Exocytotic Insertion of Calcium Channels Constrains Compensatory Endocytosis to Sites of Exocytosis
Authors
Smith, Robert M.Baibakov, Boris
Ikebuchi, Yoshihide
White, Benjamin H.
Lambert, Nevin A.
Kaczmarek, Leonard K.
Vogel, Steven S.
Issue Date
2000-02-21
Metadata
Show full item recordAbstract
Proteins inserted into the cell surface by exocytosis are thought to be retrieved by compensatory endocytosis, suggesting that retrieval requires granule proteins. In sea urchin eggs, calcium influx through P-type calcium channels is required for retrieval, and the large size of sea urchin secretory granules permits the direct observation of retrieval. Here we demonstrate that retrieval is limited to sites of prior exocytosis. We tested whether channel distribution can account for the localization of retrieval at exocytotic sites. We find that P-channels reside on secretory granules before fertilization, and are translocated to the egg surface by exocytosis. Our study provides strong evidence that the transitory insertion of P-type calcium channels in the surface membrane plays an obligatory role in the mechanism coupling exocytosis and compensatory endocytosis.Citation
J Cell Biol. 2000 Feb 21; 148(4):755-768Related articles
- Structural modifications induced by TPA (12-O-tetradecanoyl phorbol-13-acetate) in sea urchin eggs.
- Authors: Ciapa B, Crossley I, De Renzis G
- Issue date: 1988 Jul
- Direct membrane retrieval into large vesicles after exocytosis in sea urchin eggs.
- Authors: Whalley T, Terasaki M, Cho MS, Vogel SS
- Issue date: 1995 Dec
- Calcium-dependent exocytosis in an in vitro secretory granule plasma membrane preparation from sea urchin eggs and the effects of some inhibitors of cytoskeletal function.
- Authors: Whitaker MJ, Baker PF
- Issue date: 1983 Jul 22
- Exocytosis reconstituted from the sea urchin egg is unaffected by calcium pretreatment of granules and plasma membrane.
- Authors: Whalley T, Whitaker M
- Issue date: 1988 Aug
- Cortical granule exocytosis is triggered by different thresholds of calcium during fertilisation in sea urchin eggs.
- Authors: Matese JC, McClay DR
- Issue date: 1998 Feb