Show simple item record

dc.contributor.authorBlack, Owen
dc.contributor.authorWebster, Paul D.
dc.date.accessioned2012-10-26T16:26:35Z
dc.date.available2012-10-26T16:26:35Z
dc.date.issued1973-04-1en_US
dc.identifier.citationJ Cell Biol. 1973 Apr 1; 57(1):1-8en_US
dc.identifier.issn1540-8140en_US
dc.identifier.pmid4691394en_US
dc.identifier.urihttp://hdl.handle.net/10675.2/541
dc.description.abstractThe regulation of protein synthesis in the pigeon has been studied by comparing the capability of cell-free amino acid incorporating systems of membrane-bound and membrane-free polysomes prepared from fasted and fed birds. New methods were developed for isolating polysomes since techniques used for other tissues did not provide quantitative recovery of polysomal RNA. The sucrose gradient profile of polysomes from pigeon pancreas showed a predominance of trisome species. Although initiation factors are present on polysomes, it was found that polysomes in cell-free systems would not initiate protein synthesis without exogenous initiation factors. This suggested the presence of an inhibitor or regulator of protein synthesis. These studies show that fasting resulted in: (a) decreased amounts of polysomes; (b) disaggregation of polysomes to monosomes; (c) decreased capability of polysomes to synthesize nascent peptides and to initiate additional synthesis, apparently not related to concentration of initiation factors.
dc.rightsCopyright © 1973 by The Rockefeller University Pressen_US
dc.titlePROTEIN SYNTHESIS IN PANCREAS OF FASTED PIGEONSen_US
dc.typeArticleen_US
dc.identifier.pmcidPMC2108954en_US
dc.contributor.corporatenameDepartment of Medicine
dc.contributor.corporatenameDepartment of Cellular Biology and Anatomy
refterms.dateFOA2019-04-09T21:01:41Z
html.description.abstractThe regulation of protein synthesis in the pigeon has been studied by comparing the capability of cell-free amino acid incorporating systems of membrane-bound and membrane-free polysomes prepared from fasted and fed birds. New methods were developed for isolating polysomes since techniques used for other tissues did not provide quantitative recovery of polysomal RNA. The sucrose gradient profile of polysomes from pigeon pancreas showed a predominance of trisome species. Although initiation factors are present on polysomes, it was found that polysomes in cell-free systems would not initiate protein synthesis without exogenous initiation factors. This suggested the presence of an inhibitor or regulator of protein synthesis. These studies show that fasting resulted in: (a) decreased amounts of polysomes; (b) disaggregation of polysomes to monosomes; (c) decreased capability of polysomes to synthesize nascent peptides and to initiate additional synthesis, apparently not related to concentration of initiation factors.


Files in this item

Thumbnail
Name:
1.pdf
Size:
484.0Kb
Format:
PDF

This item appears in the following Collection(s)

Show simple item record