• Schizophrenia Susceptibility Gene TMEM108 Regulates Excitatory Synapse Development and Transmission in the Dentate Gyrus

      Bates, Ryan; Department of Neuroscience and Regenerative Medicine (2014-06)
      The primary purpose of this project is to understand the function of schizophrenia susceptibility gene Transmembrane Protein 108 (TMEM108) in the brain. First, we set out to understand the temporal and spatial expression of TMEME108 in the brain using RT-PCR, mice harboring the LacZ gene driven by the TMEM108 promoter and antibodies targeting the TMEM108 protein. Second, to address protein function mice containing a null mutation in the TMEM108 gene were generated. This mouse was used to determine the effect of TMEM108 deletion on the development of gross brain morphology. Third, we sought to understand the function of TMEM108 in synaptic development and physiology by examining the number and type of synapse as well as recording from brain slices lacking TMEM108. Fourth, we sought to understand the consequence of TMEM108 deletion on animal behavior.
    • Screening for Circadian Rhythm of Core Body Temperature in Spinal Cord Injured Patients

      Secrest, Janet A.; Department of Physiological and Technological Nursing (1987-10)
      The purpose of this study was to describe the pattern of core body temperature in spinal cord injured patients. The research question was: Is there a circadian rhythm for core body temperature in spinal cord injured patients? Subjects included 19 in patients with traumatic spinal cord transections at or above the fourth thoracic segment. Oral temperatures were monitored every four hours for a 48-hour period using an electronic thermometer. The range of individual subjects temperature ranges were 1.1 to 5.2 degrees F. The group pattern of mean temperatures from Day 1 was similar to that of Day 2.A significant difference was found between the time periods (p = .038). The higher temperatures occurred in the evening, and the lower temperatures in the morning. The finding of a circadian rhythm for core body temperature was unexpected in spinal cord injured subjects.
    • A search for [alpha] and [gamma] globin gene anomalies among SS and SC patient

      Ryan, Robrt Frederick; Department of Cell and Molecular Biology (1987-12)

      O'Neil, Rachelle; Department of Social Sciences (Augusta University, 2020-05)
      Voting for public office is often touted as a right, and the ability to do so without interference is vital to the democratic process of the United States. However, during the U.S. 2016 Presidential Election, Russia interfered with the voting process. Given that Russia and the U.S. has a long tenuous relationship that consist of a mutual back and forth, this paper proposes that the security dilemma theory offers an explanation outlining Russia’s cyberaggression toward the U.S. 2016 Presidential Election and whether the aggression posturing was offensive, defensive, or an exchange of both. Additionally, this paper conducts a literature review of the security dilemma theory and the rise of the cybersecurity dilemmas its derivative and ascertains their applicability to the proposed thesis. The paper further argues that in relation to the U.S., Russia, as a Great Power, more likely favors cyberaggression when threatened, real or perceived. This paper uses case analysis as the methodology for testing its research question and answering its thesis. The case analysis comprises of examples of the security dilemma theory, aggression, the cybersecurity dilemma , and cyberaggression exchanged between Russia and the U.S. during the Cold War and 21st Century. After reviewing analysis trends, a discussion follows that covers gapsin this research; advanced knowledge about the theory; the thesis astested; the development or testing of the theory; methodology of cases analyzed; the sources used; measurement of variables; limitations of the study; generalization of results; and finally the reliability or replicability of the results.
    • Segmental responses of the dog paw vasculature

      Hammond, Mary Corinne; Department of Physiology (1968-06)
    • Selected correlates of academic success in baccalaureate nursing students

