axon regeneration. Furthermore, local silencing of Ndel1 in transected axons by siRNA severely reduced the extent of regeneration in vivo. Thus, Ndel1 promotes axonal regeneration; activating this endogenous repair mechanism may enhance neuroregeneration during acute and chronic axonal degeneration.
CitationPLoS ONE. 2008 Apr 23; 3(4):e2014
- Ndel1 controls the dynein-mediated transport of vimentin during neurite outgrowth.
- Authors: Shim SY, Samuels BA, Wang J, Neumayer G, Belzil C, Ayala R, Shi Y, Shi Y, Tsai LH, Nguyen MD
- Issue date: 2008 May 2
- Genetic study of axon regeneration with cultured adult dorsal root ganglion neurons.
- Authors: Saijilafu, Zhou FQ
- Issue date: 2012 Aug 17
- Axotomy-induced alterations in the synthesis and transport of neurofilaments and microtubules in dorsal root ganglion cells.
- Authors: Oblinger MM, Lasek RJ
- Issue date: 1988 May
- Neuronal intermediate filament protein alpha-internexin facilitates axonal neurite elongation in neuroblastoma cells.
- Authors: Shea TB, Beermann ML
- Issue date: 1999
- The return of phosphorylated and nonphosphorylated epitopes of neurofilament proteins to the regenerating optic nerve of Xenopus laevis.
- Authors: Zhao Y, Szaro BG
- Issue date: 1994 May 1
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