The FluoroDichroSpectroPhotometer: Multi-function Instrumentation for Biophysical Spectroscopy in the Ultraviolet, Visible and Near Infrare

Hdl Handle:
http://hdl.handle.net/10675.2/594633
Title:
The FluoroDichroSpectroPhotometer: Multi-function Instrumentation for Biophysical Spectroscopy in the Ultraviolet, Visible and Near Infrare
Authors:
Sutherland, John C.
Abstract:
The decade-wide region of the electromagnetic spectrum from wavelengths of roughly 150 nm in the far ultraviolet (UV) to 1.5 μ in the near infrared (NIR) is particularly important for biophysical spectroscopy because water is relatively transparent, while most other molecules important in bio-molecular systems absorb in some part of this "water window". In addition to spectrophotometers, which measure the absorption spectrum of a sample, the most widely used instruments are fluorometers, which measure the light emitted after absorption of a photon, and dichrometers, which measure the difference between the absorption or emission of two polarizations, either circular or linear. I will describe the rationales for, and the design and construction of a laboratory instrument capable of measuring fluorescence as well as circular dichroism (CD) and magnetic CD (MCD). A second instrument of the same general type was optimized for CD in the far and vacuum UV by the use of UV radiation from a synchrotron light source. Other developments have included the simultaneous measurement of CD and the absorption spectrum of the sample, fluorescence detected CD/MCD, and the measurement of fluorescence polarization anisotropy using the components normally associated with a dichrometer. Since its introduction in 1969, essentially all dichrometers have used photoelastic modulators (PEM) to periodically modulate the polarization of a monochromatic photon beam, which makes possible the detection of very small differences in the absorption of different polarization components of the same wavelength – to about one part in a million. I will also discuss the programming of PEMs as a function of wavelength to achieve their proper operation for the measurement of CD/MCD, linear dichroism (LD), and fluorescence polarization anisotropy, and the limits of certain approximations made in the derivation of the mathematical descriptions of the operations of dichrometers.
Affiliation:
Augusta University
Issue Date:
22-Jan-2016
URI:
http://hdl.handle.net/10675.2/594633
Type:
Presentation
Language:
en_US
Description:
Presentation given at the Materials Science Research Seminar Series on Friday, January 29, 2016, 1 – 2 PM.
Series/Report no.:
Spring; 2016
Appears in Collections:
Materials Science Research Seminar Series

Full metadata record

DC FieldValue Language
dc.contributor.authorSutherland, John C.en
dc.date.accessioned2016-01-22T21:03:30Zen
dc.date.available2016-01-22T21:03:30Zen
dc.date.issued2016-01-22en
dc.identifier.urihttp://hdl.handle.net/10675.2/594633en
dc.descriptionPresentation given at the Materials Science Research Seminar Series on Friday, January 29, 2016, 1 – 2 PM.en
dc.description.abstractThe decade-wide region of the electromagnetic spectrum from wavelengths of roughly 150 nm in the far ultraviolet (UV) to 1.5 μ in the near infrared (NIR) is particularly important for biophysical spectroscopy because water is relatively transparent, while most other molecules important in bio-molecular systems absorb in some part of this "water window". In addition to spectrophotometers, which measure the absorption spectrum of a sample, the most widely used instruments are fluorometers, which measure the light emitted after absorption of a photon, and dichrometers, which measure the difference between the absorption or emission of two polarizations, either circular or linear. I will describe the rationales for, and the design and construction of a laboratory instrument capable of measuring fluorescence as well as circular dichroism (CD) and magnetic CD (MCD). A second instrument of the same general type was optimized for CD in the far and vacuum UV by the use of UV radiation from a synchrotron light source. Other developments have included the simultaneous measurement of CD and the absorption spectrum of the sample, fluorescence detected CD/MCD, and the measurement of fluorescence polarization anisotropy using the components normally associated with a dichrometer. Since its introduction in 1969, essentially all dichrometers have used photoelastic modulators (PEM) to periodically modulate the polarization of a monochromatic photon beam, which makes possible the detection of very small differences in the absorption of different polarization components of the same wavelength – to about one part in a million. I will also discuss the programming of PEMs as a function of wavelength to achieve their proper operation for the measurement of CD/MCD, linear dichroism (LD), and fluorescence polarization anisotropy, and the limits of certain approximations made in the derivation of the mathematical descriptions of the operations of dichrometers.en
dc.language.isoen_USen
dc.relation.ispartofseriesSpringen
dc.relation.ispartofseries2016en
dc.subjectWave Lengthsen
dc.subjectInstrument Developmenten
dc.subjectFluorescence Polarizationen
dc.titleThe FluoroDichroSpectroPhotometer: Multi-function Instrumentation for Biophysical Spectroscopy in the Ultraviolet, Visible and Near Infrareen_US
dc.typePresentationen
dc.contributor.departmentAugusta Universityen
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