Hdl Handle:
http://hdl.handle.net/10675.2/601578
Title:
A Prospective Dominant Negative Mutant of Wnt Signaling In Zebrafish Causes Craniofacial Asymmetry with Low Penetrance
Authors:
Ravilla, Dheeraj; Neiswender, Hannah
Abstract:
Wnt gene signaling pathways have been implicated in development, cell behavior, and diseases, including craniofacial abnormalities. We created mutant complementary DNA constructs using QuikChange mutagenesis and then compared them to wild-type cDNA for effects on zebrafish development following injection into one-cell embryos. We hypothesized that disrupting a putative Wnt-binding lipocalin motif would allow mutant tinagl1 mRNAs to induce a dominant negative phenotype, similar to tinagl1 gene knockdown. Mutant LCN-W2 but not WT mRNA preferentially gave small eyes and ventral body curvature similar to the gene knockdown. Our main focus was on craniofacial development using Alcian blue staining of cartilage elements in 5 day old zebrafish. Abnormalities were seen at low penetrance in high-survival (high-quality) clutches with the highest injected dose of TIN LCN-W2,150 pg. These included smaller head and asymmetric head skeleton with one smaller eye on side of variable cartilage defects. Craniofacial defects, especially asymmetry, were more prevalent in clutches with lower survival rates. These asymmetric defects had not been seen in the gene knockdown. In summary, the phenotypes of LCN-W2 partially support similarity to a dominant negative phenotype with cartilage defects, small eyes, and ventrally curved body, but the craniofacial asymmetry appears novel. More research is needed for further understanding.
Affiliation:
Department of Biological Sciences; Department of Cellular Biology and Anatomy
Issue Date:
Mar-2016
URI:
http://hdl.handle.net/10675.2/601578
Type:
Presentation
Language:
en_US
Description:
Presentation given at the 17th Annual Phi Kappa Phi Student Research and Fine Arts Conference
Appears in Collections:
17th Annual Phi Kappa Phi Student Research and Fine Arts Conference: Oral Symposia I

Full metadata record

DC FieldValue Language
dc.contributor.authorRavilla, Dheerajen
dc.contributor.authorNeiswender, Hannahen
dc.date.accessioned2016-03-17T13:11:15Zen
dc.date.available2016-03-17T13:11:15Zen
dc.date.issued2016-03en
dc.identifier.urihttp://hdl.handle.net/10675.2/601578en
dc.descriptionPresentation given at the 17th Annual Phi Kappa Phi Student Research and Fine Arts Conferenceen
dc.description.abstractWnt gene signaling pathways have been implicated in development, cell behavior, and diseases, including craniofacial abnormalities. We created mutant complementary DNA constructs using QuikChange mutagenesis and then compared them to wild-type cDNA for effects on zebrafish development following injection into one-cell embryos. We hypothesized that disrupting a putative Wnt-binding lipocalin motif would allow mutant tinagl1 mRNAs to induce a dominant negative phenotype, similar to tinagl1 gene knockdown. Mutant LCN-W2 but not WT mRNA preferentially gave small eyes and ventral body curvature similar to the gene knockdown. Our main focus was on craniofacial development using Alcian blue staining of cartilage elements in 5 day old zebrafish. Abnormalities were seen at low penetrance in high-survival (high-quality) clutches with the highest injected dose of TIN LCN-W2,150 pg. These included smaller head and asymmetric head skeleton with one smaller eye on side of variable cartilage defects. Craniofacial defects, especially asymmetry, were more prevalent in clutches with lower survival rates. These asymmetric defects had not been seen in the gene knockdown. In summary, the phenotypes of LCN-W2 partially support similarity to a dominant negative phenotype with cartilage defects, small eyes, and ventrally curved body, but the craniofacial asymmetry appears novel. More research is needed for further understanding.en
dc.language.isoen_USen
dc.subjectnt Signaling Pathwayen
dc.subjectCraniofacial Abnormalitiesen
dc.subjectDNA, Complementaryen
dc.subjectZebrafishen
dc.titleA Prospective Dominant Negative Mutant of Wnt Signaling In Zebrafish Causes Craniofacial Asymmetry with Low Penetranceen_US
dc.typePresentationen
dc.contributor.departmentDepartment of Biological Sciencesen
dc.contributor.departmentDepartment of Cellular Biology and Anatomyen
dc.description.advisorLeMosy, Ellen K.en
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