Effect of the LAC Operator-Repressor Complex on HS2 Enhancer Function in K562 Erythroleukemia Cells

Hdl Handle:
http://hdl.handle.net/10675.2/343997
Title:
Effect of the LAC Operator-Repressor Complex on HS2 Enhancer Function in K562 Erythroleukemia Cells
Authors:
Ainol, Lincoyan S.
Abstract:
Given the characteristics of the lac operator-repressor complex, especially the fact that it is a strong transcriptional terminator, we have chosen to use this complex to determine if the transcripts initiated by the HS2 enhancer and elongated toward the cis linked promoter by a tracking and transcription mechanism might play a role in HS2 enhancer function. Our approach was to insert the lac operator sequence between the HS2 enhancer and the e-globin promoter that drives a CAT reporter gene. Our hypothesis is: If the HS2 enhancer acts through a looping mechanism, the reporter gene activity should not be affected upon the blockage of the intervening region with the lac operator-repressor complex. In contrast, if a tracking mechanism is involved, then HS2 enhancer function will be reduced by the transcriptional terminator placed in the intervening region. In our study the lac operator-repressor system has been chosen to test this hypothesis, since it has been shown to be useful as an on/off switch in the regulation of gene expression in mammalian cells in vitro. The results reported here indicate that in this system, the lac operator-repressor complex acted as an insulator that not only prevented the HS2 enhancer action inside the construct but also insulated the HS2-ep-CAT transcription unit in the tandem repeats, i.e., it prevented the HS2 enhancer from acting upon neighboring transcription units. Because the operator-repressor complex is a strong transcriptional terminator, our findings indicate that a tracking and transcription mechanism of the HS2 enhancer complex that contains RNA polymerases may be involved in HS2 enhancer function.
Affiliation:
Not Listed
Issue Date:
Oct-1998
URI:
http://hdl.handle.net/10675.2/343997
Additional Links:
http://ezproxy.gru.edu/login?url=http://search.proquest.com/docview/304464808?accountid=12365
Type:
Dissertation
Appears in Collections:
Theses and Dissertations

Full metadata record

DC FieldValue Language
dc.contributor.authorAinol, Lincoyan S.en
dc.date.accessioned2015-01-30T19:33:24Z-
dc.date.available2015-01-30T19:33:24Z-
dc.date.issued1998-10-
dc.identifier.urihttp://hdl.handle.net/10675.2/343997-
dc.description.abstractGiven the characteristics of the lac operator-repressor complex, especially the fact that it is a strong transcriptional terminator, we have chosen to use this complex to determine if the transcripts initiated by the HS2 enhancer and elongated toward the cis linked promoter by a tracking and transcription mechanism might play a role in HS2 enhancer function. Our approach was to insert the lac operator sequence between the HS2 enhancer and the e-globin promoter that drives a CAT reporter gene. Our hypothesis is: If the HS2 enhancer acts through a looping mechanism, the reporter gene activity should not be affected upon the blockage of the intervening region with the lac operator-repressor complex. In contrast, if a tracking mechanism is involved, then HS2 enhancer function will be reduced by the transcriptional terminator placed in the intervening region. In our study the lac operator-repressor system has been chosen to test this hypothesis, since it has been shown to be useful as an on/off switch in the regulation of gene expression in mammalian cells in vitro. The results reported here indicate that in this system, the lac operator-repressor complex acted as an insulator that not only prevented the HS2 enhancer action inside the construct but also insulated the HS2-ep-CAT transcription unit in the tandem repeats, i.e., it prevented the HS2 enhancer from acting upon neighboring transcription units. Because the operator-repressor complex is a strong transcriptional terminator, our findings indicate that a tracking and transcription mechanism of the HS2 enhancer complex that contains RNA polymerases may be involved in HS2 enhancer function.en
dc.relation.urlhttp://ezproxy.gru.edu/login?url=http://search.proquest.com/docview/304464808?accountid=12365en
dc.rightsCopyright protected. Unauthorized reproduction or use beyond the exceptions granted by the Fair Use clause of U.S. Copyright law may violate federal law.en
dc.subjectLac Repressorsen
dc.subjectHemoglobinen
dc.subjectK562 Cellsen
dc.titleEffect of the LAC Operator-Repressor Complex on HS2 Enhancer Function in K562 Erythroleukemia Cellsen
dc.typeDissertationen
dc.contributor.departmentNot Listeden
dc.description.advisorNot Listeden
dc.description.committeeNot Listeden
dc.description.degreeDoctor of Philosophy (Ph.D.)en
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