Hdl Handle:
http://hdl.handle.net/10675.2/621745
Title:
Epigenetic modifications in rat pancreas following ethanol abuse.
Authors:
Liao, Kristie; Nancy Jhanji; Pruitt, Allison; Patton, Tadd; Hernandez, Caterina
Abstract:
Chronic consumption of alcohol can lead to pancreatitis, which can predispose to pancreatic cancer. Because combinations of histone modifications have been implicated in pancreatic tumorigenesis, our goal is to find histone modifications in pancreatic acinar cell nuclei following by ethanol abuse. As an animal model of alcoholism, alcohol preferring (P) rats and alcohol non-preferring rats (NP) were used. Histones were extracted from rat pancreatic nuclear fractions using 0.4 N sulfuric acid and dialysis. Histone modifications were studied by Western-blotting analysis using the following antibodies: anti-trimethyl-histone H3 at Lys9 (H3K9me3), anti-dimethyl-histone H3 at Lys9, anti-phospho-histone H3 at Ser10, anti-acetyl-histone H3 atLys14, anti-histone H3, anti-acetyl-histone H4, anti-histone H4. We found that alcohol abuse caused a decrease in the phosphorylation of histone H3. This result was observed in pancreatic tumor specimens.Further studies are needed to determine the extentto which both modifications are related and which gene expression is affected.
Affiliation:
Department of Biological Sciences; College of Nursing; Department of Psychological Sciences; Department of Pharmaceutical Sciences
Issue Date:
12-Feb-2018
URI:
http://hdl.handle.net/10675.2/621745
Submitted date:
26-JAN-2018 02:12PM
Type:
Poster Presentation
Description:
Presentation given at the 19th Annual Phi Kappa Phi Student Research and Fine Arts Conference
Appears in Collections:
19th Annual PKP Student Research and Fine Arts Conference: Posters

Full metadata record

DC FieldValue Language
dc.contributor.authorLiao, Kristieen
dc.contributor.authorNancy Jhanjien
dc.contributor.authorPruitt, Allisonen
dc.contributor.authorPatton, Tadden
dc.contributor.authorHernandez, Caterinaen
dc.date.accessioned2018-02-12T17:19:35Z-
dc.date.available2018-02-12T17:19:35Z-
dc.date.issued2018-02-12-
dc.date.submitted26-JAN-2018 02:12PM-
dc.identifier.urihttp://hdl.handle.net/10675.2/621745-
dc.descriptionPresentation given at the 19th Annual Phi Kappa Phi Student Research and Fine Arts Conferenceen
dc.description.abstractChronic consumption of alcohol can lead to pancreatitis, which can predispose to pancreatic cancer. Because combinations of histone modifications have been implicated in pancreatic tumorigenesis, our goal is to find histone modifications in pancreatic acinar cell nuclei following by ethanol abuse. As an animal model of alcoholism, alcohol preferring (P) rats and alcohol non-preferring rats (NP) were used. Histones were extracted from rat pancreatic nuclear fractions using 0.4 N sulfuric acid and dialysis. Histone modifications were studied by Western-blotting analysis using the following antibodies: anti-trimethyl-histone H3 at Lys9 (H3K9me3), anti-dimethyl-histone H3 at Lys9, anti-phospho-histone H3 at Ser10, anti-acetyl-histone H3 atLys14, anti-histone H3, anti-acetyl-histone H4, anti-histone H4. We found that alcohol abuse caused a decrease in the phosphorylation of histone H3. This result was observed in pancreatic tumor specimens.Further studies are needed to determine the extentto which both modifications are related and which gene expression is affected.en
dc.subjectepigenetic modificationsen
dc.subjectalcohol abuseen
dc.subjectpancreasen
dc.titleEpigenetic modifications in rat pancreas following ethanol abuse.en
dc.typePoster Presentationen
dc.contributor.departmentDepartment of Biological Sciencesen
dc.contributor.departmentCollege of Nursingen
dc.contributor.departmentDepartment of Psychological Sciencesen
dc.contributor.departmentDepartment of Pharmaceutical Sciencesen
cr.funding.sourceTranslational Research Awarden
dc.contributor.sponsorSabbatini, Maria Eugeniaen
dc.contributor.sponsorDepartment of Biological Sciencesen
dc.contributor.affiliationAugusta Universityen
dc.contributor.affiliationAppalachian College of Pharmacyen
All Items in Scholarly Commons are protected by copyright, with all rights reserved, unless otherwise indicated.