Investigating the Role of Hob1 In Translesion Synthesis in Schizosaccharomyces pombe

Hdl Handle:
http://hdl.handle.net/10675.2/621241
Title:
Investigating the Role of Hob1 In Translesion Synthesis in Schizosaccharomyces pombe
Authors:
Walton, Breana R.
Abstract:
If a cell should need to divide, replication of the DNA is vital. DNA can incur damage that can impede the progression of the replication process. The translesion synthesis (TLS) pathway bypasses damage allowing replication to continue. Research conducted by Sakamuro at the AU Cancer Center indicates that the protein Rev1, a crucial protein involved in the TLS pathway, physically interacts with Bin1, a protein involved in cancer progression in mammalian cells. We hypothesize that the two genes operate in the same pathway in yeast as they do in mammalian cells, and we intend to test this genetically. In our experiment we investigate whether the role of Hob1, the homolog of Bin1 in fission yeast, functions in the same pathway with Rev1 to relieve the stress of DNA damage during replication. To test this hypothesis, we obtained a hob1Δ strain and created a double mutant strain, rev1hob1Δ. To assess whether the two genes HOB1 and REV1 operate in the same pathway, a mutation assay looking for an epistatic relationship was conducted.
Affiliation:
Department of Biological Sciences
Issue Date:
May-2016
URI:
http://hdl.handle.net/10675.2/621241
Type:
Thesis
Language:
en
Series/Report no.:
Spring; 2016
Appears in Collections:
Honors Program Theses

Full metadata record

DC FieldValue Language
dc.contributor.authorWalton, Breana R.en
dc.date.accessioned2016-11-10T19:38:58Z-
dc.date.available2016-11-10T19:38:58Z-
dc.date.issued2016-05-
dc.identifier.urihttp://hdl.handle.net/10675.2/621241-
dc.description.abstractIf a cell should need to divide, replication of the DNA is vital. DNA can incur damage that can impede the progression of the replication process. The translesion synthesis (TLS) pathway bypasses damage allowing replication to continue. Research conducted by Sakamuro at the AU Cancer Center indicates that the protein Rev1, a crucial protein involved in the TLS pathway, physically interacts with Bin1, a protein involved in cancer progression in mammalian cells. We hypothesize that the two genes operate in the same pathway in yeast as they do in mammalian cells, and we intend to test this genetically. In our experiment we investigate whether the role of Hob1, the homolog of Bin1 in fission yeast, functions in the same pathway with Rev1 to relieve the stress of DNA damage during replication. To test this hypothesis, we obtained a hob1Δ strain and created a double mutant strain, rev1hob1Δ. To assess whether the two genes HOB1 and REV1 operate in the same pathway, a mutation assay looking for an epistatic relationship was conducted.en
dc.language.isoenen
dc.relation.ispartofseriesSpringen
dc.relation.ispartofseries2016en
dc.rightsCopyright protected. Unauthorized reproduction or use beyond the exceptions granted by the Fair Use clause of U.S. Copyright law may violate federal law.en
dc.subjectTranslesion synthesisen
dc.subjectYeast, Drieden
dc.subjectMutationen
dc.titleInvestigating the Role of Hob1 In Translesion Synthesis in Schizosaccharomyces pombeen
dc.typeThesisen
dc.contributor.departmentDepartment of Biological Sciencesen
dc.description.advisorAbdulovic-Cui Amyen
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