Cj0596 is a periplasmic peptidyl prolyl cis-trans isomerase involved in Campylobacter jejuni motility, invasion, and colonization.
MetadataShow full item record
AbstractBACKGROUND: Campylobacter jejuni is a gastrointestinal pathogen of humans, but part of the normal flora of poultry, and therefore grows well at the respective body temperatures of 37 degrees C and 42 degrees C. Proteomic studies on temperature regulation in C. jejuni strain 81-176 revealed the upregulation at 37 degrees C of Cj0596, a predicted periplasmic chaperone that is similar to proteins involved in outer membrane protein folding and virulence in other bacteria. RESULTS: The cj0596 gene was highly conserved in 24 strains and species of Campylobacter, implying the importance of this gene. To study the role that Cj0596 plays in C. jejuni pathogenesis, a mutant derivative of strain 81-176 was constructed in which the cj0596 gene was precisely deleted. A revertant of this mutant was isolated by restoring the gene to its original chromosomal location using streptomycin counterselection. The cj0596 mutant strain demonstrated a slightly decreased growth rate and lower final growth yield, yet was more motile and more invasive of human intestinal epithelial cells than wild-type. In either single or mixed infections, the mutant was less able to colonize mice than 81-176. The cj0596 mutant also expressed altered levels of several proteins. CONCLUSION: Mutation of cj0596 has an effect on phenotypes related to C. jejuni pathogenesis, probably due to its role in the proper folding of critical outer membrane proteins.
CitationBMC Microbiol. 2009 Aug 8; 9:160
- Mutation of PEB4 alters the outer membrane protein profile of Campylobacter jejuni.
- Authors: Rathbun KM, Thompson SA
- Issue date: 2009 Nov
- A temperature-regulated Campylobacter jejuni gluconate dehydrogenase is involved in respiration-dependent energy conservation and chicken colonization.
- Authors: Pajaniappan M, Hall JE, Cawthraw SA, Newell DG, Gaynor EC, Fields JA, Rathbun KM, Agee WA, Burns CM, Hall SJ, Kelly DJ, Thompson SA
- Issue date: 2008 Apr
- Polynucleotide phosphorylase has an impact on cell biology of Campylobacter jejuni.
- Authors: Haddad N, Tresse O, Rivoal K, Chevret D, Nonglaton Q, Burns CM, Prévost H, Cappelier JM
- Issue date: 2012
- The Campylobacter jejuni dccRS two-component system is required for optimal in vivo colonization but is dispensable for in vitro growth.
- Authors: MacKichan JK, Gaynor EC, Chang C, Cawthraw S, Newell DG, Miller JF, Falkow S
- Issue date: 2004 Dec
- The virulence factor PEB4 (Cj0596) and the periplasmic protein Cj1289 are two structurally related SurA-like chaperones in the human pathogen Campylobacter jejuni.
- Authors: Kale A, Phansopa C, Suwannachart C, Craven CJ, Rafferty JB, Kelly DJ
- Issue date: 2011 Jun 17