Hdl Handle:
http://hdl.handle.net/10675.2/563
Title:
Ndel1 Promotes Axon Regeneration via Intermediate Filaments
Authors:
Toth, Cory; Shim, Su Yeon; Wang, Jian; Jiang, Yulan; Neumayer, Gernot; Belzil, Camille; Liu, Wei-Qiao; Martinez, Jose; Zochodne, Douglas; Nguyen, Minh Dang
Abstract:
Failure of axons to regenerate following acute or chronic neuronal injury is attributed to both the inhibitory glial environment and deficient intrinsic ability to re-grow. However, the underlying mechanisms of the latter remain unclear. In this study, we have investigated the role of the mammalian homologue of aspergillus nidulans NudE, Ndel1, emergently viewed as an integrator of the cytoskeleton, in axon regeneration. Ndel1 was synthesized de novo and upregulated in crushed and transected sciatic nerve axons, and, upon injury, was strongly associated with neuronal form of the intermediate filament (IF) Vimentin while dissociating from the mature neuronal IF (Neurofilament) light chain NF-L. Consistent with a role for Ndel1 in the conditioning lesion-induced neurite outgrowth of Dorsal Root Ganglion (DRG) neurons, the long lasting in vivo formation of the neuronal Ndel1/Vimentin complex was associated with robust axon regeneration. Furthermore, local silencing of Ndel1 in transected axons by siRNA severely reduced the extent of regeneration in vivo. Thus, Ndel1 promotes axonal regeneration; activating this endogenous repair mechanism may enhance neuroregeneration during acute and chronic axonal degeneration.
Editors:
Mei, Lin
Citation:
PLoS ONE. 2008 Apr 23; 3(4):e2014
Issue Date:
23-Apr-2008
URI:
http://hdl.handle.net/10675.2/563
DOI:
10.1371/journal.pone.0002014
PubMed ID:
18431495
PubMed Central ID:
PMC2291557
Type:
Article
ISSN:
1932-6203
Appears in Collections:
Department of Neurology: Faculty Research and Presentations

Full metadata record

DC FieldValue Language
dc.contributor.authorToth, Coryen_US
dc.contributor.authorShim, Su Yeonen_US
dc.contributor.authorWang, Jianen_US
dc.contributor.authorJiang, Yulanen_US
dc.contributor.authorNeumayer, Gernoten_US
dc.contributor.authorBelzil, Camilleen_US
dc.contributor.authorLiu, Wei-Qiaoen_US
dc.contributor.authorMartinez, Joseen_US
dc.contributor.authorZochodne, Douglasen_US
dc.contributor.authorNguyen, Minh Dangen_US
dc.contributor.editorMei, Lin-
dc.date.accessioned2012-10-26T16:26:42Z-
dc.date.available2012-10-26T16:26:42Z-
dc.date.issued2008-04-23en_US
dc.identifier.citationPLoS ONE. 2008 Apr 23; 3(4):e2014en_US
dc.identifier.issn1932-6203en_US
dc.identifier.pmid18431495en_US
dc.identifier.doi10.1371/journal.pone.0002014en_US
dc.identifier.urihttp://hdl.handle.net/10675.2/563-
dc.description.abstractFailure of axons to regenerate following acute or chronic neuronal injury is attributed to both the inhibitory glial environment and deficient intrinsic ability to re-grow. However, the underlying mechanisms of the latter remain unclear. In this study, we have investigated the role of the mammalian homologue of aspergillus nidulans NudE, Ndel1, emergently viewed as an integrator of the cytoskeleton, in axon regeneration. Ndel1 was synthesized de novo and upregulated in crushed and transected sciatic nerve axons, and, upon injury, was strongly associated with neuronal form of the intermediate filament (IF) Vimentin while dissociating from the mature neuronal IF (Neurofilament) light chain NF-L. Consistent with a role for Ndel1 in the conditioning lesion-induced neurite outgrowth of Dorsal Root Ganglion (DRG) neurons, the long lasting in vivo formation of the neuronal Ndel1/Vimentin complex was associated with robust axon regeneration. Furthermore, local silencing of Ndel1 in transected axons by siRNA severely reduced the extent of regeneration in vivo. Thus, Ndel1 promotes axonal regeneration; activating this endogenous repair mechanism may enhance neuroregeneration during acute and chronic axonal degeneration.en_US
dc.rightsToth et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.en_US
dc.subjectResearch Articleen_US
dc.subjectCell Biologyen_US
dc.subjectNeuroscienceen_US
dc.subjectCell Biology/Cell Signalingen_US
dc.subjectCell Biology/Cellular Death and Stress Responsesen_US
dc.subjectCell Biology/Cytoskeletonen_US
dc.subjectCell Biology/Morphogenesis and Cell Biologyen_US
dc.subjectCell Biology/Neuronal and Glial Cell Biologyen_US
dc.subjectNeuroscience/Neural Homeostasisen_US
dc.subjectNeuroscience/Neurobiology of Disease and Regenerationen_US
dc.subjectNeuroscience/Neurodevelopmenten_US
dc.subjectNeuroscience/Neuronal and Glial Cell Biologyen_US
dc.subjectNeuroscience/Neuronal Signaling Mechanismsen_US
dc.subjectNeurological Disorders/Alzheimer Diseaseen_US
dc.subjectNeurological Disorders/Movement Disordersen_US
dc.subjectNeurological Disorders/Multiple Sclerosis and Related Disordersen_US
dc.titleNdel1 Promotes Axon Regeneration via Intermediate Filamentsen_US
dc.typeArticleen_US
dc.identifier.pmcidPMC2291557en_US
dc.contributor.corporatenameDepartment of Neurology-
dc.contributor.corporatenameCollege of Graduate Studies-

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