• Effects of monoamine uptake inhibitors in an assay of pain-related depression of behavior in male mice

      Alexander, Khadijah; College of Science and Mathematics; MIller, Laurence (2015-10-09)
      Consequences of pain include stimulation of some behaviors (e.g. vocalization, reflexive withdrawal from stimuli), and depression of others (e.g. exercise, and work). Pain-related decreases in behavior are among the primary diagnostic and treatment concerns for physicians, but preclinical research has often ignored this important endpoint. This discrepancy between basic research and clinical application may be one obstacle to the development of new pain treatments. In the present study, we modeled pain-related depression of behavior by examining nesting behavior in male ICR mice. Nest building is an innate mouse behavior that is sensitive to depression by a pain stimulus, and pain-related depression of nesting is blocked by the clinically effective nonsteroidal anti-inflammatory drug (NSAID) ketoprofen. This project examines effects of monoamine uptake inhibitors with varying selectivity for serotonin (5HT), norepinephrine (NE) and dopamine (DA) on pain-related depression of nesting. Citalopram (5HT-selective), nisoxetine (NE-selective), milnacipran (mixed action, 5HT/NE-selective), and bupropion (DA-selective) were evaluated for their ability to block pain-related depression of nesting. Results show that the monoamine uptake inhibitors lacking significant dopamine had no effect on pain-depressed nesting. This finding is consistent with previous work suggesting that dopamine may be a key neurochemical target in the treatment of pain-related depression of behavior. Begin Time: 09:28 End Time: 28:00
    • Histology of the Normal Mouse Trachea

      Latremouille, Rachel; Weinberger, Paul M.; College of Science and Mathematics (2015-02-06)
      The first tissue engineered tracheal transplantation was performed in 2008 and has since been proposed as a treatment for patients who suffer from severe tracheal stenosis. While the procedure has been performed experimentally on several patients in Europe, the outcomes are poorly documented and complications including patient deaths have resulted in a call for more pre-clinical investigations. Due to multiple factors, our laboratory chose to adapt a mouse model for bronchiolitis obliterans, to model tissue-engineered tracheal transplantation. Early on, it was realized that there was little literature on normal murine tracheobronchial anatomy. To resolve this problem, we set out to create a histological atlas of the murine trachea. Histologic images of normal murine tracheae (hematoxylin and eosin, and Masson's trichrome) were obtained using brightfield multispectral imaging at 5x, 1 Ox, and 1 OOx. Images were reviewed and annotated by specialists in airway surgery,veterinary medicine, and anatomic pathology. It is our hope that establishing this atlas will provide important normative data for researchers already using this model , and will cultivate increased interest in the murine model for future tracheal transplantation research. Begin Time: 09:12 End Time: 25:40