• A study of the aminopeptidases of neisseria catarrhalis

      Folds, James Donald; Department of Cell and Molecular Biology (1967-05)
    • A study of the effect of thyroxine on conn. -5 strain of coxsackie virus infection in adult mice

      Ungar, Ralph Frank; Department of Cell and Molecular Biology (1957)
    • Study of the effects of various lighting conditions on anxiety and depression in patients in a coronary care unit

      Ballard, Nancy Medley; School of Nursing (Augusta University, 1993-06)
      The purpose of this study was to evaluate the effect of three different lighting environments on the patients in a Coronary Care Unit (CCU). The sample consisted of 45 subjects with the diagnosis of myocardial infarction (MI), angina, or rule out MI. Three different groups (n=lS each group) were utilized to evaluate rooms with naturally lighted windows (NLW), artificially lighted windows with day/night sequencing (ALW), and artificial windows with n.o artificial back-lighting (AW). Room assignment was made by admissions clerks, who were not aware of the study, in the normal way. Patients who met the study criteria and gave informed consent were asked to complete the Hospital Anxiety Depression Scale (Zigmond & Snaith, 1983) after a stay of approximately 48 hrs. Results of the study did not demonstrate significant difference in anxiety and depression between the three groups. Anecdotally, three subjects in the AW group developed confusion and had to be dropped from the study. This supports earlier research by Wilson (1972) finding increased rates of confusion among patients assigned to windowless rooms.
    • Study of the effects of vitamin A deficiency in albino rats

      Hazlett, James Cummins; Department of Anatomy (1968-06)
    • A study of the relationship between perceived social support, satisfaction with social support networks, and self-rated health in older adults

      Seagraves, Pat C.; School of Nursing (Augusta University, 1992-04)
      The purpose of this study was to examine the relationship between perceived social support, satisfaction with social support networks and self-rated health in older adults. The study used a cross-sectional correlational design to examine the hypothesis that perceived social support and satisfaction with social support networks would be positively correlated (p < .05} with scores on a selfrated health measure. The convenience sample consisted of forty-three subjects ranging in age from sixty-five to ninety-five years, with a mean age of 75.4 years. All subjects were able to speak and understand English, and were judged to have the physical ability to complete a written questionnaire packet. All subjects completed Pfeiffer's Short Portable Mental Status Questionnaire (SPMSQ} with no more than two adjusted errors, indicating intact mental functioning. The Personal Resource Questionnaire (PRQ-85} was used to measure perceived social support, and investigator developed measures were used to assess satisfaction with social support and self-rated health. A list of health problems common to older adults provided an index of actual health status. Pearson correlation coefficients were calculated for satisfaction with social support networks, age, self-rated health and the subscales of PRQ-85, Part II. While the hypothesis of the study was not supported, a significant inverse correlation was demonstrated between age and nurturance, indicating that as a person ages, the opportunity for nurturant behavior decreases. The results of the data analysis reflected the multiplicity and chronicity of health problems in this age group, but revealed that older people do not evaluate their own health according to the number or type of health problems they experience. Nor is their self-rated health score consistent · with the number and type of medications they take. Further, the data from this sample woula indicate that self-rated health in this age group is not dependent on one's perception of social support, nor their satisfaction with their so"cial support network.
    • A study of the relationship between staff nurse job satisfaction and satisfaction with head nurse communication

      Bevill, James W. Jr.; School of Nursing (1990-04)
      The purpose of this study was to examine the · . relationship between staff nurse job satisfaction and staff nurse satisfaction with head nurse communication. Head n~rse communication was broken down into five dimensions: (a) communication with immediate supervisor, (b) personal. feedback, (c) media quality, (d) communication climate, and (e) organizational integration. A conceptual model based on findings from several studies was constructed for the study. Using a d~scriptive-correlational design, data obtained from 45 staff nurses employed at a south-eastern, academic university hospital was analyzed using Pearson Product Moment correlation. Price and Mueller's Measurement of General Job Satisfaction and a modified version of Downs and Hazenrs Communication Satisfaction Questionnaire were the tools used to collect the data. Findings showed a positive relationship between staff nurse job satisfactio~ and satisfaction with each of the five dimensions of head nurse communication •. The strongest relationship was found to be between staff nurse job satisfaction and satisfaction with communication from immediate supervis.or, significant at the p(0.001 level. The relationships between staff nurse job satisfaction and the remaining four dimensions of communication satisfaction were significant at the p(0.05 level.
    • A Study of the Relationship Between the Availability of Condiments and Nutritional Intake of Elderly Nursing Home Residents

      Wach, Caroline B; School of Nursing (Medical College of Georgia, 1991-01)
      The effect of the availability of condiments on the nutritional intake of 10 elderly nursing home residents was evaluated over a five day periods in which 100 meal time observations were recorded. Base line data were gathered on each subject for a prescribed number of days and then an assortment of condiments was made available to each resident for sue as he or she desired. The hypothesis was that nursing home residents to whom condiments were made available would increase their nutritional intake defined as quantity of food consumed and expressed in grams. Results however did not support this hypothesis as there was no treatment effect demonstrated ·between groups relative to lunch or dinner when considered both independent of one another and together.
    • Study of Transport Systems for Oligopeptides

