Hdl Handle:
http://hdl.handle.net/10675.2/24
Title:
Molecular cloning and characterization of the mouse Acdp gene family.
Authors:
Wang, Cong-Yi; Yang, Ping; Shi, Jing-Da; Purohit, Sharad; Guo, Dehuang; An, Haiqian; Gu, Jian-Guo; Ling, Jennifer X; Dong, Zheng; She, Jin-Xiong
Abstract:
BACKGROUND: We have recently cloned and characterized a novel gene family named ancient conserved domain protein (ACDP) in humans. To facilitate the functional study of this novel gene family, we have cloned and characterized Acdp, the mouse homologue of the human ACDP gene family. RESULTS: The four Acdp genes (Acdp1, Acdp2, Acdp3 and Acdp4) contain 3,631 bp, 3,244 bp, 2,684 bp and 2,743 bp of cDNA sequences, and encode deduced proteins of 951, 874, 713 and 771 amino acids, respectively. The mouse Acdp genes showed very strong homologies (>90%) in both nucleotide and amino acid sequences to their human counterparts. In addition, both nucleotide and amino acid sequences within the Ancient Conserved Domain (ACD) are highly conserved in many different taxonomic species. Particularly, Acdp proteins showed very strong AA homologies to the bacteria CorC protein (35% AA identity with 55% homology), which is involved in magnesium and cobalt efflux. The Acdp genes are widely expressed in all tissues tested except for Acdp1, which is only highly expressed in the brain with low levels of expression in kidney and testis. Immunostaining of Acdp1 in hippocampus neurons revealed a predominant localization on the plasma membrane. CONCLUSION: The Acdp genes are evolutionarily conserved in diverse species and ubiquitously expressed throughout development and adult tissues suggesting that Acdp may be an essential gene. Acdp showed strong homology to bacteria CorC protein and predominantly localized on the plasma membrane. These results suggest that Acdp is probably a family of proteins involved in ion transport in mammalian cells
Citation:
BMC Genomics. 2004 Jan 15; 5:7
Issue Date:
11-May-2004
URI:
http://hdl.handle.net/10675.2/24
DOI:
10.1186/1471-2164-5-7
PubMed ID:
14723793
PubMed Central ID:
PMC340383
Type:
Journal Article; Research Support, Non-U.S. Gov't
ISSN:
1471-2164
Appears in Collections:
Center for Biotechnology and Genomic Medicine: Faculty Research and Presentations

Full metadata record

DC FieldValue Language
dc.contributor.authorWang, Cong-Yien_US
dc.contributor.authorYang, Pingen_US
dc.contributor.authorShi, Jing-Daen_US
dc.contributor.authorPurohit, Sharaden_US
dc.contributor.authorGuo, Dehuangen_US
dc.contributor.authorAn, Haiqianen_US
dc.contributor.authorGu, Jian-Guoen_US
dc.contributor.authorLing, Jennifer Xen_US
dc.contributor.authorDong, Zhengen_US
dc.contributor.authorShe, Jin-Xiongen_US
dc.date.accessioned2010-09-24T20:59:22Z-
dc.date.available2010-09-24T20:59:22Z-
dc.date.issued2004-05-11en_US
dc.identifier.citationBMC Genomics. 2004 Jan 15; 5:7en_US
dc.identifier.issn1471-2164en_US
dc.identifier.pmid14723793en_US
dc.identifier.doi10.1186/1471-2164-5-7en_US
dc.identifier.urihttp://hdl.handle.net/10675.2/24-
dc.description.abstractBACKGROUND: We have recently cloned and characterized a novel gene family named ancient conserved domain protein (ACDP) in humans. To facilitate the functional study of this novel gene family, we have cloned and characterized Acdp, the mouse homologue of the human ACDP gene family. RESULTS: The four Acdp genes (Acdp1, Acdp2, Acdp3 and Acdp4) contain 3,631 bp, 3,244 bp, 2,684 bp and 2,743 bp of cDNA sequences, and encode deduced proteins of 951, 874, 713 and 771 amino acids, respectively. The mouse Acdp genes showed very strong homologies (>90%) in both nucleotide and amino acid sequences to their human counterparts. In addition, both nucleotide and amino acid sequences within the Ancient Conserved Domain (ACD) are highly conserved in many different taxonomic species. Particularly, Acdp proteins showed very strong AA homologies to the bacteria CorC protein (35% AA identity with 55% homology), which is involved in magnesium and cobalt efflux. The Acdp genes are widely expressed in all tissues tested except for Acdp1, which is only highly expressed in the brain with low levels of expression in kidney and testis. Immunostaining of Acdp1 in hippocampus neurons revealed a predominant localization on the plasma membrane. CONCLUSION: The Acdp genes are evolutionarily conserved in diverse species and ubiquitously expressed throughout development and adult tissues suggesting that Acdp may be an essential gene. Acdp showed strong homology to bacteria CorC protein and predominantly localized on the plasma membrane. These results suggest that Acdp is probably a family of proteins involved in ion transport in mammalian cellsen_US
dc.rightsThe PMC Open Access Subset is a relatively small part of the total collection of articles in PMC. Articles in the PMC Open Access Subset are still protected by copyright, but are made available under a Creative Commons or similar license that generally allows more liberal redistribution and reuse than a traditional copyrighted work. Please refer to the license statement in each article for specific terms of use. The license terms are not identical for all articles in this subset.en_US
dc.subject.mesh3T3 Cellsen_US
dc.subject.meshAmino Acid Sequenceen_US
dc.subject.meshAnimalsen_US
dc.subject.meshBlotting, Northernen_US
dc.subject.meshBlotting, Westernen_US
dc.subject.meshBrain / metabolismen_US
dc.subject.meshCarrier Proteins / genetics / metabolismen_US
dc.subject.meshCell Lineen_US
dc.subject.meshCell Membrane / metabolismen_US
dc.subject.meshChromosome Mappingen_US
dc.subject.meshChromosomes, Mammalian / geneticsen_US
dc.subject.meshCloning, Molecularen_US
dc.subject.meshDNA, Complementary / chemistry / geneticsen_US
dc.subject.meshGene Expression Profilingen_US
dc.subject.meshHumansen_US
dc.subject.meshMaleen_US
dc.subject.meshMembrane Proteins / genetics / metabolismen_US
dc.subject.meshMiceen_US
dc.subject.meshMicroscopy, Confocalen_US
dc.subject.meshMolecular Sequence Dataen_US
dc.subject.meshMultigene Family / geneticsen_US
dc.subject.meshPC12 Cellsen_US
dc.subject.meshPhylogenyen_US
dc.subject.meshRatsen_US
dc.subject.meshSequence Alignmenten_US
dc.subject.meshSequence Analysis, DNAen_US
dc.subject.meshSequence Homology, Amino Aciden_US
dc.titleMolecular cloning and characterization of the mouse Acdp gene family.en_US
dc.typeJournal Articleen_US
dc.typeResearch Support, Non-U.S. Gov'ten_US
dc.identifier.pmcidPMC340383en_US
dc.contributor.corporatenameCenter for Biotechnology and Genomic Medicineen_US
dc.contributor.corporatenameDepartment of Cellular Biology and Anatomyen_US

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