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dc.contributor.authorJessen, Jason R.
dc.date.accessioned2015-03-12T02:01:23Zen
dc.date.available2015-03-12T02:01:23Zen
dc.date.issued1999-11en
dc.identifier.urihttp://hdl.handle.net/10675.2/346505
dc.description.abstractDespite the essential roles played by the recombination activating genes (ragl and rag2) during V(D)J recombination, the mechanisms that restrict their expression to lymphoid cells are undefined. Using a novel approach to achieve artificial chromosome transgenesis in zebrafish, we demonstrate that distal regulatory elements are critical to suppress ragl expression in inappropriate tissues. In contrast to smaller reporter gene constructs, 125 and 75 kb artificial chromosomes containing the zebrafish rag genomic locus directed GFP expression in a pattern reflective of endogenous rag 1. Mapping experiments identified a positive element 5' of ragl that enhances GFP expression in both lymphoid and non-lymphoid tissues and a negative element 5' of ra g l that specifically suppresses GFP expression in the skeletal muscle. Our transgenic zebrafish also express GFP in olfactory neurons which we show represent an authentic ra g l expression site in zebrafish.
dc.relation.urlhttp://ezproxy.augusta.edu/login?url=http://search.proquest.com/docview/304539157?accountid=12365en
dc.rightsCopyright protected. Unauthorized reproduction or use beyond the exceptions granted by the Fair Use clause of U.S. Copyright law may violate federal law.en
dc.subjectZebrafishen
dc.subjectChien
dc.subjectArtificial chromosomeen
dc.subjectTransgenesisen
dc.subjectrag1 regulationen
dc.titleArtificial Chromosome Transgenesis Reveals Long-Distance Negative Regulation of ragl in Zebrafishen
dc.typeDissertationen
dc.contributor.departmentDepartment of Biochemistry and Molecular Biologyen
dc.description.advisorLin, Shuoen
dc.description.degreeDoctor of Philosophy (Ph.D.)en
dc.description.committeeHoward, Eugene; Dynan, William; Sadofsky, Moshe; Conway, Simonen
html.description.abstractDespite the essential roles played by the recombination activating genes (ragl and rag2) during V(D)J recombination, the mechanisms that restrict their expression to lymphoid cells are undefined. Using a novel approach to achieve artificial chromosome transgenesis in zebrafish, we demonstrate that distal regulatory elements are critical to suppress ragl expression in inappropriate tissues. In contrast to smaller reporter gene constructs, 125 and 75 kb artificial chromosomes containing the zebrafish rag genomic locus directed GFP expression in a pattern reflective of endogenous rag 1. Mapping experiments identified a positive element 5' of ragl that enhances GFP expression in both lymphoid and non-lymphoid tissues and a negative element 5' of ra g l that specifically suppresses GFP expression in the skeletal muscle. Our transgenic zebrafish also express GFP in olfactory neurons which we show represent an authentic ra g l expression site in zebrafish.


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