Hdl Handle:
http://hdl.handle.net/10675.2/621750
Title:
Isolation and Culture of Microglia
Authors:
Doughty, Deanna; Venugopal, Natasha
Abstract:
Glioblastoma (GBM) is the most aggressive and common adult brain tumor subtype, with the majority of patients surviving less than one year. The GBM microenvironment is composed of tumor cells as well as non-cancerous cells, such as microglia, a component of the immune system in the brain. To better understand the role of microglia in GBM, we have optimized in vitroculture conditions for primary microglia. Growing microglia in culture is challenging, but this technique is needed for planned future experiments. Microglia were isolated from mouse neuronal tissue by magnetic bead antibody cell separation using the cellular marker CX3CR1. Isolated microglia were then cultured in various culture conditions, and cellular morphology by light microscopy was used to determine cell health, viability, and activation status. It was determined that the primary microglia grow best in neurobasal media in wells coated with poly-D lysine. Future studies aim to isolate a larger number of cells to allow forco-culture of the inactivated microglia with GBM cells. These results will allow us to better understand the role that microglia play in GBM progression.
Affiliation:
Department of Biological Sciences
Issue Date:
12-Feb-2018
URI:
http://hdl.handle.net/10675.2/621750
Submitted date:
26-JAN-2018 03:42PM
Type:
Poster Presentation
Description:
Presentation given at the 19th Annual Phi Kappa Phi Student Research and Fine Arts Conference
Appears in Collections:
19th Annual PKP Student Research and Fine Arts Conference: Posters

Full metadata record

DC FieldValue Language
dc.contributor.authorDoughty, Deannaen
dc.contributor.authorVenugopal, Natashaen
dc.date.accessioned2018-02-12T17:19:36Z-
dc.date.available2018-02-12T17:19:36Z-
dc.date.issued2018-02-12-
dc.date.submitted26-JAN-2018 03:42PM-
dc.identifier.urihttp://hdl.handle.net/10675.2/621750-
dc.descriptionPresentation given at the 19th Annual Phi Kappa Phi Student Research and Fine Arts Conferenceen
dc.description.abstractGlioblastoma (GBM) is the most aggressive and common adult brain tumor subtype, with the majority of patients surviving less than one year. The GBM microenvironment is composed of tumor cells as well as non-cancerous cells, such as microglia, a component of the immune system in the brain. To better understand the role of microglia in GBM, we have optimized in vitroculture conditions for primary microglia. Growing microglia in culture is challenging, but this technique is needed for planned future experiments. Microglia were isolated from mouse neuronal tissue by magnetic bead antibody cell separation using the cellular marker CX3CR1. Isolated microglia were then cultured in various culture conditions, and cellular morphology by light microscopy was used to determine cell health, viability, and activation status. It was determined that the primary microglia grow best in neurobasal media in wells coated with poly-D lysine. Future studies aim to isolate a larger number of cells to allow forco-culture of the inactivated microglia with GBM cells. These results will allow us to better understand the role that microglia play in GBM progression.en
dc.subjectGlioblastomaen
dc.subjectMicrogliaen
dc.titleIsolation and Culture of Microgliaen
dc.typePoster Presentationen
dc.contributor.departmentDepartment of Biological Sciencesen
cr.funding.sourceUniversity Start-up Fundsen
dc.contributor.sponsorBradford, Jenniferen
dc.contributor.sponsorDepartment of Biological Sciencesen
dc.contributor.affiliationAugusta Universityen
All Items in Scholarly Commons are protected by copyright, with all rights reserved, unless otherwise indicated.