      Lee, Janet Hughes; School of Nursing (1987-05)
      The purpose of this study~as to examine the relationship between selected cognitive and noncoghitive variables and the baccalatireate nursing student's grade point average (GPA) to determirie which variables correlate with academic success for black· and for white students. The cognitive variables were the Scholastic Aptitude Test. (SAT) scores aqd the GPA on all courses prerequisite to the nursing sequence. The noncognitive variables were social support, self-esteem and expectancy for success. All junior and senior nursing students in a baccalaureat~ nursing program located in the Southeastern U. S. were invited to participate. Data were collected on 72 students using the Norbeck Social Sripport Questionnaire, the Rosenberg Self-Esteem Scale, the Hal~-Fibel Generalized Expectancy for Success Scale, and a demographic data sheet. Entry GPA, SAT scores, and nursing GPA were obtained from the students' records. A multivariate analysis of variance utilized to test three hypotheses concerning the difference between the black· and white student groups on the noncognitive variables showed a statisiically significant difference between the black and white student· groups when the noncognitive variables were con~idered ·as a set~ However, there were no significant differences when the noncognitive variables were considered individually. Six hypotheses were tested using Pearson product-moment correlation coefficients to determine relationships between the nonq,o~gnitive variables and nursing ~\t . GPA. There were no significant relationships between the noncognitive variables and nursing GPA in the black student group. There were significant .relationships between selfesteem and nursing GPA (E = .3468), and between expectancy for success and nursing GPA (E = .2259) in the white student group. Stepwise multiple regression was used to·determine the variables accounting for significant portions of the variance in nursing GPA in the total sample (R2 = .4227). Entry GPA was the first variable to eriter into the model followed by mother's education and by self-esteem.

      Okashah, Najeah; Department of Pharmacology and Toxicology (Augusta University, 2020-03)
      Hundreds of human G protein-coupled receptors (GPCRs) converge on activation of four families of heterotrimeric G proteins. Individual receptors select a subset of G proteins in order to produce appropriate cellular responses. While the precise mechanisms of coupling selectivity are uncertain, the G alpha subunit carboxy (C) terminus is believed to be the primary region recognized by GPCRs. We directly assessed coupling between 14 representative GPCRs and 16 G alpha subunits, including one wild-type G alpha subunit from each of the four families and 12 chimeras with exchanged C termini. We found that Gi-coupled receptors were relatively selective for Gi1 heterotrimers, while Gs-, Gq-, and G12- coupled receptors were more promiscuous and always coupled in some measure to Gi1 heterotrimers. Our tests with G alpha subunit chimeras show that the G alpha subunit core and C terminus both play important roles in selectivity. This suggests that the key G protein determinants of selectivity vary widely, even for different receptors that couple to the same G protein. While promiscuous GPCR-G protein coupling is often observed. These interactions behave as expected with receptor-G protein coupling and activation being almost synonymous. Agonist bound GPCRs activate the G protein heterotrimers they interact with, while ignoring G protein subtypes that they cannot activate. However, we have shown that GPCRs can form unproductive complexes with G12 heterotrimers. Vasopressin 2 receptor (V2R) forms agonist-dependent complexes with G12 heterotrimers. Unlike V2R complexes with cognate Gs heterotrimers, V2R-G12 complexes do not dissociate when GDP or GTP is present. Stimulating V2R with arginine vasopressin (AVP) does not activate signaling responses downstream of G12 activation. Evaluation of several G12-coupled receptors demonstrated that agonist induced GPCR-G12 complexes have a wide range resistance to GDP. Like V2R receptors, formyl peptide 2 receptors (FPR2) and smoothened receptors (SMOR) formed complexes with G12 heterotrimers that were relatively resistant to GDP. Our results indicate that several GPCRs can form agonist-dependent unproductive complexes with G12 heterotrimers that are relatively resistant to GDP. Suggesting that for some GPCRs agonist-dependent association with G12 heterotrimers is weakly coupled to nucleotide exchange
    • Self-care and Cultural Meanings of Mothering in African American Women with HIV/AIDS

      Shambley-Ebron, Donna; Department of Physiological and Technological Nursing (2003-11)
      African American women as a marginalized group in American society daily face obstacles related to race, gender, and culture. The stigma of HIV/AIDS compounds the problems and issues that African American women face as they ' manage their illness, childrearing, and other responsibilities of daily living. Within the last twenty years, HIV/AIDS has undergone a transformation, with a shift in the population most affected by HIV/AIDS. Women of color and their children have become the most rapidly growing group of people living with HIV/AIDS. This study will explore how core cultural values influence the self care activities and personal meaning of mothering among African American women who are HIV- positive. An Africana Womanist framework in conjunction with critical social theory will be used to guide and analyze this study. Knowledge generated from this research study will lead to the development of culturally appropriate theory development, and has the potential for developing empowering and liberating resistance patterns in young African American women.
    • Self-care and cultural meanings of mothering in african american women with hiv/aids.

