Inactivation of the WASF3 gene in prostate cancer cells leads to suppression of tumorigenicity and metastases

Hdl Handle:
http://hdl.handle.net/10675.2/605
Title:
Inactivation of the WASF3 gene in prostate cancer cells leads to suppression of tumorigenicity and metastases
Authors:
Teng, Yong; Ren, MingQiang; Cheney, Richard; Sharma, Shruti; Cowell, John K. ( 0000-0002-2079-5950 )
Abstract:
Background:: The WASF3 protein is involved in cell movement and invasion, and to investigate its role in prostate cancer progression we studied the phenotypic effects of knockdown in primary tumors and cell lines.; Methods:: ShRNA was used to knockdown WASF3 function in prostate cell lines. Cell motility (scratch wound assay), anchorage independent growth and in vivo tumorigenicity and metastasis were then compared between knockdown and wild-type cells.; Results:: Increased levels of expression were seen in high-grade human prostate cancer and in the PC3 and DU145 cell lines. Inactivation of WASF3 using shRNAs reduced cell motility and invasion in these cells and reduced anchorage independent growth in vitro. The loss of motility was accompanied by an associated increase in stress fiber formation and focal adhesions. When injected subcutaneously into severe combined immunodeficiency (SCID) mice, tumor formation was significantly reduced for PC3 and DU145 cells with WASF3 knockdown and in vivo metastasis assays using tail vain injection showed a significant reduction for PC3 and DU145 cells. The loss of the invasion phenotype was accompanied by down-regulation of matrix metalloproteinase 9.; Conclusions:: Overall, these observations demonstrate a critical role for WASF3 in the progression of prostate cancer and identify a potential target to control tumorigenicity and metastasis.
Citation:
Br J Cancer. 2010 Sep 28; 103(7):1066-1075
Issue Date:
28-Sep-2010 ; 17-Aug-2010
URI:
http://hdl.handle.net/10675.2/605
DOI:
10.1038/sj.bjc.6605850
PubMed ID:
20717117
PubMed Central ID:
PMC2965863
Type:
Article
ISSN:
1532-1827
Appears in Collections:
Georgia Cancer Center: Faculty Research and Presentations

Full metadata record

DC FieldValue Language
dc.contributor.authorTeng, Yongen_US
dc.contributor.authorRen, MingQiangen_US
dc.contributor.authorCheney, Richarden_US
dc.contributor.authorSharma, Shrutien_US
dc.contributor.authorCowell, John K.en_US
dc.date.accessioned2012-10-26T16:26:51Z-
dc.date.available2012-10-26T16:26:51Z-
dc.date.issued2010-09-28en_US
dc.date.issued2010-08-17en_US
dc.identifier.citationBr J Cancer. 2010 Sep 28; 103(7):1066-1075en_US
dc.identifier.issn1532-1827en_US
dc.identifier.pmid20717117en_US
dc.identifier.doi10.1038/sj.bjc.6605850en_US
dc.identifier.urihttp://hdl.handle.net/10675.2/605-
dc.description.abstractBackground:: The WASF3 protein is involved in cell movement and invasion, and to investigate its role in prostate cancer progression we studied the phenotypic effects of knockdown in primary tumors and cell lines.en_US
dc.description.abstractMethods:: ShRNA was used to knockdown WASF3 function in prostate cell lines. Cell motility (scratch wound assay), anchorage independent growth and in vivo tumorigenicity and metastasis were then compared between knockdown and wild-type cells.en_US
dc.description.abstractResults:: Increased levels of expression were seen in high-grade human prostate cancer and in the PC3 and DU145 cell lines. Inactivation of WASF3 using shRNAs reduced cell motility and invasion in these cells and reduced anchorage independent growth in vitro. The loss of motility was accompanied by an associated increase in stress fiber formation and focal adhesions. When injected subcutaneously into severe combined immunodeficiency (SCID) mice, tumor formation was significantly reduced for PC3 and DU145 cells with WASF3 knockdown and in vivo metastasis assays using tail vain injection showed a significant reduction for PC3 and DU145 cells. The loss of the invasion phenotype was accompanied by down-regulation of matrix metalloproteinase 9.en_US
dc.description.abstractConclusions:: Overall, these observations demonstrate a critical role for WASF3 in the progression of prostate cancer and identify a potential target to control tumorigenicity and metastasis.en_US
dc.rightsCopyright © 2010 Cancer Research UKen_US
dc.subjectMolecular Diagnosticsen_US
dc.titleInactivation of the WASF3 gene in prostate cancer cells leads to suppression of tumorigenicity and metastasesen_US
dc.typeArticleen_US
dc.identifier.pmcidPMC2965863en_US
dc.contributor.corporatenameGHSU Cancer Center-
dc.contributor.corporatenameDepartment of Pathology-

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