Investigating the role of Hob1 in Repairing Double Stranded DNA Breaks in the Fission Yeast, Schizosaccharomyces pombe

Hdl Handle:
http://hdl.handle.net/10675.2/346333
Title:
Investigating the role of Hob1 in Repairing Double Stranded DNA Breaks in the Fission Yeast, Schizosaccharomyces pombe
Authors:
Ozturk, Sarah; Abdulovic-Cui, Amy
Abstract:
Mutations in DNA induce many diseases, including cancer. The human protein, Binl, has anticancer properties and interacts with proteins involved in maintaining DNA stability. Work completed at the GRU Cancer Center has shown that Binl is specifically involved in the nonhomologous end-joining pathway (NHEJ), a pathway that repairs DNA breaks. To complement this work, we are investigating the role of Hobl, the homolog of Binl in fission yeast, in NHEJ, If Hobl functions in a similar manner to Binl, then removal of Hobl from yeast should decrease the cells ability to repair breaks in the DNA. We are testing this hypothesis using a genetic yeast transformation protocol that measures how efficient the yeast are at converting a linear piece of DNA into a repaired circular piece of DNA. Our initial data showed that yeast lacking the HOB1gene are 10 fold effective at repairing the linear DNA compared to wildtype yeast. These data were surprising as they contradict our hypothesis and the data collected in human cells that lack Binl. We are currently repeating the experiment to verify our results. Together our research supports a negative role for Hobl in repairing DNA double strand breaks in the fission yeast. Begin Time: 06:50 End Time: 28:24
Affiliation:
College of Science and Mathematics
Issue Date:
20-Feb-2015
URI:
http://hdl.handle.net/10675.2/346333
Additional Links:
https://lecture.gru.edu/ess/echo/presentation/3975a0e7-d1f7-4d53-8cbb-a9a77ab01309
Type:
Presentation
Language:
en
Description:
Presentation given at the CURS Brown Bag Seminar series on February 20, 2015.
Series/Report no.:
Spring; 2015
Sponsors:
Center for Undergraduate Research and Scholarship, College of Science and Mathematics; Department of Biological Sciences, Department of Biochemistry & Molecular Biology
Appears in Collections:
CURS Brown Bag Presentations

Full metadata record

DC FieldValue Language
dc.contributor.authorOzturk, Sarahen
dc.contributor.authorAbdulovic-Cui, Amyen
dc.date.accessioned2015-03-09T13:00:03Zen
dc.date.available2015-03-09T13:00:03Zen
dc.date.issued2015-02-20en
dc.identifier.urihttp://hdl.handle.net/10675.2/346333en
dc.descriptionPresentation given at the CURS Brown Bag Seminar series on February 20, 2015.en
dc.description.abstractMutations in DNA induce many diseases, including cancer. The human protein, Binl, has anticancer properties and interacts with proteins involved in maintaining DNA stability. Work completed at the GRU Cancer Center has shown that Binl is specifically involved in the nonhomologous end-joining pathway (NHEJ), a pathway that repairs DNA breaks. To complement this work, we are investigating the role of Hobl, the homolog of Binl in fission yeast, in NHEJ, If Hobl functions in a similar manner to Binl, then removal of Hobl from yeast should decrease the cells ability to repair breaks in the DNA. We are testing this hypothesis using a genetic yeast transformation protocol that measures how efficient the yeast are at converting a linear piece of DNA into a repaired circular piece of DNA. Our initial data showed that yeast lacking the HOB1gene are 10 fold effective at repairing the linear DNA compared to wildtype yeast. These data were surprising as they contradict our hypothesis and the data collected in human cells that lack Binl. We are currently repeating the experiment to verify our results. Together our research supports a negative role for Hobl in repairing DNA double strand breaks in the fission yeast. Begin Time: 06:50 End Time: 28:24en
dc.description.sponsorshipCenter for Undergraduate Research and Scholarship, College of Science and Mathematics; Department of Biological Sciences, Department of Biochemistry & Molecular Biologyen
dc.language.isoenen
dc.relation.ispartofseriesSpringen
dc.relation.ispartofseries2015en
dc.relation.urlhttps://lecture.gru.edu/ess/echo/presentation/3975a0e7-d1f7-4d53-8cbb-a9a77ab01309en
dc.subjectDNAen
dc.subjectGRU Cancer Centeren
dc.subjectHoblen
dc.subjectBinlen
dc.subjectSaccharomyces cerevisiaeen
dc.subjectDNA Breaks, Double-Strandeden
dc.titleInvestigating the role of Hob1 in Repairing Double Stranded DNA Breaks in the Fission Yeast, Schizosaccharomyces pombeen
dc.typePresentationen
dc.contributor.departmentCollege of Science and Mathematicsen
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