      Shambley-Ebron, Donna; School of Graduate Studies (2003-11)
      African American women who are HIV-positive and responsible for mothering an HIV-positive child face many challenges in caring for themselves and their children. This study used critical ethnographic methods to explore the experiences, values, traditions ·and beliefs of African American women to understand how these factors influence self-care and mothering. An Africana Womanist framework was used to guide this study. The study was conducted with a purposive sample of 10. African American · women from the Southeastern United States. The sample participants were HIV-positive and had at least one child who also was HIV-po~itive. The overarching theme derived from the research was "Creating a Life of Meaning", which encompassed domains of · "Disabling Relationships, "Strong Mothering", and "Redefining Self-Care". This study revealed the culturally. specific ways in which African American women mother their children and manage their own care when. living with HIV, a chronic, stigmatizing illness. This study dispels negative stereotypes of African American women and highlights the sttengt}ls ·that women use in their daily lives. This research has the potential to create an impact on the ways in which nursing care is delivered. and self-care promoted for this population.
    • Self-Perceived and Actual Competence of Registered Nurses' Evaluations of Electronic Fetal Monitoring Strips

      Bishop, Kitty Pickard; School of Nursing (1983-05)
      The·. relationship between self-perceived and. actual competence . . . . . of 16 registered nurses' evaluations of electronic- fetal monitoring ' . ' - . strips was 'exainfned 1n a 12-bed labor' an'd delive-ry u~it 1n a 7ob~bed' general hospi ~al·.in ·east .dentra 1 . G~o rg.ta. · The :nurses were admi nistered three structur~d inStruments:· The Demographic Data Questi ·a~nai re, Se·l f-Assessment of El ectro·n.i.c Fetal· Monitoring Ski 11 s, and . . ~ . . . . ~ . . ' - . •" . . . ' . . . Electronic· Fetal ·.~onitciring Ski 11 s Asse.ssment. Data were ·analyzed using Pearson 's· product moment. corre·l ati on· coefficient, and _one-way . · analyses: 'of variance .. The: findings showed no. significant relati.o·nsh.ip between-overall l • • : • se.l f:...pe·rcei ved and actua 1 competenc·e among labor and delivery room nurses' eva·l uations of .EFM strips ( r = -0 .2·295, .E_> .05). There was no· s i gni fi ca~t re 1 at ionsh·i p between n.urses •_· 6vera 11 . self-·percei ved · ·. -~ompetence-when. eva:l.uating EFM.stri'ps :and (1) 1ength . o.femployment . - ' .- ' . - . experienc:e fn.·labor·and delivery_settings, .and (2') elapsed.time since electronic fetal monitoring educq.t·ion .. A significant relationship·· did exist between self-perceived-competence in dist.inguishing be: t~een ~ar1y, va·rtab1e,··and··late 'decelerations 'a,nd. (1) exp_erien.ce. in' ·._·labor and delivery ~.ettings·, and (2) elapsed time sinceelectronic. feta 1 m6ni tori ng educat i·on.
    • Self-Regulation Intervention by Telephone to Reduce Weight and Blood Pressure in Overweight Women with Elevated Blood Pressure

      Fluker, Janet G; Department of Physiological and Technological Nursing (2005-05)
      Excess weight and increases in blood pressure are two biological risk factors that can be modified with changes in lifestyle behaviors. Lifestyle modification involving nutrition and activity level remain the cornerstone of prevention and treatment for individuals who are overweight and hypertensive. Self-regulation intervention delivered by telephone is designed to assist individuals in adopting lifestyle modifications to lose weight and reduce blood pressure. The purpose of this randomized clinical study was to test the effectiveness o f a telephone-delivered self-regulation intervention in reducing weight and systolic blood pressure in overweight women with elevated blood pressure. Secondary aims were to test the effectiveness of self-regulation intervention on weight self-efficacy, exercise selfefficacy and health status. Sixty-two overweight women with elevated blood pressure were randomly assigned to either five weeks of telephone-delivered self-regulation intervention (n=31) or usual care control group (n=31). Repeated measurements for outcome variables occurred at Baseline, 6-weeks and 10-weeks. Two-way ANOVA with one repeated factor demonstrated a significant interaction for weight x group (F= 8.79, df = 1/60 p = .004), with individuals in the self-regulation intervention group having a significantly greater weight loss as compared to individuals in the usual care group. Examining weight self-efficacy x group differences there was a significant difference in weight self-efficacy (F= 12.39, df = 1/60 p = .001) with individuals in the self-regulation intervention having a greater increase as compared to individuals in the usual care control group. There was a significant main effect for systolic blood pressure (F = 9.00, df 2/120, p < 01) and health status (F= .4.94, d f = 1/60, p = .03). There was no significant interaction for systolic blood pressure x group, exercise self-efficacy x group, or health status x group. These results support the use of telephone-delivered self-regulation intervention in the primary care setting as a more effective means than usual care to assist overweight women with elevated blood pressure in losing weight and increasing weight self-efficacy. Self-regulation was shown to be effective in lowering blood pressure and improving health status but it did not prove to be more effective than usual care.
    • Sense of Coherence and Psychological Well-Being Among Female Adult Children of Alcoholics

