BENZPYRENE HYDROXYLASE ACTIVITY IN ISOLATED PARENCHYMAL AND NONPARENCHYMAL CELLS OF RAT LIVER
AbstractPrevious studies have implicated the reticuloendothelial cells of the liver in certain aspects of steroid metabolism. The similarity in the metabolism of steroids and polycyclic hydrocarbons suggested that the nonparenchymal cells possibly play a role in these areas . The present study presents evidence that at least one of the microsomal NADPH-requirig enzymes, benzpyrene hydroxylase, is present in nonparenchymal cells and, furthermore, is "inducible ." In adult rats treated with 3-methylcholanthrene or ß-naphthoflavone, the nonparenchymal cells exhibited increases in benzpyrene hydroxylase activity of 17-fold and five-fold, respectively . Treatment with phenobarbital resulted in only a slight increase in enzyme activity . Enzyme activity in parenchymal cells under similar conditions was increased sixfold and fivefold by 3-methylcholanthrene and ß-naphthoflavone, respectively, but not by phenobarbital.
CitationJ Cell Biol. 1972 Feb 1; 52(2):316-321
- Species differences in the induction of microsomal hemoproteins and 3,4-benzpyrene hydroxylase by phenobarbital and 3-methylcholanthrene.
- Authors: Alvares AP, Schilling G, Levin W
- Issue date: 1970 Oct
- Preferential induction of aryl hydroxylase activity in rat liver nuclear envelope by 3-methylcholanthrene.
- Authors: Khandwala AS, Kasper CB
- Issue date: 1973 Oct 15
- [Selective induction of a liver microsomal mixed-function oxygenase system. II. Kinetic study of benz(a)pyrene hydroxylase].
- Authors: Roberfroid M, Cumps J, Razzouk C
- Issue date: 1974 Feb
- Effects of inducers of liver microsomal enzymes on the composition of microsomal hemoprotein.
- Authors: Kuntzman R, Alvares AP, Levin W
- Issue date: 1971 May
- Microsomal hydroxylase induction in liver cell culture by phenobarbital, polycyclic hydrocarbons, and p,p'-DDT.
- Authors: Gielen JE, Nebert DW
- Issue date: 1971 Apr 9