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dc.contributor.authorMaddox, Dennis M
dc.contributor.authorCondie, Brian G.
dc.date.accessioned2010-09-24T21:26:46Z
dc.date.available2010-09-24T21:26:46Z
dc.date.issued2003-10-29en_US
dc.identifier.citationBMC Dev Biol. 2001 Jan 8; 1:1en_US
dc.identifier.issn1471-213Xen_US
dc.identifier.pmid11178105en_US
dc.identifier.urihttp://hdl.handle.net/10675.2/46
dc.description.abstractBACKGROUND: Glutamate decarboxylase (GAD) is the biosynthetic enzyme for the neurotransmitter gamma-aminobutyric acid (GABA). Mouse embryos lacking the 67-kDa isoform of GAD (encoded by the Gad1 gene) develop a complete cleft of the secondary palate. This phenotype suggests that this gene may be involved in the normal development of tissues outside of the CNS. Although Gad1 expression in adult non-CNS tissues has been noted previously, no systematic analysis of its embryonic expression outside of the nervous system has been performed. The objective of this study was to define additional structures outside of the central nervous system that express Gad1, indicating those structures that may require its function for normal development. RESULTS: Our analysis detected the localized expression of Gad1 transcripts in several developing tissues in the mouse embryo from E9.0-E14.5. Tissues expressing Gad1 included the tail bud mesenchyme, the pharyngeal pouches and arches, the ectodermal placodes of the developing vibrissae, and the apical ectodermal ridge (AER), mesenchyme and ectoderm of the limb buds. CONCLUSIONS: Some of the sites of Gad1 expression are tissues that emit signals required for patterning and differentiation (AER, vibrissal placodes). Other sites correspond to proliferating stem cell populations that give rise to multiple differentiated tissues (tail bud mesenchyme, pharyngeal endoderm and mesenchyme). The dynamic expression of Gad1 in such tissues suggests a wider role for GABA signaling in development than was previously appreciated.
dc.rightsThe PMC Open Access Subset is a relatively small part of the total collection of articles in PMC. Articles in the PMC Open Access Subset are still protected by copyright, but are made available under a Creative Commons or similar license that generally allows more liberal redistribution and reuse than a traditional copyrighted work. Please refer to the license statement in each article for specific terms of use. The license terms are not identical for all articles in this subset.en_US
dc.subject.meshAnimalsen_US
dc.subject.meshBranchial Region / embryology / enzymologyen_US
dc.subject.meshEctoderm / enzymologyen_US
dc.subject.meshEmbryo, Mammalian / enzymologyen_US
dc.subject.meshFemaleen_US
dc.subject.meshGene Expression Regulation, Developmental / geneticsen_US
dc.subject.meshGlutamate Decarboxylase / deficiency / geneticsen_US
dc.subject.meshIsoenzymes / deficiency / geneticsen_US
dc.subject.meshLimb Buds / embryology / enzymologyen_US
dc.subject.meshMesoderm / enzymologyen_US
dc.subject.meshMiceen_US
dc.subject.meshNerve Tissue / embryology / enzymologyen_US
dc.subject.meshPregnancyen_US
dc.subject.meshRNA, Messenger / geneticsen_US
dc.subject.meshTail / embryology / enzymologyen_US
dc.titleDynamic expression of a glutamate decarboxylase gene in multiple non-neural tissues during mouse development.en_US
dc.typeJournal Articleen_US
dc.typeResearch Support, Non-U.S. Gov'ten_US
dc.identifier.pmcidPMC31335en_US
dc.contributor.corporatenameInstitute of Molecular Medicine and Geneticsen_US
dc.contributor.corporatenameDepartment of Cellular Biology and Anatomyen_US
refterms.dateFOA2019-04-09T21:00:04Z
html.description.abstractBACKGROUND: Glutamate decarboxylase (GAD) is the biosynthetic enzyme for the neurotransmitter gamma-aminobutyric acid (GABA). Mouse embryos lacking the 67-kDa isoform of GAD (encoded by the Gad1 gene) develop a complete cleft of the secondary palate. This phenotype suggests that this gene may be involved in the normal development of tissues outside of the CNS. Although Gad1 expression in adult non-CNS tissues has been noted previously, no systematic analysis of its embryonic expression outside of the nervous system has been performed. The objective of this study was to define additional structures outside of the central nervous system that express Gad1, indicating those structures that may require its function for normal development. RESULTS: Our analysis detected the localized expression of Gad1 transcripts in several developing tissues in the mouse embryo from E9.0-E14.5. Tissues expressing Gad1 included the tail bud mesenchyme, the pharyngeal pouches and arches, the ectodermal placodes of the developing vibrissae, and the apical ectodermal ridge (AER), mesenchyme and ectoderm of the limb buds. CONCLUSIONS: Some of the sites of Gad1 expression are tissues that emit signals required for patterning and differentiation (AER, vibrissal placodes). Other sites correspond to proliferating stem cell populations that give rise to multiple differentiated tissues (tail bud mesenchyme, pharyngeal endoderm and mesenchyme). The dynamic expression of Gad1 in such tissues suggests a wider role for GABA signaling in development than was previously appreciated.


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