Investigation of the Effects of Leptin Treatment Towards AlphaAdrenergic Receptor Expression in Mouse Aortic, Renal, and Mesenteric Arteries and Adrenal Glands.

Hdl Handle:
http://hdl.handle.net/10675.2/621762
Title:
Investigation of the Effects of Leptin Treatment Towards AlphaAdrenergic Receptor Expression in Mouse Aortic, Renal, and Mesenteric Arteries and Adrenal Glands.
Authors:
Momtahan, Mina
Abstract:
Body weight gain and obesity lead to an elevation in blood pressure (BP) also called hypertension when the level of blood pressure reaches 140 and 90 mmHg for the systolic and diastolic BP respectively. Previous work from this lab demonstrated that the adipocyte-derived hormone leptin, secreted in important amounts in obesity, reduces the ability of the aortic artery to contract in response to adrenergic stimulation, likely mediated by a decreased expression of the aorta alpha-adrenergic receptors. However, it is not known whether leptin decreases the expression of alpha-adrenergic receptors in other arteries such as the mesenteric and renal, as well as the adrenal glands that play a key role in the control of BP. To determine whether leptin decreases alpha-adrenergic receptors expression in these arteries, as it does in the aorta, we infused leptin (10ug/day) by implanting subcutaneous mini-pumps in a total of 10 male C57bl/6 mice and evaluated leptin-mediated changes via alpha-adrenergic receptor expression. Real-time PCR (qPCR) conducted on the arteries revealed fold changes which were not supportive of our hypothesis that leptin- mediated sympatho-activation decreases adrenergic receptor expression.
Affiliation:
Vascular Biology Center
Publisher:
Augusta University
Issue Date:
12-Dec-2017
URI:
http://hdl.handle.net/10675.2/621762
Type:
Thesis
Language:
en_US
Series/Report no.:
Spring 2018
Appears in Collections:
Honors Program Theses

Full metadata record

DC FieldValue Language
dc.contributor.authorMomtahan, Minaen
dc.date.accessioned2018-02-16T16:11:50Z-
dc.date.available2018-02-16T16:11:50Z-
dc.date.issued2017-12-12-
dc.identifier.urihttp://hdl.handle.net/10675.2/621762-
dc.description.abstractBody weight gain and obesity lead to an elevation in blood pressure (BP) also called hypertension when the level of blood pressure reaches 140 and 90 mmHg for the systolic and diastolic BP respectively. Previous work from this lab demonstrated that the adipocyte-derived hormone leptin, secreted in important amounts in obesity, reduces the ability of the aortic artery to contract in response to adrenergic stimulation, likely mediated by a decreased expression of the aorta alpha-adrenergic receptors. However, it is not known whether leptin decreases the expression of alpha-adrenergic receptors in other arteries such as the mesenteric and renal, as well as the adrenal glands that play a key role in the control of BP. To determine whether leptin decreases alpha-adrenergic receptors expression in these arteries, as it does in the aorta, we infused leptin (10ug/day) by implanting subcutaneous mini-pumps in a total of 10 male C57bl/6 mice and evaluated leptin-mediated changes via alpha-adrenergic receptor expression. Real-time PCR (qPCR) conducted on the arteries revealed fold changes which were not supportive of our hypothesis that leptin- mediated sympatho-activation decreases adrenergic receptor expression.en
dc.language.isoen_USen
dc.publisherAugusta Universityen
dc.relation.ispartofseriesSpring 2018en
dc.rightsCopyright protected. Unauthorized reproduction or use beyond the exceptions granted by the Fair Use clause of U.S. Copyright law may violate federal law.en
dc.titleInvestigation of the Effects of Leptin Treatment Towards AlphaAdrenergic Receptor Expression in Mouse Aortic, Renal, and Mesenteric Arteries and Adrenal Glands.en_US
dc.typeThesisen
dc.contributor.departmentVascular Biology Centeren
dc.description.advisorBelin de Chantemele, Eric Jen
All Items in Scholarly Commons are protected by copyright, with all rights reserved, unless otherwise indicated.