Hdl Handle:
http://hdl.handle.net/10675.2/621733
Title:
CHARACTERIZATION OF NF-¿B DEFICIENT BONE-MARROW MACROPHAGES
Authors:
Pepper, Anthony; Fischer, Jeffrey
Abstract:
The aim of this study was tocharacterize bone-marrow derived macrophages (BMDMs) that lack canonical nuclear factor-kappaB (NF-?B) signaling. The macrophages for the study were obtained by harvesting the bone-marrow fromp65LysMCre (KO) mice and LysMCrecontrolmice.To determine NF-?B deletion efficiency, p65 (a transcription factor in the canonical pathway) protein levels were evaluated by fluorescent microscopyin bothKOand control BMDMs that had been stimulated withlipopolysaccharide(LPS).The induction ofiNOSwasmonitoredin KO and control BMDMswhenactivated by NF-?B stimulatorsIFN-?andLPS.The regulation of iNOSwas assessedby comparing macrophages that had been treated withLPS, IFN-?, or both to a control treatment under fluorescent microscopy. In addition to staining, a nitric oxide assay was employed to help determine the extent of iNOS activity.The macrophages were also visualized under light microcopyby comparing macrophagesthat were stimulated with LPS andIFN-?tounstimulated cellsusing fluorescence microscopy.Currently,a caspase assay is in progress to help further evaluate the effects of p65 losswithin macrophages.
Affiliation:
Department of Biological Sciences
Issue Date:
12-Feb-2018
URI:
http://hdl.handle.net/10675.2/621733
Submitted date:
26-JAN-2018 04:53PM
Type:
Poster Presentation
Description:
Presentation given at the 19th Annual Phi Kappa Phi Student Research and Fine Arts Conference
Appears in Collections:
19th Annual PKP Student Research and Fine Arts Conference: Posters

Full metadata record

DC FieldValue Language
dc.contributor.authorPepper, Anthonyen
dc.contributor.authorFischer, Jeffreyen
dc.date.accessioned2018-02-12T17:19:35Z-
dc.date.available2018-02-12T17:19:35Z-
dc.date.issued2018-02-12-
dc.date.submitted26-JAN-2018 04:53PM-
dc.identifier.urihttp://hdl.handle.net/10675.2/621733-
dc.descriptionPresentation given at the 19th Annual Phi Kappa Phi Student Research and Fine Arts Conferenceen
dc.description.abstractThe aim of this study was tocharacterize bone-marrow derived macrophages (BMDMs) that lack canonical nuclear factor-kappaB (NF-?B) signaling. The macrophages for the study were obtained by harvesting the bone-marrow fromp65LysMCre (KO) mice and LysMCrecontrolmice.To determine NF-?B deletion efficiency, p65 (a transcription factor in the canonical pathway) protein levels were evaluated by fluorescent microscopyin bothKOand control BMDMs that had been stimulated withlipopolysaccharide(LPS).The induction ofiNOSwasmonitoredin KO and control BMDMswhenactivated by NF-?B stimulatorsIFN-?andLPS.The regulation of iNOSwas assessedby comparing macrophages that had been treated withLPS, IFN-?, or both to a control treatment under fluorescent microscopy. In addition to staining, a nitric oxide assay was employed to help determine the extent of iNOS activity.The macrophages were also visualized under light microcopyby comparing macrophagesthat were stimulated with LPS andIFN-?tounstimulated cellsusing fluorescence microscopy.Currently,a caspase assay is in progress to help further evaluate the effects of p65 losswithin macrophages.en
dc.subjectNF-kappaBen
dc.subjectbone-marrow derived macrophageen
dc.titleCHARACTERIZATION OF NF-¿B DEFICIENT BONE-MARROW MACROPHAGESen
dc.typePoster Presentationen
dc.contributor.departmentDepartment of Biological Sciencesen
dc.contributor.sponsorBradford, Jenniferen
dc.contributor.sponsorDepartment of Biological Sciencesen
dc.contributor.affiliationAugusta Universityen
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