The Lack of AC1 Impairs the Inhibitory Effects of cAMP on Cell Mitigation and Proliferation in Pancreatic Cancer

Hdl Handle:
http://hdl.handle.net/10675.2/346406
Title:
The Lack of AC1 Impairs the Inhibitory Effects of cAMP on Cell Mitigation and Proliferation in Pancreatic Cancer
Authors:
Quinn, Sierra; Sabbatini, Maria
Abstract:
Introduction: Adenylyl cyclase (AC) is an important enzyme in signal transduction processes. This protein catalyzes the conversion of ATP to cAMP. We found five isoforms of AC in human pancreatic carcinoma HPAC cell line: AC1, AC3, AC6, AC7 and AC9. Both AC1 and AC3 are very important because they are up-regulated in HPAC cells. We also found that forskolin (FSK}, inhibits cell proliferation and cell migration but not cell invasion in HPAC cells. Objective: To silence AC1 and AC3 and determine their participation in the inhibitory effect of cAMP on cell migration and proliferation of HPAC cells. Results: To study the functional roles of AC1 and AC3, the expression of both isoforms were knocked-out using siRNA (human). The lack of either AC1 or AC3 proteins was assessed using Western-blotting. When testing for cell proliferation and cell migration, we found that the inhibitory effect of FSK was impaired in the presence of siRNA AC1, but not in the presence of siRNA AC3. Discussion: We conclude that AC1 mediates the inhibitory effect of cAMP in cell proliferation and migration. Begin Time: 26:09 End Time: 44:52
Affiliation:
College of Science and Mathematics
Issue Date:
6-Feb-2015
URI:
http://hdl.handle.net/10675.2/346406
Additional Links:
https://lecture.gru.edu/ess/echo/presentation/98494fb1-330a-447a-b69a-a7a7b5c95893
Type:
Presentation
Description:
This presentation was given at the CURS Brown Bag Seminar Series on February 6, 2015 on the GRU Summerville Campus.
Series/Report no.:
Spring; 2015
Sponsors:
Center for Undergraduate Research and Scholarship; College of Science and Mathematics; Department of Biological Sciences
Appears in Collections:
CURS Brown Bag Presentations

Full metadata record

DC FieldValue Language
dc.contributor.authorQuinn, Sierraen
dc.contributor.authorSabbatini, Mariaen
dc.date.accessioned2015-03-09T13:26:52Zen
dc.date.available2015-03-09T13:26:52Zen
dc.date.issued2015-02-06en
dc.identifier.urihttp://hdl.handle.net/10675.2/346406en
dc.descriptionThis presentation was given at the CURS Brown Bag Seminar Series on February 6, 2015 on the GRU Summerville Campus.en
dc.description.abstractIntroduction: Adenylyl cyclase (AC) is an important enzyme in signal transduction processes. This protein catalyzes the conversion of ATP to cAMP. We found five isoforms of AC in human pancreatic carcinoma HPAC cell line: AC1, AC3, AC6, AC7 and AC9. Both AC1 and AC3 are very important because they are up-regulated in HPAC cells. We also found that forskolin (FSK}, inhibits cell proliferation and cell migration but not cell invasion in HPAC cells. Objective: To silence AC1 and AC3 and determine their participation in the inhibitory effect of cAMP on cell migration and proliferation of HPAC cells. Results: To study the functional roles of AC1 and AC3, the expression of both isoforms were knocked-out using siRNA (human). The lack of either AC1 or AC3 proteins was assessed using Western-blotting. When testing for cell proliferation and cell migration, we found that the inhibitory effect of FSK was impaired in the presence of siRNA AC1, but not in the presence of siRNA AC3. Discussion: We conclude that AC1 mediates the inhibitory effect of cAMP in cell proliferation and migration. Begin Time: 26:09 End Time: 44:52en
dc.description.sponsorshipCenter for Undergraduate Research and Scholarship; College of Science and Mathematics; Department of Biological Sciencesen
dc.relation.ispartofseriesSpringen
dc.relation.ispartofseries2015en
dc.relation.urlhttps://lecture.gru.edu/ess/echo/presentation/98494fb1-330a-447a-b69a-a7a7b5c95893en
dc.subjectAdenylate cyclaseen
dc.subjectSignal Transductionen
dc.subjectPancreatic Neoplasmsen
dc.subjectCell Proliferationen
dc.subjectBlotting, Westernen
dc.subjectRNA, Small Interferingen
dc.titleThe Lack of AC1 Impairs the Inhibitory Effects of cAMP on Cell Mitigation and Proliferation in Pancreatic Canceren
dc.typePresentationen
dc.contributor.departmentCollege of Science and Mathematicsen
All Items in Scholarly Commons are protected by copyright, with all rights reserved, unless otherwise indicated.