• Bioactivity and mechanism of action of resveratrol, a polyhenolic phytoalexin, in sickle cell disease

      Agyekum, Davies G.; Medical College of Georgia (Augusta University, 2013-07)
    • Physicians' perceived incentives for association with nurse practitioners in the delivery of primary care

      Adinaro, Denise; School of Nursing (Augusta University, 1980-08)
      The purpose of this study was to identify those factors which physicians , currently associated with nurse practitioners, perceive as incentives in their motivation to associate with these nurse practitioners in providing primary care. A mail survey was sent to nurse practitioners and their associate physicians throughout the State of Georgia, with two follow-up postcard mailings. Data were collected for a 4 week period and a 30.46 return was obtained. A mean ranking of the identified incentives was performed. Two major categories of incentives were identified . To increase the quality of care was considered to be most important and to expand the services offered in general was rated second as incentives for physician association with nurse practitioners.
    • Studies on calcium transport of the sarcoplasmic reticulum in skinned cardiac muscle

      Zhu, Yu; Department of Physiology and Endocrinology (Augusta University, 1990-11)
    • Angiogenesis-associated gene expression changes in surgical skin flaps of diabetic rats

      Zhou, Miao Xian (Cindy); Medical College of Georgia (Augusta University, 2010-07)
    • Aquaporin 3 in keratinocyte differentiation

      Zhan, Xiangjian; Medical College of Georgia (Augusta University, 2003-08)
      Aquaporin 3 (AQP3) is a channel that transports both water and glycerol. AQP3- null mutant mice exhibit skin defects, including impairment of water holding capacity, barrier recovery and wound healing and decreased glycerol content. We hypothesized that AQP3 is involved in the regulation ofkeratinocyte proliferation and differentiation and this regulation is mediated, at least in part, by the functional interaction between AQP3 and phospholipase D (PLD). Here we demonstrate that AQP3 expression was down-regulated at the transcriptional level and glycerol uptake was reduced when primary mouse keratinocytes were induced to differentiate. In co-transfection experiments, we found that AQP3 decreased the promoter activity of keratin 5, a keratinocyte proliferation marker, but increased the promoter activity of keratin 10 and involucrin, an early and intermediate keratinocyte differentiation marker respectively. These results suggest that AQP3 is a regulator of early keratinocyte differentiation. In further investigatjons to determine the sigualing function of AQP3 in regulating keratinocyte differentiation, we found using sucrose gradient centrifugation, irnmunoprecipitation analysis, confocal microscopy that AQP3 and PLD2 were colocalized in lipid rafts. In addition, we demonstrated that AQP3 could contribute to the synthesis of phosphatidylglycerol (PG) and that PLD2 was able to utilize glycerol as a substrate to synthesize PG. These data suggest that AQP3 transports glycerol for use as a physiological primary alcohol substrate for-adjacent PLD2 to generate PG. Our results, together with the reports that PG is an activator of protein kinases (PKqm and PKCe) and also contributes to protein-protein interactions in membranes, suggest that glycerol AQP3-PLD2-PG is a potential signaling pathway in regulating keratinocyte differentiation.

      Zhang, Zhiqiang; Medical College of Georgia (Augusta University, 1997-05)
    • Characterization of leydig cell development in the rat testis

      Zhai, Juan; Medical College of Georgia (Augusta University, 1996-05)
    • The effect of dentin desensitizers on the retention of full crowns cemented with a variety of luting/bonding agents

      Yim, Nantiya Harnkul; Department of Oral Biology (Augusta University, 1999-04-21)
    • Structural and functional aspects of organic cation transporters

      Wu, Xiang; Medical College of Georgia (Augusta University, 1999-07)
    • Confidence interval estimation for a binomial proportion when no successes are observed

      Wimmer, Courtney; Dias, James; Medical College of Georgia (Augusta University, 2009-04)
      Confidence interval estimation for a binomial proportion is a long debated topic, resulting in a wide range of exact and approximate methods. Many of these methods perform quite poorly when the number of observed successes in a sample of size n is zero. In this case, the main objective of the investigator is usually to obtain an upper bound, i.e., the upper limit of a onesided confidence interval. Traditional notions of expected interval length and coverage probability are not applicable in this situation. In this paper we use observed interval length and p-confidence to evaluate nine confidence interval methods for a binomial proportion. We also consider approximate sample sizes needed to achieve various upper bounds near the zero boundary. We show that many popular approximation methods perform poorly based on these criteria and conclude that the-exact method has superior performance in terms of interval length and p-confidence.
    • A Variable prenatal stress paradigm as a valid drug discovery platform for cognitive deficits associatied with neuropsychiatric disorders

      Wilson, Christina Ann; Medical College of Georgia (Augusta University, 2012-10)
      Cognitive dysfunction is now recognized to be central to the functional disability of several neuropsychiatric disorders. However, treatment options for the management of cognitive symptoms are limited and the development of novel therapeutics has been made difficult by the lack of appropriate animal models. It has been suggested that variable prenatal stress (PNS) in rodents might be an etiologically appropriate model for some components of schizophrenia. Thus, the overall goal of this dissertation project was to conduct a comprehensive behavioral study of the model to assess face validity, and to make a preliminary assessment of its construct and predictive validity. Our results indicate that exposure to PNS results in elevated corticosterone levels following exposure to acute stress, increased aggressive behaviors, as well as increased locomotor activity and stereotypic behaviors. Further, PNS rats had altered innate fear responses to predator odor as well as impaired fear extinction. Additionally, PNS in rats was associated with impairments of sustained attention, inhibitory response control, and recognition memory all of which could be attenuated by the norepinephrine reuptake inhibitor, atomoxetine. Collectivity, these data ,support the premise that PNS in rodents is a valid model system for studying some behavioral components of neuropsychiatric disorders as well as their treatment.
    • Interaction of ET-1 with vasoactive mediators