      Kito, Noriko; Department of Physiological and Technological Nursing (1998-11)
      Adult Children of Alcoholics (ACOA) have been identified as an at-risk population for alcoholism and depression. However, findings of previous studies have been inconsistent. Adapting the Salutogenic model proposed by Antonovsky (1979, 1987), this cross-sectional correlational study was designed to test a model of psychological well-being among female ACOAs. The constructs in the model were: past life experiences (family functioning in the family of origin and the gender of parental alcoholism), present life experiences (social functioning and self-help group attendance), generalized resistant resources (income and education), stressors (negative life events), Sense of Coherence, and psychological well-being (depression and inclination to problematic drinking behaviors). Following approval from the human assurance committee, a combination of local and Internet announcements were used to recruit self-identified ACOA women between the ages of 30 and 50, asking them to participate in an anonymous survey by mail. To measure research variables, seven pre-existing questionnaires were used with a selfdeveloped demographic questionnaire for this study. One hundred twenty-one participants returned their questionnaire, and 112 cases were used for analyses. Through path analyses, the results of testing the study hypotheses partially supported the theoretical model. Social functioning and family functioning in the family of origin significantly accounted for Sense of Coherence while Sense of Coherence showed a significant direct effect on depression. However, stressors did not significantly contribute to Sense of Coherence as originally posited. Generalized resistant resources did not show an indirect effect on Sense of Coherence. In the test of an alternative model, Sense of Coherence appeared to mediate the relationship between social functioning and depression. Also, in the alternative model, social functioning mediated between stressors and depression and between family functioning in the family of origin and depression. The study findings suggest emphasis on social functioning and Sense of Coherence in efforts to decrease depression among ACOA women.
    • Separation and characterization of proteins isolated from cow's snout epidermi

      Champman, Graeme V; Department of Cell and Molecular Biology (1973-05)
    • Separation and characterization of urinary mucopolysaccharides by column chromatography

      Lin, Michael; Department of Cell and Molecular Biology (1964-06)
    • The Separation and Characterization of Young and Old Erythrocytes

      Boyd, Evalyn; Department of Cell and Molecular Biology (1967-05)
      Results of an investigation by Huisman and Meyering (1960) of Hb-Ar in young and old cells of two adults and three infants using eM-cellulose chromatography showed that the old red blood cells contained considerably more Hb-Ar than younger red cells. Similar studies by Huisman and Horton (1964) on a more limited scale and with the use of Arnberlite IRe-50 chromatography failed to demonstrate differences in the quantities of the Hb-Ar components in hernolysates of young and old cells. Because of this discrepancy it was considered of importance to extend these observations by studying the young and old red blood cells of a relatively large number of normal healthy adult individuals. The Amberlite IRe-50 chromatographic procedure was selected for this purpose because this technique was found to have the advantage over eM-cellulose chromatography of a better differentiation of the Hb-A1a, Hb-A1b, and Hb-Arc components, while the complex of Hb with -SG groups could be demonstrated as a separate component (Hb-Ard) with its own·specific chromatographic properties. In order to assure a definite fractionation of the adult red blood cells into layers of cells with different cell-age--a-new procedure was developed, which moreover, allowed us sufficient quantities of (intact) ·cell fractions for additional characterization.
    • Separation and serologic characterization of adenovirus type 7a structural components

      Miot, Maurice Robert; Department of Cell and Molecular Biology (1969-06)
    • Separation of Adenovirus Type 5 Proteins by Disc Electrophoresis

      Bedinggield, Rebecca Birchmore; Department of Cell and Molecular Biology (1968-06)