Hdl Handle:
http://hdl.handle.net/10675.2/600968
Title:
Ac3/Ac-Associated Protein 1 Complex Regulates Actin Filament Dynamics In Pancreatic
Authors:
Newsome, Ariel
Abstract:
Adenylyl cyclase-associated protein 1 (CAP1) is a scaffolding protein involved in the regulation of actin filament formation and cell motility. CAP1 plays a role in the motility of pancreatic cancer cells. Using co-immunoprecipitation, our data showed an upregulation of adenylyl cyclase 1 and 3, and an association with CAP1 in pancreatic cancer cell lines, HPAC and PANC-1. The goal of this project was to study whether CAP1 links AC1 and AC3 to globular actin in pancreatic cancer cells. AC1 or AC3 was knocked down using small interference RNA. After stimulation with forskolin, a direct stimulator of adenylyl cyclase, HPAC and PANC-1 cells were lysed. Total lysate was rotated with anti-actin antibody followed by complex collection with an immuno- precipitation reagent. The immunocomplexes were probed with anti-CAP1 antibody in western-blotting. The results showed that in scrambled siRNA cells stimulated with forskolin, the association between CAP1 and actin was not affected. The CAP1-actin complex was impaired in AC3-deficient cells, but was not modified in AC-1 deficient cells. In conclusion, CAP1 acts as a scaffolding protein by holding the complex between G-actin and AC3, but not AC1. Funding Source: Center for Undergraduate Research & Scholarship, Department of Biological Sciences, Scholarly Activity Award
Affiliation:
Department of Biological Sciences
Issue Date:
Mar-2016
URI:
http://hdl.handle.net/10675.2/600968
Type:
Other
Language:
en_US
Description:
Poster presented at the 17th Annual Phi Kappa Phi Student Research and Fine Arts Conference
Appears in Collections:
17th Annual Phi Kappa Phi Student Research and Fine Arts Conference: Posters

Full metadata record

DC FieldValue Language
dc.contributor.authorNewsome, Arielen
dc.date.accessioned2016-03-08T20:38:19Zen
dc.date.available2016-03-08T20:38:19Zen
dc.date.issued2016-03en
dc.identifier.urihttp://hdl.handle.net/10675.2/600968en
dc.descriptionPoster presented at the 17th Annual Phi Kappa Phi Student Research and Fine Arts Conferenceen
dc.description.abstractAdenylyl cyclase-associated protein 1 (CAP1) is a scaffolding protein involved in the regulation of actin filament formation and cell motility. CAP1 plays a role in the motility of pancreatic cancer cells. Using co-immunoprecipitation, our data showed an upregulation of adenylyl cyclase 1 and 3, and an association with CAP1 in pancreatic cancer cell lines, HPAC and PANC-1. The goal of this project was to study whether CAP1 links AC1 and AC3 to globular actin in pancreatic cancer cells. AC1 or AC3 was knocked down using small interference RNA. After stimulation with forskolin, a direct stimulator of adenylyl cyclase, HPAC and PANC-1 cells were lysed. Total lysate was rotated with anti-actin antibody followed by complex collection with an immuno- precipitation reagent. The immunocomplexes were probed with anti-CAP1 antibody in western-blotting. The results showed that in scrambled siRNA cells stimulated with forskolin, the association between CAP1 and actin was not affected. The CAP1-actin complex was impaired in AC3-deficient cells, but was not modified in AC-1 deficient cells. In conclusion, CAP1 acts as a scaffolding protein by holding the complex between G-actin and AC3, but not AC1. Funding Source: Center for Undergraduate Research & Scholarship, Department of Biological Sciences, Scholarly Activity Awarden
dc.language.isoen_USen
dc.subjectAdenylyl Cyclase 1en
dc.subjectPancreatic Neoplasmsen
dc.subjectNuclear Proteinsen
dc.titleAc3/Ac-Associated Protein 1 Complex Regulates Actin Filament Dynamics In Pancreaticen_US
dc.typeOtheren
dc.contributor.departmentDepartment of Biological Sciencesen
dc.description.advisorSabbatini, Mariaen
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