      WIlliams, Jan Michael; Medical College of Georgia (Augusta University, 2005-07)
    • Expression of connexin 43 in orthodontic tooth movement in a rat model

      Whitehead, James D., III; Department of Oral Biology (Augusta University, 1998-12)
    • Relationship between job satisfaction and intent to stay

      West, Myrion J.; School of Nursing (Augusta University, 1993-01)
    • Effect of structured patient education on level of hope in cancer patient

      Wells, Gayle; School of Nursing (Augusta University, 1991-02)
      The purpose of this study was to determine the effect of patient participation in a structured education program on the level of hope in cancer patients. The sample consisted of 34 adult subjects diagnosed with cancer within the last five years. The control group (n = 17) consisted of patients receiving care at a private physician's office. The treatment group (n = 17) consisted of participants in a structured education program (I Can Cope) at three sites in the southeastern United States. The Nowotny Hope Scale (NHS) (Nowotny, 1989) was used to measure.hope. A quasiexperimental non-equivalent control group pretest posttest design was utilized tb test the following hypothesis: Adult cancer patients who attend structured educational classes will score higher on a scale measuring hope than those patients who have not attended such a class. Both groups represent naturally occurring collectives and randomization was not possible. The groups were matched by type of cancer diagnosis. The analysis of covariance (ANCOVA) revealed no significant difference (p = .139) in the adjusted posttest scores of the two groups; therefore, the hypothesis was not supported. In this study, the level of hope for participants in a structured education program did not differ from the level of a similar group not attending such a program. However, in view of the attention given to the concept of hope as a factor in facing the diagnosis of cancer it is important that research efforts continue to be directed to the discovery of effective interventions to foster hope.
    • Mathematical modeling of the human dental arch and its usefulness in longitudinal analysis of treatment effects

      Weddle, Larry; Department of Oral Biology (Augusta University, 1999-11)
      In the practice of orthodontics, the shape of the dental arches is important in the planning and implementation of treatment. Many mathematical functions have been proposed for the characterization of arch form including catenary, p~lynomials, beta, conic sections, and cubic splines. The purpose of this study was to use linear and nonlinear least squares estimation to fit polynomial, catenary-like and beta-like curves to a longitudinal dataset and evaluate both the curve fits and the longitudinal information obtained. A longitudinal dataset was obtained from a private orthodontist. Dental casts of the upper and lower arches were made at three time points for each of 20 subjects: before treatment, immediately following treatment, and following a post-retention follow-up period of at least two years. Each cast and a calibration strip was scanned into a separate image computer file. Image analysis software was used to mark the (x,y) coordinates of buccal landmarks on each tooth from first molar to first molar. The (x, y) coordinates from each cast were collected into a central database for analysis. It was desired to use least squares for curve fitting due to its wide availability and well known properties. In order to use least squares, the casts were required to have consistent x-axis and y-axis orientation. This was done by orienting the x-axis parallel to the line connecting the centroids of the posterior teeth on the right and left sides of each cast. Eight functions were used in the curves fitting. The linear least squares method was used to fit polynomials of 2nd, 3rd, 4th, and 5th order. The nonlinear least squares method was used to fit a generalized 5-parameter beta function and generalized inverse catenary functions with 3, 4, and 5 parameters. Each of the eight functions was fit to each of the 120 dental casts in the study. Curve fits were examined for each function and each subject, arch, and time point. The 4th and 5th order polynomials, the generalized beta, and the 4-parameter and 5-parameter generalized inverse catenary functions fit well. For the 4th and 5th order polynomials, the R2 values ranged from xxx to xxx with acceptable visual fits. For the nonlinear models, the model sum of squares approximated the total sum of squares and the curves yielded good visual fits to the data points. Longitudinal analysis was done using Euclidean distance as the metric in the parameter space of each model. In order to assess the parameter metric in terms of physical measurements, the Euclidean distances in the parameter spaces were correlated with intercanine width, intermolar width, and molar-incisor distance. Consistent correlations were not identified though the curve fits were excellent. A comparison of arch form change between upper and lower arches was also done. Since the upper arches changed more, checking the ability of the parameter metrics of the various models to detect the change was of interest. and 3rd order polynomials, All of the models except 2nd and molar-incisor distance measures were capable of detecting the difference in change between the upper and lower arches (ANOVA p-values ~ 0.05). In summary, this study shows a successful method of orienting the casts for curve fitting by least squares. The models with at least 4 parameters generally fit well across the range of dental casts studied with the 5- parameter models slightly superior. The longitudinal analysis indicates that traditional linear measurements such as intercanine width may not adequately measure the multidimensional aspects of arch form change. The parameter space metrics were able to discriminate between upper and lower arch form changes.