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    Ndel1 Promotes Axon Regeneration via Intermediate Filaments

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    Authors
    Toth, Cory
    Shim, Su Yeon
    Wang, Jian
    Jiang, Yulan
    Neumayer, Gernot
    Belzil, Camille
    Liu, Wei-Qiao
    Martinez, Jose
    Zochodne, Douglas
    Nguyen, Minh Dang
    Issue Date
    2008-04-23
    URI
    http://hdl.handle.net/10675.2/563
    
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    Abstract
    Failure of axons to regenerate following acute or chronic neuronal injury is attributed to both the inhibitory glial environment and deficient intrinsic ability to re-grow. However, the underlying mechanisms of the latter remain unclear. In this study, we have investigated the role of the mammalian homologue of aspergillus nidulans NudE, Ndel1, emergently viewed as an integrator of the cytoskeleton, in axon regeneration. Ndel1 was synthesized de novo and upregulated in crushed and transected sciatic nerve axons, and, upon injury, was strongly associated with neuronal form of the intermediate filament (IF) Vimentin while dissociating from the mature neuronal IF (Neurofilament) light chain NF-L. Consistent with a role for Ndel1 in the conditioning lesion-induced neurite outgrowth of Dorsal Root Ganglion (DRG) neurons, the long lasting in vivo formation of the neuronal Ndel1/Vimentin complex was associated with robust axon regeneration. Furthermore, local silencing of Ndel1 in transected axons by siRNA severely reduced the extent of regeneration in vivo. Thus, Ndel1 promotes axonal regeneration; activating this endogenous repair mechanism may enhance neuroregeneration during acute and chronic axonal degeneration.
    Citation
    PLoS ONE. 2008 Apr 23; 3(4):e2014
    ae974a485f413a2113503eed53cd6c53
    10.1371/journal.pone.0002014
    Scopus Count
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    Department of Neurology: Faculty Research and Presentations

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