Microtubules growth rate alteration in human endothelial cells.

Hdl Handle:
http://hdl.handle.net/10675.2/76
Title:
Microtubules growth rate alteration in human endothelial cells.
Authors:
Alieva, Irina B; Zemskov, Evgeny A.; Kireev, Igor I; Gorshkov, Boris A; Wiseman, Dean A; Black, Stephen M.; Verin, Alexander D.
Abstract:
To understand how microtubules contribute to the dynamic reorganization of the endothelial cell (EC) cytoskeleton, we established an EC model expressing EB3-GFP, a protein that marks microtubule plus-ends. Using this model, we were able to measure microtubule growth rate at the centrosome region and near the cell periphery of a single human EC and in the EC monolayer. We demonstrate that the majority of microtubules in EC are dynamic, the growth rate of their plus-ends is highest in the internal cytoplasm, in the region of the centrosome. Growth rate of microtubule plus-ends decreases from the cell center toward the periphery. Our data suggest the existing mechanism(s) of local regulation of microtubule plus-ends growth in EC. Microtubule growth rate in the internal cytoplasm of EC in the monolayer is lower than that of single EC suggesting the regulatory effect of cell-cell contacts. Centrosomal microtubule growth rate distribution in single EC indicated the presence of two subpopulations of microtubules with "normal" (similar to those in monolayer EC) and "fast" (three times as much) growth rates. Our results indicate functional interactions between cell-cell contacts and microtubules.
Citation:
J Biomed Biotechnol. 2010 Apr 26; 2010:671536
Issue Date:
6-May-2010
URI:
http://hdl.handle.net/10675.2/76
DOI:
10.1155/2010/671536
PubMed ID:
20445745
PubMed Central ID:
PMC2860155
Type:
Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't
ISSN:
1110-7251
Appears in Collections:
Vascular Biology Center: Faculty Research and Publication

Full metadata record

DC FieldValue Language
dc.contributor.authorAlieva, Irina Ben_US
dc.contributor.authorZemskov, Evgeny A.en_US
dc.contributor.authorKireev, Igor Ien_US
dc.contributor.authorGorshkov, Boris Aen_US
dc.contributor.authorWiseman, Dean Aen_US
dc.contributor.authorBlack, Stephen M.en_US
dc.contributor.authorVerin, Alexander D.en_US
dc.date.accessioned2010-09-24T21:44:43Z-
dc.date.available2010-09-24T21:44:43Z-
dc.date.issued2010-05-06en_US
dc.identifier.citationJ Biomed Biotechnol. 2010 Apr 26; 2010:671536en_US
dc.identifier.issn1110-7251en_US
dc.identifier.pmid20445745en_US
dc.identifier.doi10.1155/2010/671536en_US
dc.identifier.urihttp://hdl.handle.net/10675.2/76-
dc.description.abstractTo understand how microtubules contribute to the dynamic reorganization of the endothelial cell (EC) cytoskeleton, we established an EC model expressing EB3-GFP, a protein that marks microtubule plus-ends. Using this model, we were able to measure microtubule growth rate at the centrosome region and near the cell periphery of a single human EC and in the EC monolayer. We demonstrate that the majority of microtubules in EC are dynamic, the growth rate of their plus-ends is highest in the internal cytoplasm, in the region of the centrosome. Growth rate of microtubule plus-ends decreases from the cell center toward the periphery. Our data suggest the existing mechanism(s) of local regulation of microtubule plus-ends growth in EC. Microtubule growth rate in the internal cytoplasm of EC in the monolayer is lower than that of single EC suggesting the regulatory effect of cell-cell contacts. Centrosomal microtubule growth rate distribution in single EC indicated the presence of two subpopulations of microtubules with "normal" (similar to those in monolayer EC) and "fast" (three times as much) growth rates. Our results indicate functional interactions between cell-cell contacts and microtubules.en_US
dc.rightsThe PMC Open Access Subset is a relatively small part of the total collection of articles in PMC. Articles in the PMC Open Access Subset are still protected by copyright, but are made available under a Creative Commons or similar license that generally allows more liberal redistribution and reuse than a traditional copyrighted work. Please refer to the license statement in each article for specific terms of use. The license terms are not identical for all articles in this subset.en_US
dc.subject.meshCell Lineen_US
dc.subject.meshCentrosome / metabolismen_US
dc.subject.meshEndothelial Cells / cytology / metabolism / physiologyen_US
dc.subject.meshGreen Fluorescent Proteins / genetics / metabolismen_US
dc.subject.meshHumansen_US
dc.subject.meshMicroscopy, Fluorescenceen_US
dc.subject.meshMicroscopy, Videoen_US
dc.subject.meshMicrotubule-Associated Proteins / genetics / metabolismen_US
dc.subject.meshMicrotubules / metabolism / physiologyen_US
dc.subject.meshModels, Biologicalen_US
dc.subject.meshRecombinant Fusion Proteins / genetics / metabolismen_US
dc.titleMicrotubules growth rate alteration in human endothelial cells.en_US
dc.typeJournal Articleen_US
dc.typeResearch Support, N.I.H., Extramuralen_US
dc.typeResearch Support, Non-U.S. Gov'ten_US
dc.identifier.pmcidPMC2860155en_US
dc.contributor.corporatenameVascular Biology Centeren_US

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