The temporal and spatial expression pattern of the LGI1 epilepsy predisposition gene during mouse embryonic cranial development

Hdl Handle:
http://hdl.handle.net/10675.2/744
Title:
The temporal and spatial expression pattern of the LGI1 epilepsy predisposition gene during mouse embryonic cranial development
Authors:
Silva, Jeane; Wang, Guanghu; Cowell, John K. ( 0000-0002-2079-5950 )
Abstract:
Background: Mutations in the LGI1 gene predispose to a rare, hereditary form of temporal epilepsy. Currently, little is known about the temporal and spatial expression pattern of Lgi1 during normal embryogenesis and so to define this more clearly we used a transgenic mouse line that expresses GFP under the control of Lgi1 cis-regulatory elements.; Results: During embryonic brain growth, high levels of Lgi1 expression were found in the surface ectoderm, the neuroepithelium, mesenchymal connective tissue, hippocampus, and sensory organs, such as eye, tongue, and the olfactory bulb. Lgi1 was also found in the cranial nerve nuclei and ganglia, such as vestibular, trigeminal, and dorsal ganglia. Expression of Lgi1 followed an orchestrated pattern during mouse development becoming more subdued in areas of the neocortex of the mid- and hind-brain in early postnatal animals, although high expression levels were retained in the choroid plexus and hippocampus. In late postnatal stages, Lgi1 expression continued to be detected in many areas in the brain including, hippocampus, paraventricular thalamic nuclei, inferior colliculus, and the cerebral aqueduct. We also showed that Lgi1-expressing cells co-express nestin, DCX, and beta-III tubulin suggesting that Lgi1-expressing cells are migratory neuroblasts.; Conclusion: These observations imply that Lgi1 may have a role in establishing normal brain architecture and neuronal functions during brain development suggesting that it may be involved in neurogenesis and neuronal plasticity, which become more specifically defined in the adult animal.
Citation:
BMC Neurosci. 2011 May 13; 12:43
Issue Date:
13-May-2011
URI:
http://hdl.handle.net/10675.2/744
DOI:
10.1186/1471-2202-12-43
PubMed ID:
21569517
PubMed Central ID:
PMC3120723
Type:
Article
ISSN:
1471-2202
Appears in Collections:
Georgia Cancer Center: Faculty Research and Presentations

Full metadata record

DC FieldValue Language
dc.contributor.authorSilva, Jeaneen_US
dc.contributor.authorWang, Guanghuen_US
dc.contributor.authorCowell, John K.en_US
dc.date.accessioned2012-10-26T20:27:55Z-
dc.date.available2012-10-26T20:27:55Z-
dc.date.issued2011-05-13en_US
dc.identifier.citationBMC Neurosci. 2011 May 13; 12:43en_US
dc.identifier.issn1471-2202en_US
dc.identifier.pmid21569517en_US
dc.identifier.doi10.1186/1471-2202-12-43en_US
dc.identifier.urihttp://hdl.handle.net/10675.2/744-
dc.description.abstractBackground: Mutations in the LGI1 gene predispose to a rare, hereditary form of temporal epilepsy. Currently, little is known about the temporal and spatial expression pattern of Lgi1 during normal embryogenesis and so to define this more clearly we used a transgenic mouse line that expresses GFP under the control of Lgi1 cis-regulatory elements.en_US
dc.description.abstractResults: During embryonic brain growth, high levels of Lgi1 expression were found in the surface ectoderm, the neuroepithelium, mesenchymal connective tissue, hippocampus, and sensory organs, such as eye, tongue, and the olfactory bulb. Lgi1 was also found in the cranial nerve nuclei and ganglia, such as vestibular, trigeminal, and dorsal ganglia. Expression of Lgi1 followed an orchestrated pattern during mouse development becoming more subdued in areas of the neocortex of the mid- and hind-brain in early postnatal animals, although high expression levels were retained in the choroid plexus and hippocampus. In late postnatal stages, Lgi1 expression continued to be detected in many areas in the brain including, hippocampus, paraventricular thalamic nuclei, inferior colliculus, and the cerebral aqueduct. We also showed that Lgi1-expressing cells co-express nestin, DCX, and beta-III tubulin suggesting that Lgi1-expressing cells are migratory neuroblasts.en_US
dc.description.abstractConclusion: These observations imply that Lgi1 may have a role in establishing normal brain architecture and neuronal functions during brain development suggesting that it may be involved in neurogenesis and neuronal plasticity, which become more specifically defined in the adult animal.en_US
dc.rightsCopyright ©2011 Silva et al; licensee BioMed Central Ltd.en_US
dc.subjectResearch Articleen_US
dc.titleThe temporal and spatial expression pattern of the LGI1 epilepsy predisposition gene during mouse embryonic cranial developmenten_US
dc.typeArticleen_US
dc.identifier.pmcidPMC3120723en_US
dc.contributor.corporatenameGHSU Cancer Center-
dc.contributor.corporatenameDepartment of Neurology-
dc.contributor.corporatenameInstitute of Molecular Medicine and Genetics-

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