Hdl Handle:
http://hdl.handle.net/10675.2/602102
Title:
Epidermal Growth Factor Mediates Di-N-Octyl Phthalate-Induced Hepatocyte Proliferation
Authors:
Buckner, Shelby
Abstract:
Certain chemicals used in manufacturing plastics are linked to severe, negative health effects such as cancer. Di-N-Octylphthalate (DNOP) is a phthalate found in many plastics and has been linked with hepatocellular carcinoma. The aim of this research project was to study the effect of DNOP on the proliferation of normal mouse hepatocytes and the growth factor implicated. The expression of several growth factors and receptors (epidermal growth factor receptor (egfr), epidermal growth factor (egf), insulin-like growth factor 1 receptor (igf1r), insulin-like growth factor 1 (igf1), insulin-like growth factor 2 (igf2), and hepatocyte growth factor (hgf)) was assessed in normal hepatocytes AML-12 cell line by RT-PCR and qPCR. The rate of cell proliferation of AML-12 cells was measured using MTT cell proliferation assay kit. DNOP at 0.1% caused an increase in expression of egf at 24h, 48h, and 72 h. This result was confirmed by Western blot. DNOP did not cause changes in any of other studied genes. The rate of cell proliferation increased in those cells treated with 0.1% DNOP at 72 h and 96 h. In conclusion, our observation indicates that DNOP, through an increase in the expression of egf, acts as a proliferative agent in normal mouse hepatocytes. Funding Source: Center for Undergraduate Research and Scholarship, Department of Biological Sciences and Scholarly Activity Award
Affiliation:
Department of Biological Sciences
Issue Date:
Mar-2016
URI:
http://hdl.handle.net/10675.2/602102
Type:
Presentation
Language:
en_US
Description:
Presentation given at the 17th Annual Phi Kappa Phi Student Research and Fine Arts Conference
Appears in Collections:
17th Annual Phi Kappa Phi Student Research and Fine Arts Conference: Oral Symposia I

Full metadata record

DC FieldValue Language
dc.contributor.authorBuckner, Shelbyen
dc.date.accessioned2016-03-17T18:41:18Zen
dc.date.available2016-03-17T18:41:18Zen
dc.date.issued2016-03en
dc.identifier.urihttp://hdl.handle.net/10675.2/602102en
dc.descriptionPresentation given at the 17th Annual Phi Kappa Phi Student Research and Fine Arts Conferenceen
dc.description.abstractCertain chemicals used in manufacturing plastics are linked to severe, negative health effects such as cancer. Di-N-Octylphthalate (DNOP) is a phthalate found in many plastics and has been linked with hepatocellular carcinoma. The aim of this research project was to study the effect of DNOP on the proliferation of normal mouse hepatocytes and the growth factor implicated. The expression of several growth factors and receptors (epidermal growth factor receptor (egfr), epidermal growth factor (egf), insulin-like growth factor 1 receptor (igf1r), insulin-like growth factor 1 (igf1), insulin-like growth factor 2 (igf2), and hepatocyte growth factor (hgf)) was assessed in normal hepatocytes AML-12 cell line by RT-PCR and qPCR. The rate of cell proliferation of AML-12 cells was measured using MTT cell proliferation assay kit. DNOP at 0.1% caused an increase in expression of egf at 24h, 48h, and 72 h. This result was confirmed by Western blot. DNOP did not cause changes in any of other studied genes. The rate of cell proliferation increased in those cells treated with 0.1% DNOP at 72 h and 96 h. In conclusion, our observation indicates that DNOP, through an increase in the expression of egf, acts as a proliferative agent in normal mouse hepatocytes. Funding Source: Center for Undergraduate Research and Scholarship, Department of Biological Sciences and Scholarly Activity Awarden
dc.language.isoen_USen
dc.subjectMiceen
dc.subjectPlasticsen
dc.subjectPhthalic Acidsen
dc.subjectBlotting, Westernen
dc.titleEpidermal Growth Factor Mediates Di-N-Octyl Phthalate-Induced Hepatocyte Proliferationen_US
dc.typePresentationen
dc.contributor.departmentDepartment of Biological Sciencesen
dc.description.advisorSabbatini, Mariaen
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