Use of a microscope stage-mounted Nickel-63 microirradiator for real-time observation of the DNA double-strand break response.

Hdl Handle:
http://hdl.handle.net/10675.2/50
Title:
Use of a microscope stage-mounted Nickel-63 microirradiator for real-time observation of the DNA double-strand break response.
Authors:
Cao, Zhen; Kuhne, Wendy W.; Steeb, Jennifer; Merkley, Mark A.; Zhou, Yunfeng; Janata, Jiri; Dynan, William S. ( 0000-0002-4045-5662 )
Abstract:
Eukaryotic cells begin to assemble discrete, nucleoplasmic repair foci within seconds after the onset of exposure to ionizing radiation. Real-time imaging of this assembly has the potential to further our understanding of the effects of medical and environmental radiation exposure. Here, we describe a microirradiation system for targeted delivery of ionizing radiation to individual cells without the need for specialized facilities. The system consists of a 25-micron diameter electroplated Nickel-63 electrode, enveloped in a glass capillary and mounted in a micromanipulator. Because of the low energy of the beta radiation and the minute total amount of isotope present on the tip, the device can be safely handled with minimum precautions. We demonstrate the use of this system for tracking assembly of individual repair foci in real time in live U2OS human osteosarcoma cells. Results indicate that there is a subset of foci that appear and disappear rapidly, before a plateau level is reached approximately 30 min post-exposure. This subset of foci would not have been evident without real-time observation. The development of a microirradiation system that is compatible with a standard biomedical laboratory expands the potential for real-time investigation of the biological effects of ionizing radiation.
Citation:
Nucleic Acids Res. 2010 Aug 19; 38(14):e144
Issue Date:
12-Aug-2010
URI:
http://hdl.handle.net/10675.2/50
DOI:
10.1093/nar/gkq409
PubMed ID:
20484377
PubMed Central ID:
PMC2919731
Type:
Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.
ISSN:
1362-4962
Appears in Collections:
Institute of Molecular Medicine and Genetics: Faculty Research and Presentations

Full metadata record

DC FieldValue Language
dc.contributor.authorCao, Zhenen_US
dc.contributor.authorKuhne, Wendy W.en_US
dc.contributor.authorSteeb, Jenniferen_US
dc.contributor.authorMerkley, Mark A.en_US
dc.contributor.authorZhou, Yunfengen_US
dc.contributor.authorJanata, Jirien_US
dc.contributor.authorDynan, William S.en_US
dc.date.accessioned2010-09-24T21:26:47Z-
dc.date.available2010-09-24T21:26:47Z-
dc.date.issued2010-08-12en_US
dc.identifier.citationNucleic Acids Res. 2010 Aug 19; 38(14):e144en_US
dc.identifier.issn1362-4962en_US
dc.identifier.pmid20484377en_US
dc.identifier.doi10.1093/nar/gkq409en_US
dc.identifier.urihttp://hdl.handle.net/10675.2/50-
dc.description.abstractEukaryotic cells begin to assemble discrete, nucleoplasmic repair foci within seconds after the onset of exposure to ionizing radiation. Real-time imaging of this assembly has the potential to further our understanding of the effects of medical and environmental radiation exposure. Here, we describe a microirradiation system for targeted delivery of ionizing radiation to individual cells without the need for specialized facilities. The system consists of a 25-micron diameter electroplated Nickel-63 electrode, enveloped in a glass capillary and mounted in a micromanipulator. Because of the low energy of the beta radiation and the minute total amount of isotope present on the tip, the device can be safely handled with minimum precautions. We demonstrate the use of this system for tracking assembly of individual repair foci in real time in live U2OS human osteosarcoma cells. Results indicate that there is a subset of foci that appear and disappear rapidly, before a plateau level is reached approximately 30 min post-exposure. This subset of foci would not have been evident without real-time observation. The development of a microirradiation system that is compatible with a standard biomedical laboratory expands the potential for real-time investigation of the biological effects of ionizing radiation.en_US
dc.rightsThe PMC Open Access Subset is a relatively small part of the total collection of articles in PMC. Articles in the PMC Open Access Subset are still protected by copyright, but are made available under a Creative Commons or similar license that generally allows more liberal redistribution and reuse than a traditional copyrighted work. Please refer to the license statement in each article for specific terms of use. The license terms are not identical for all articles in this subset.en_US
dc.titleUse of a microscope stage-mounted Nickel-63 microirradiator for real-time observation of the DNA double-strand break response.en_US
dc.typeJournal Articleen_US
dc.typeResearch Support, N.I.H., Extramuralen_US
dc.typeResearch Support, Non-U.S. Gov'ten_US
dc.typeResearch Support, U.S. Gov't, Non-P.H.S.en_US
dc.identifier.pmcidPMC2919731en_US
dc.contributor.corporatenameInstitute of Molecular Medicine and Geneticsen_US
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