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INVESTIGATING THE REQUIREMENT OF HOB1 ON THE SENSITIVITY OF�SCHIZOSACCHAROMYCES POMBE�AFTER EXPOSURE TO VARIOUS DNA DAMAGING AGENTSQureshi, Arman; Department of Biological Sciences; Abdulovic-Cui, Amy L; Augusta University (2019-02-13)It is inherently important that when damaged, DNA is repaired efficiently and with high accuracy. BIN1 encodes a protein that plays a role in genomic stability, specifically in cell cycle regulation, chromatin remodeling, and DNA repair. Previous research has shown that the protein Bin1 exhibits an inhibitory role in the double strand break repair pathway of non-homologous end joining (NHEJ). The homolog of�BIN1,�HOB1, is found in the fission yeast,�Schizosaccharomyces pombe. To understand the role�HOB1�has on yeast survival after damage, two strains of�S. pombe, a wild type strain (WT) and a strain without�HOB1�(hob1?), were exposed to various DNA damaging agents. Each treatment introduced different types of DNA damage that require repair by different DNA repair pathways. These treatments included UV radiation, hydrogen peroxide treatment, Bleomycin treatment, and Cisplatin Treatment. After treatment with each respective agent, the death response of each strain was calculated and the % of surviving cells at multiple doses was graphed logarithmically. The data collected overwhelming support the idea that the presence of�HOB1�has a positive role on the survival of yeast after DNA damage. The WT strains tested survived better than the�hob1?�counterparts.
SURVEYING MOSSES FOR FUNGICIDAL ACTIVITYYan, Stephanie; Department of Biological Sciences; Abdulovic-Cui, Amy L; Christy, Charlotte; Augusta University (2019-02-13)The emergence of resistance to current fungicides is of serious concern because of the widespread diseases caused by fungi. One approach to this problem is to discover new compounds that have antifungal properties. Plants are extensively attacked by fungi and have evolved many defenses. These include fungicides and other defenses, such as a waxy cuticle, that make attack difficult. The mosses (Bryophyta) lack a cuticle. This makes them a likely group to survey for fungicidal activity because they may have additional chemical defenses. In this study, both aqueous and ethanolic extracts were made from crushed mosses and tested for their effect on growth of the yeast�Saccharomyces cerevisiae. �Mosses were collected across a broad geographical range (Georgia, Arkansas, and Alaska) to test the hypothesis that resistance to fungal attack may be higher in mosses adapted to warm and moist environments. Results include the demonstration of fungicidal activity in some, but not most, of the mosses. There was no correlation with geographical origin.� Both solvents seem able to extract compounds that will suppress yeast growth. In addition, we show that fungicidal properties may be lost during drying.� Several mosses showed strong enough antifungal activity that further investigation seems warranted.