Schizophrenia Susceptibility Gene TMEM108 Regulates Excitatory Synapse Development and Transmission in the Dentate Gyrus

Hdl Handle:
http://hdl.handle.net/10675.2/324773
Title:
Schizophrenia Susceptibility Gene TMEM108 Regulates Excitatory Synapse Development and Transmission in the Dentate Gyrus
Authors:
Bates, Ryan
Abstract:
The primary purpose of this project is to understand the function of schizophrenia susceptibility gene Transmembrane Protein 108 (TMEM108) in the brain. First, we set out to understand the temporal and spatial expression of TMEME108 in the brain using RT-PCR, mice harboring the LacZ gene driven by the TMEM108 promoter and antibodies targeting the TMEM108 protein. Second, to address protein function mice containing a null mutation in the TMEM108 gene were generated. This mouse was used to determine the effect of TMEM108 deletion on the development of gross brain morphology. Third, we sought to understand the function of TMEM108 in synaptic development and physiology by examining the number and type of synapse as well as recording from brain slices lacking TMEM108. Fourth, we sought to understand the consequence of TMEM108 deletion on animal behavior.
Issue Date:
Jun-2014
URI:
http://hdl.handle.net/10675.2/324773
Additional Links:
http://ezproxy.gru.edu/login?url=http://search.proquest.com/docview/1562986132?accountid=12365
Type:
Dissertation
Language:
en
Appears in Collections:
Theses and Dissertations

Full metadata record

DC FieldValue Language
dc.contributor.authorBates, Ryanen
dc.date.accessioned2014-08-14T02:41:18Zen
dc.date.available2014-08-14T02:41:18Zen
dc.date.issued2014-06en
dc.identifier.urihttp://hdl.handle.net/10675.2/324773en
dc.description.abstractThe primary purpose of this project is to understand the function of schizophrenia susceptibility gene Transmembrane Protein 108 (TMEM108) in the brain. First, we set out to understand the temporal and spatial expression of TMEME108 in the brain using RT-PCR, mice harboring the LacZ gene driven by the TMEM108 promoter and antibodies targeting the TMEM108 protein. Second, to address protein function mice containing a null mutation in the TMEM108 gene were generated. This mouse was used to determine the effect of TMEM108 deletion on the development of gross brain morphology. Third, we sought to understand the function of TMEM108 in synaptic development and physiology by examining the number and type of synapse as well as recording from brain slices lacking TMEM108. Fourth, we sought to understand the consequence of TMEM108 deletion on animal behavior.en
dc.language.isoenen
dc.relation.urlhttp://ezproxy.gru.edu/login?url=http://search.proquest.com/docview/1562986132?accountid=12365en
dc.rightsCopyright protected. Unauthorized reproduction or use beyond the exceptions granted by the Fair Use clause of U.S. Copyright law may violate federal law.en
dc.subjectSchizophreniaen
dc.subjectMental Healthen
dc.subjectTMEM108en
dc.subjectBrain Developmenten
dc.titleSchizophrenia Susceptibility Gene TMEM108 Regulates Excitatory Synapse Development and Transmission in the Dentate Gyrusen_US
dc.typeDissertationen
dc.description.advisorMei, Linen
dc.description.committeeDhandapani, Krishana; McCluskey, Lynette; Xiong, Wen-Chen; Kozlowski, Daviden
dc.description.degreeDoctor of Philosophy (Ph.D.)en
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