• Login
    View Item 
    •   Home
    • Centers & Institutes
    • Center for Biotechnology and Genomic Medicine
    • Center for Biotechnology and Genomic Medicine: Faculty Research and Presentations
    • View Item
    •   Home
    • Centers & Institutes
    • Center for Biotechnology and Genomic Medicine
    • Center for Biotechnology and Genomic Medicine: Faculty Research and Presentations
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

    All of Scholarly CommonsCommunitiesTitleAuthorsIssue DateSubmit DateSubjectsThis CollectionTitleAuthorsIssue DateSubmit DateSubjects

    My Account

    LoginRegister

    About

    AboutCreative CommonsAugusta University LibrariesUSG Copyright Policy

    Statistics

    Display statistics

    Physical interaction and functional coupling between ACDP4 and the intracellular ion chaperone COX11, an implication of the role of ACDP4 in essential metal ion transport and homeostasis.

    • CSV
    • RefMan
    • EndNote
    • BibTex
    • RefWorks
    Thumbnail
    Name:
    1744-8069-1-15.pdf
    Size:
    599.9Kb
    Format:
    PDF
    Download
    Authors
    Guo, Dehuang
    Ling, Jennifer X
    Wang, Mong-Heng
    She, Jin-Xiong
    Gu, Jianguo
    Wang, Cong-Yi
    Issue Date
    2008-01-16
    URI
    http://hdl.handle.net/10675.2/22
    
    Metadata
    Show full item record
    Abstract
    Divalent metal ions such as copper, manganese, and cobalt are essential for cell development, differentiation, function and survival. These essential metal ions are delivered into intracellular domains as cofactors for enzymes involved in neuropeptide and neurotransmitter synthesis, superoxide metabolism, and other biological functions in a target specific fashion. Altering the homeostasis of these essential metal ions is known to connect to a number of human diseases including Alzheimer disease, amyotrophic lateral sclerosis, and pain. It remains unclear how these essential metal ions are delivered to intracellular targets in mammalian cells. Here we report that rat spinal cord dorsal horn neurons express ACDP4, a member of Ancient Conserved Domain Protein family. By screening a pretransformed human fetal brain cDNA library in a yeast two-hybrid system, we have identified that ACDP4 specifically interacts with COX11, an intracellular metal ion chaperone. Ectopic expression of ACDP4 in HEK293 cells resulted in enhanced toxicity to metal ions including copper, manganese, and cobalt. The metal ion toxicity became more pronounced when ACDP4 and COX11 were co-expressed ectopically in HEK293 cells, suggesting a functional coupling between them. Our results indicate a role of ACDP4 in metal ion homeostasis and toxicity. This is the first report revealing a functional aspect of this ancient conserved domain protein family. We propose that ACDP is a family of transporter protein or chaperone proteins for delivering essential metal ions in different mammalian tissues. The expression of ACDP4 on spinal cord dorsal horn neurons may have implications in sensory neuron functions under physiological and pathological conditions.
    Citation
    Mol Pain. 2005 Apr 19; 1:15
    ae974a485f413a2113503eed53cd6c53
    10.1186/1744-8069-1-15
    Scopus Count
    Collections
    Center for Biotechnology and Genomic Medicine: Faculty Research and Presentations

    entitlement

    Related articles

    • Manganese toxicity and Saccharomyces cerevisiae Mam3p, a member of the ACDP (ancient conserved domain protein) family.
    • Authors: Yang M, Jensen LT, Gardner AJ, Culotta VC
    • Issue date: 2005 Mar 15
    • Molecular cloning and characterization of the mouse Acdp gene family.
    • Authors: Wang CY, Yang P, Shi JD, Purohit S, Guo D, An H, Gu JG, Ling J, Dong Z, She JX
    • Issue date: 2004 Jan 15
    • Structure and metal ion binding of the first transmembrane domain of DMT1.
    • Authors: Wang D, Song Y, Li J, Wang C, Li F
    • Issue date: 2011 Jun
    • Metal ion transporters and homeostasis.
    • Authors: Nelson N
    • Issue date: 1999 Aug 16
    • The neuropeptide tyrosine Y1R is expressed in interneurons and projection neurons in the dorsal horn and area X of the rat spinal cord.
    • Authors: Brumovsky P, Hofstetter C, Olson L, Ohning G, Villar M, Hökfelt T
    • Issue date: 2006
    DSpace software (copyright © 2002 - 2023)  DuraSpace
    Quick Guide | Contact Us
    Open Repository is a service operated by 
    Atmire NV
     

    Export search results

    The export option will allow you to export the current search results of the entered query to a file. Different formats are available for download. To export the items, click on the button corresponding with the preferred download format.

    By default, clicking on the export buttons will result in a download of the allowed maximum amount of items.

    To select a subset of the search results, click "Selective Export" button and make a selection of the items you want to export. The amount of items that can be exported at once is similarly restricted as the full export.

    After making a selection, click one of the export format buttons. The amount of items that will be exported is indicated in the bubble next to export format.