Molecular Basis of Arenavirus Persistence: Genomic and Biological Characterization of Aggressive and Docile Strains of Lymphocytic Choriomeningitis Virus (LCMV)

Hdl Handle:
http://hdl.handle.net/10675.2/317043
Title:
Molecular Basis of Arenavirus Persistence: Genomic and Biological Characterization of Aggressive and Docile Strains of Lymphocytic Choriomeningitis Virus (LCMV)
Authors:
Chen, Minjie
Abstract:
Arenaviruses include several causative agents of hemorrhagic fever disease in humans. In addition, the prototypic arenavirus lymphocytic choriomeningitis virus (LCMV) is a superb model for the study of virus-host interactions, including the basis of viral persistence and associated diseases. The molecular mechanisms concerning the regulation and specific role of viral proteins in modulating arenavirus-host cell interactions associated either with an acute or persistent infection and associated disease remain little understood. In this project I have performed the genomic and biological characterization of LCMV strains Docile (persistent) and Aggressive (not persistent) recovered from cloned cDNA via reverse genetics. The results confirmed that the cloned viruses accurately recreated the in vivo phenotypes associated with the corresponding natural Docile and Aggressive viral isolates. In addition, we provide evidence that the ability of the Docile strain to persist is determined by the nature of both S and L RNA segments. Thus, our findings provide the foundation for studies aimed at gaining a detailed understanding of viral determinants of LCMV persistence in its natural host that may aid in the development of vaccines to prevent or treat the diseases caused by arenaviruses in humans.
Affiliation:
Department of Biochemistry and Molecular Biology
Issue Date:
Jun-2008
URI:
http://hdl.handle.net/10675.2/317043
Additional Links:
http://ezproxy.augusta.edu/login?url=http://search.proquest.com/docview/304403087?accountid=12365
Type:
Dissertation
Appears in Collections:
Theses and Dissertations; Department of Biochemistry and Molecular Biology Theses and Dissertations

Full metadata record

DC FieldValue Language
dc.contributor.authorChen, Minjieen
dc.date.accessioned2014-05-15T19:23:57Z-
dc.date.available2014-05-15T19:23:57Z-
dc.date.issued2008-06-
dc.identifier.urihttp://hdl.handle.net/10675.2/317043-
dc.description.abstractArenaviruses include several causative agents of hemorrhagic fever disease in humans. In addition, the prototypic arenavirus lymphocytic choriomeningitis virus (LCMV) is a superb model for the study of virus-host interactions, including the basis of viral persistence and associated diseases. The molecular mechanisms concerning the regulation and specific role of viral proteins in modulating arenavirus-host cell interactions associated either with an acute or persistent infection and associated disease remain little understood. In this project I have performed the genomic and biological characterization of LCMV strains Docile (persistent) and Aggressive (not persistent) recovered from cloned cDNA via reverse genetics. The results confirmed that the cloned viruses accurately recreated the in vivo phenotypes associated with the corresponding natural Docile and Aggressive viral isolates. In addition, we provide evidence that the ability of the Docile strain to persist is determined by the nature of both S and L RNA segments. Thus, our findings provide the foundation for studies aimed at gaining a detailed understanding of viral determinants of LCMV persistence in its natural host that may aid in the development of vaccines to prevent or treat the diseases caused by arenaviruses in humans.en
dc.relation.urlhttp://ezproxy.augusta.edu/login?url=http://search.proquest.com/docview/304403087?accountid=12365en
dc.rightsCopyright protected. Unauthorized reproduction or use beyond the exceptions granted by the Fair Use clause of U.S. Copyright law may violate federal law.en
dc.subjectViral infectionen
dc.subjectLCMVen
dc.subjectArenavirusesen
dc.subjectRNA virusen
dc.subjectT-cell responseen
dc.subjectReverse genetics techniqueen
dc.subjectViral persistenceen
dc.titleMolecular Basis of Arenavirus Persistence: Genomic and Biological Characterization of Aggressive and Docile Strains of Lymphocytic Choriomeningitis Virus (LCMV)-
dc.typeDissertationen
dc.contributor.departmentDepartment of Biochemistry and Molecular Biologyen
dc.description.advisorMoskofidis, Dimitriosen
dc.description.committeeMivechi, Nahid; Horuzsko, Anatolij; Ko, Lan; Floras-Rozas, Hernan; Philips, Andrew.en
dc.description.degreeDoctor of Philosophy (Ph.D.)en
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