      Chothe, Paresh P.; Department of Biochemistry and Molecular Biology (2010-12)
    • A study of vitamin E deficient rabbit blood

      Ellison, Arthur C.; Department of Anatomy (1963-06)
    • A study to determine the relationship between body image and success- ful weight loss

      Lindecker, Katheryn; School of Nursing (1984-02)
      The purpose of this study was to.determine. the relationship be.tween successful weight loss or unsuccessful. weight loss and body image. Thirty-.three _obese males and females aged 25-70 years were given the Secord a·nd Jourard Body Cathexis Scale at a weight reduction clinic. Subjects were divided into fo.ur groups for comparison. These groups were: those who were beginning the weight loss, those who were· successful ·in weigh.t loss, those who failed to lose .weight,- and those who failed t~ maintain weight loss. The .last· two groups were combined·- for ana lysis. · Analysi~· of variance revealed no signi.ficant difference between ·body image of those who were beginning and· those who were successful. No significant difference was revealed between. body image of those who were beginning and those who were unsuccessful'. Significant difference was found in body_ image of those who were successful and those who were uns·uccessful (.E, = • 05). v
    • Subcellular localization of collagen prolyl and lysyl hydroxylation

      Guzman, Noberto A.; Department of Cell and Molecular Biology (1975-08)
    • Submit characterization of some human and deer hemoglobins

      Plese, Charles F.; School of Allied Health Science (1972-06)
    • Super enhancer-associated molecular signatures reveal a dependency on immune and metabolic mechanisms in chronic lymphatic leukemia

      Shull, Austin Y.; Department of Biochemistry and Molecular Biology (7/12/2016)
      Chronic lymphocytic leukemia (CLL), characterized by the progressive and uncontrolled accumulation of CD19+ B cells, currently remains as an incurable malignancy despite recent advancements in treatment options. The difficulties of eliciting curative measures in CLL are partly driven by the adaptability of the transcriptional response in CLL cells. In this study, we sought to better understand the complexities of the CLL transcriptional profile by defining the large histone H3 lysine-27 acetylation regions known as “super enhancers” within B cells and determining which genes overexpressed in CLL overlapped with super enhancers. From this analysis, we identified 190 super enhancer-associated genes overexpressed in CLL and determined that many of the genes identified were either involved in immune signaling cascades (e.g. LCK, FCER2) or metabolic regulation (e.g. LSR, ENO2). These processes corresponded with our reverse phase protein array (RPPA) profile of CLL patients, which shows overexpression of immune signaling kinases (e.g. LCK) as well as alteration of metabolically sensitive translation regulators (e.g. 4E-BP1 phosphorylation). Additionally, we determined that CLL cells are apoptotically sensitive to dual PI3K/mTOR inhibition when compared to upstream B cell receptor pathway inhibition due to their differential effects on 4E-BP1 phosphorylation. Based on the derived information from our super enhancer expression signature, we then compared the effects of preferentially targeting super enhancers with either the BET bromodomain inhibitor JQ1 or the cyclin dependent kinase-7 (CDK7) inhibitor THZ1. From this comparison, we saw that JQ1 could inhibit cell cycle progression in CLL cell lines as well as differentially disrupt transcription of genes involved in immune signaling. Contrastingly, we saw that THZ1 elicited a different response in CLL cells by inducing apoptosis and differentially downregulating genes involved in metabolism. The specific super enhancer-associated genes disrupted by the respective treatments further highlighted the dichotomy of JQ1 and THZ1-mediated effects, as JQ1 suppressed the B cell activation marker gene FCER2 whereas THZ1 suppressed the glycolytic enolase gene ENO2. Collectively, these results reveal that super enhancers play a role in mediating both immune signaling and metabolic expression signatures in CLL and that super enhancers can be differentially disrupted by BET bromodomain or CDK7 inhibition.
    • Suppression of GATA-1 gene expression by a hammerhead ribozyme reduces hemoglobin synthesis in HEL cells

      Hu, Huaiyu; Department of Biochemistry and Biology (Augusta University, 1992-12)
      GATA-1 is a major erythroid trans-acting factor. To determine if GATA-1 is required for globin gene expression in HEL cells, we have used an antisense RNNribozyme strategy to suppress the expression of the gata-1. DNA coding for a hammerhead ribozyme with flanking sequences antisense to the GATA-1 mRNA was cloned into an eukaryotic expression vector pMAMneo-luc at a position within the 3'-untranslated region of the firefly luciferase gene. Following stable transfection into HEL cells, both luciferase assays and RNA-PCR were used to show that the luciferase mRNA is present. HEL cells possessing the antisense RNNribozyme showed morphological changes that may resemble macrophages. Latex uptake experiments showed that these cells indeed acquired more phagocytic activities than the_ controls suggesting that they resemble a macrophage phenotype. Northern blot analyses show a 50% reduction of GATA-1 mRNA in the HEL cells possessing the antisense RNNribozyme. Following induction with o-ALA, isoelectric focusing gel data showed a 50-90% reduction of fetal and embryonic hemoglobins in these cells. Cell growth was unaffected. Northern blot analyses also showed that the level of the chimeric luciferase mRNA was lower than that required for a stoichiometric antisense effect. Therefore, the hammerhead ribozyme may be acting catalytically. Our conclusion is that GATA-1 is requi_red for hemoglobin gene expression in HEL cells.