Hdl Handle:
http://hdl.handle.net/10675.2/601057
Title:
Exploring the Function of Hob1 in the Non-Homologous End Joining Repair Pathway in Schizosaccharomyces Pombe
Authors:
Ozturk, Sarah
Abstract:
Mutations in DNA induce many diseases, including cancer. The human protein, Bin1, has anticancer properties and interacts with proteins involved in maintaining DNA stability and integrity. Work completed at the AU Cancer Center has shown that the Bin1 protein is specifically involved in the inhibition of the non-homologous end-joining pathway (NHEJ), a pathway that repairs DNA breaks. However, NHEJ is mutagenic because the DNA break is restored but some nucleotides are removed. To complement this work, we are investigating the role of Hob1, the homolog of Bin1, in fission yeast, in NHEJ. If Hob1 functions in a similar manner to Bin1, then removal of Hob1 from yeast should increase the cells’ ability to repair breaks in the DNA. We are testing this hypothesis using a genetic yeast transformation protocol that measures how efficient the yeast are at converting a linear piece of DNA into a repaired circular piece of DNA. Our data from two independent experiments show that removal of Hob1 has increased the rate of NHEJ. This result supports the hypothesis that Hob1 and Bin1 have a similar role in the repair process of DNA breaks.
Affiliation:
Department of Biological Sciences
Issue Date:
Mar-2016
URI:
http://hdl.handle.net/10675.2/601057
Type:
Other
Language:
en_US
Description:
Poster presented at the 17th Annual Phi Kappa Phi Student Research and Fine Arts Conference
Appears in Collections:
17th Annual Phi Kappa Phi Student Research and Fine Arts Conference: Posters

Full metadata record

DC FieldValue Language
dc.contributor.authorOzturk, Sarahen
dc.date.accessioned2016-03-09T18:40:13Zen
dc.date.available2016-03-09T18:40:13Zen
dc.date.issued2016-03en
dc.identifier.urihttp://hdl.handle.net/10675.2/601057en
dc.descriptionPoster presented at the 17th Annual Phi Kappa Phi Student Research and Fine Arts Conferenceen
dc.description.abstractMutations in DNA induce many diseases, including cancer. The human protein, Bin1, has anticancer properties and interacts with proteins involved in maintaining DNA stability and integrity. Work completed at the AU Cancer Center has shown that the Bin1 protein is specifically involved in the inhibition of the non-homologous end-joining pathway (NHEJ), a pathway that repairs DNA breaks. However, NHEJ is mutagenic because the DNA break is restored but some nucleotides are removed. To complement this work, we are investigating the role of Hob1, the homolog of Bin1, in fission yeast, in NHEJ. If Hob1 functions in a similar manner to Bin1, then removal of Hob1 from yeast should increase the cells’ ability to repair breaks in the DNA. We are testing this hypothesis using a genetic yeast transformation protocol that measures how efficient the yeast are at converting a linear piece of DNA into a repaired circular piece of DNA. Our data from two independent experiments show that removal of Hob1 has increased the rate of NHEJ. This result supports the hypothesis that Hob1 and Bin1 have a similar role in the repair process of DNA breaks.en
dc.language.isoen_USen
dc.subjectDNAen
dc.subjectGRU Cancer Centeren
dc.subjectHoblen
dc.subjectBinlen
dc.subjectSaccharomyces cerevisiaeen
dc.subjectDNA Breaks, Double-Strandeden
dc.titleExploring the Function of Hob1 in the Non-Homologous End Joining Repair Pathway in Schizosaccharomyces Pombeen_US
dc.typeOtheren
dc.contributor.departmentDepartment of Biological Sciencesen
dc.description.advisorAbdulovic-Cui, Amy; Sakamuro, Daitokuen
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