The Effects of Acute and Repeated Exposure/s to the Organophosphate Pesticide Chlorpyrifos at Subthreshold Doses on Brain Structural Integrity

Hdl Handle:
http://hdl.handle.net/10675.2/565805
Title:
The Effects of Acute and Repeated Exposure/s to the Organophosphate Pesticide Chlorpyrifos at Subthreshold Doses on Brain Structural Integrity
Authors:
Lalani, Ashish; Murphy, Shannon; Beck, W. Dan; Poddar, Indrani; Terry, Alvin V.; Hernandez, Caterina M.
Abstract:
Organophosphates are a class of chemicals that are used in pesticides, herbicides, and also as nerve agents. Organophosphate pesticides are ubiquitous among agricultural fields and the prolonged effects of organophosphates are not well understood. It is believed that exposure to Organophosphates can cause neurological deficits and impaired neurobehavioral function. We hypothesize that the organophosphate pesticide chlorpyrifos (CPF) is associated with mechanisms that directly and/or indirectly disrupt axonal transport in brain regions integral for learning and memory. To assay this claim, rats were treated acutely with CPF at a subtoxic dose (18 mg/kg) or with repeated exposures for 14 consecutive days at subtoxic doses (3.0, 10.0 or 18 mg/kg). Whole brain was collected 6 or 24 hours after acute exposure or directly following 14 days of repeated exposure or a 30-day drug-free washout period. For my project, I focused on the prefrontal cortex, a brain region important for executive function. Prefrontal cortex was processed using a subcellular fractionation protocol and extracts were assessed by immunoblotting methods to measure the expression of a-tubulin, a microtubule protein critical for axonal transport. Our results will help us gain insight into a potential mechanism by which CPF affects axonal transport.
Affiliation:
Medical College of Georgia
Issue Date:
11-Aug-2015
URI:
http://hdl.handle.net/10675.2/565805
Type:
Presentation
Description:
This poster presentation was given at the 2015 CURS Summer Scholars Symposium
Sponsors:
Office of the Provost, VP for Academic and Faculty Affairs, Office of Research
Appears in Collections:
Summer Scholars Program

Full metadata record

DC FieldValue Language
dc.contributor.authorLalani, Ashishen
dc.contributor.authorMurphy, Shannonen
dc.contributor.authorBeck, W. Danen
dc.contributor.authorPoddar, Indranien
dc.contributor.authorTerry, Alvin V.en
dc.contributor.authorHernandez, Caterina M.en
dc.date.accessioned2015-08-11T02:31:43Zen
dc.date.available2015-08-11T02:31:43Zen
dc.date.issued2015-08-11en
dc.identifier.urihttp://hdl.handle.net/10675.2/565805en
dc.descriptionThis poster presentation was given at the 2015 CURS Summer Scholars Symposiumen
dc.description.abstractOrganophosphates are a class of chemicals that are used in pesticides, herbicides, and also as nerve agents. Organophosphate pesticides are ubiquitous among agricultural fields and the prolonged effects of organophosphates are not well understood. It is believed that exposure to Organophosphates can cause neurological deficits and impaired neurobehavioral function. We hypothesize that the organophosphate pesticide chlorpyrifos (CPF) is associated with mechanisms that directly and/or indirectly disrupt axonal transport in brain regions integral for learning and memory. To assay this claim, rats were treated acutely with CPF at a subtoxic dose (18 mg/kg) or with repeated exposures for 14 consecutive days at subtoxic doses (3.0, 10.0 or 18 mg/kg). Whole brain was collected 6 or 24 hours after acute exposure or directly following 14 days of repeated exposure or a 30-day drug-free washout period. For my project, I focused on the prefrontal cortex, a brain region important for executive function. Prefrontal cortex was processed using a subcellular fractionation protocol and extracts were assessed by immunoblotting methods to measure the expression of a-tubulin, a microtubule protein critical for axonal transport. Our results will help us gain insight into a potential mechanism by which CPF affects axonal transport.en
dc.description.sponsorshipOffice of the Provost, VP for Academic and Faculty Affairs, Office of Researchen
dc.subjectBrainen
dc.subjectOrganophosphateen
dc.titleThe Effects of Acute and Repeated Exposure/s to the Organophosphate Pesticide Chlorpyrifos at Subthreshold Doses on Brain Structural Integrityen
dc.typePresentationen
dc.contributor.departmentMedical College of Georgiaen
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