Rapid Reversal of Chondroitin Sulfate Proteoglycan Associated Staining in Subcompartments of Mouse Neostriatum during the Emergence of Behaviour

Hdl Handle:
http://hdl.handle.net/10675.2/565
Title:
Rapid Reversal of Chondroitin Sulfate Proteoglycan Associated Staining in Subcompartments of Mouse Neostriatum during the Emergence of Behaviour
Authors:
Lee, Hyunchul; Leamey, Catherine A.; Sawatari, Atomu
Abstract:
Background: The neostriatum, the mouse homologue of the primate caudate/putamen, is the input nucleus for the basal ganglia, receiving both cortical and dopaminergic input to each of its sub-compartments, the striosomes and matrix. The coordinated activation of corticostriatal pathways is considered vital for motor and cognitive abilities, yet the mechanisms which underlie the generation of these circuits are unknown. The early and specific targeting of striatal subcompartments by both corticostriatal and nigrostriatal terminals suggests activity-independent mechanisms, such as axon guidance cues, may play a role in this process. Candidates include the chondroitin sulfate proteoglycan (CSPG) family of glycoproteins which have roles not only in axon guidance, but also in the maturation and stability of neural circuits where they are expressed in lattice-like perineuronal nets (PNNs).; Methodology/Principal Findings: The expression of CSPG-associated structures and PNNs with respect to neostriatal subcompartments has been examined qualitatively and quantitatively using double-labelling for Wisteria floribunda agglutinin (WFA), and the μ-opioid receptor (μOR), a marker for striosomes, at six postnatal ages in mice. We find that at the earliest ages (postnatal day (P)4 and P10), WFA-positive clusters overlap preferentially with the striosome compartment. By P14, these clusters disappear. In contrast, PNNs were first seen at P10 and continued to increase in density and spread throughout the caudate/putamen with maturation. Remarkably, the PNNs overlap almost exclusively with the neostriatal matrix.; Conclusions/Significance: This is the first description of a reversal in the distribution of CSPG associated structures, as well as the emergence and maintenance of PNNs in specific subcompartments of the neostriatum. These results suggest diverse roles for CSPGs in the formation of functional corticostriatal and nigrostriatal connectivity within the striosome and matrix compartments of the developing caudate/putamen.
Citation:
PLoS ONE. 2008 Aug 20; 3(8):e3020
Issue Date:
20-Aug-2008
URI:
http://hdl.handle.net/10675.2/565
DOI:
10.1371/journal.pone.0003020
PubMed ID:
18714376
PubMed Central ID:
PMC2500190
Type:
Article
ISSN:
1932-6203
Appears in Collections:
Department of Neurology: Faculty Research and Presentations

Full metadata record

DC FieldValue Language
dc.contributor.authorLee, Hyunchulen_US
dc.contributor.authorLeamey, Catherine A.en_US
dc.contributor.authorSawatari, Atomuen_US
dc.date.accessioned2012-10-26T16:26:42Z-
dc.date.available2012-10-26T16:26:42Z-
dc.date.issued2008-08-20en_US
dc.identifier.citationPLoS ONE. 2008 Aug 20; 3(8):e3020en_US
dc.identifier.issn1932-6203en_US
dc.identifier.pmid18714376en_US
dc.identifier.doi10.1371/journal.pone.0003020en_US
dc.identifier.urihttp://hdl.handle.net/10675.2/565-
dc.description.abstractBackground: The neostriatum, the mouse homologue of the primate caudate/putamen, is the input nucleus for the basal ganglia, receiving both cortical and dopaminergic input to each of its sub-compartments, the striosomes and matrix. The coordinated activation of corticostriatal pathways is considered vital for motor and cognitive abilities, yet the mechanisms which underlie the generation of these circuits are unknown. The early and specific targeting of striatal subcompartments by both corticostriatal and nigrostriatal terminals suggests activity-independent mechanisms, such as axon guidance cues, may play a role in this process. Candidates include the chondroitin sulfate proteoglycan (CSPG) family of glycoproteins which have roles not only in axon guidance, but also in the maturation and stability of neural circuits where they are expressed in lattice-like perineuronal nets (PNNs).en_US
dc.description.abstractMethodology/Principal Findings: The expression of CSPG-associated structures and PNNs with respect to neostriatal subcompartments has been examined qualitatively and quantitatively using double-labelling for Wisteria floribunda agglutinin (WFA), and the μ-opioid receptor (μOR), a marker for striosomes, at six postnatal ages in mice. We find that at the earliest ages (postnatal day (P)4 and P10), WFA-positive clusters overlap preferentially with the striosome compartment. By P14, these clusters disappear. In contrast, PNNs were first seen at P10 and continued to increase in density and spread throughout the caudate/putamen with maturation. Remarkably, the PNNs overlap almost exclusively with the neostriatal matrix.en_US
dc.description.abstractConclusions/Significance: This is the first description of a reversal in the distribution of CSPG associated structures, as well as the emergence and maintenance of PNNs in specific subcompartments of the neostriatum. These results suggest diverse roles for CSPGs in the formation of functional corticostriatal and nigrostriatal connectivity within the striosome and matrix compartments of the developing caudate/putamen.en_US
dc.rightsLee et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.en_US
dc.subjectResearch Articleen_US
dc.subjectCell Biology/Developmental Molecular Mechanismsen_US
dc.subjectCell Biology/Neuronal Signaling Mechanismsen_US
dc.subjectDevelopmental Biology/Neurodevelopmenten_US
dc.subjectDevelopmental Biology/Pattern Formationen_US
dc.subjectNeuroscience/Motor Systemsen_US
dc.subjectNeuroscience/Neurodevelopmenten_US
dc.subjectNeuroscience/Neuronal and Glial Cell Biologyen_US
dc.subjectNeuroscience/Sensory Systemsen_US
dc.titleRapid Reversal of Chondroitin Sulfate Proteoglycan Associated Staining in Subcompartments of Mouse Neostriatum during the Emergence of Behaviouren_US
dc.typeArticleen_US
dc.identifier.pmcidPMC2500190en_US
dc.contributor.corporatenameDepartment of Neurology-
dc.contributor.corporatenameCollege of Graduate Studies-

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