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    A facilitated tracking and transcription mechanism of long-range enhancer function.

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    Authors
    Zhu, Xingguo
    Ling, Jianhua
    Zhang, Ling
    Pi, Wenhu
    Wu, Min
    Tuan, Dorothy
    Issue Date
    2007-09-14
    URI
    http://hdl.handle.net/10675.2/105
    
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    Abstract
    In the human epsilon-globin gene locus, the HS2 enhancer in the Locus Control Region regulates transcription of the embryonic epsilon-globin gene located over 10 kb away. The mechanism of long-range HS2 enhancer function was not fully established. Here we show that the HS2 enhancer complex containing the enhancer DNA together with RNA polymerase II (pol II) and TBP tracks along the intervening DNA, synthesizing short, polyadenylated, intergenic RNAs to ultimately loop with the epsilon-globin promoter. Guided by this facilitated tracking and transcription mechanism, the HS2 enhancer delivers pol II and TBP to the cis-linked globin promoter to activate mRNA synthesis from the target gene. An insulator inserted in the intervening DNA between the enhancer and the promoter traps the enhancer DNA and the associated pol II and TBP at the insulator site, blocking mid-stream the facilitated tracking and transcription mechanism of the enhancer complex, thereby blocking long-range enhancer function.
    Citation
    Nucleic Acids Res. 2007 Aug 17; 35(16):5532-5544
    ae974a485f413a2113503eed53cd6c53
    10.1093/nar/gkm595
    Scopus Count
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    Department of Biochemistry and Molecular Biology: Faculty Research and Presentations

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