Lack of correlation between the levels of soluble cytotoxic T-lymphocyte associated antigen-4 (CTLA-4) and the CT-60 genotypes.

Hdl Handle:
http://hdl.handle.net/10675.2/14
Title:
Lack of correlation between the levels of soluble cytotoxic T-lymphocyte associated antigen-4 (CTLA-4) and the CT-60 genotypes.
Authors:
Purohit, Sharad; Podolsky, Robert H.; Collins, Christin; Zheng, Weipeng; Schatz, Desmond; Muir, Andy; Hopkins, Diane; Huang, Yi-Hua; She, Jin-Xiong
Abstract:
BACKGROUND: Cytotoxic T lymphocyte-associated antigen-4 (CTLA-4) plays a critical role in downregulation of antigen-activated immune response and polymorphisms at the CTLA-4 gene have been shown to be associated with several autoimmune diseases including type-1 diabetes (T1D). The etiological mutation was mapped to the CT60-A/G single nucleotide polymorphism (SNP) that is believed to control the processing and production of soluble CTLA-4 (sCTLA-4). METHODS: We therefore determined sCTLA-4 protein levels in the sera from 82 T1D patients and 19 autoantibody positive (AbP) subjects and 117 autoantibody negative (AbN) controls using ELISA. The CT-60 SNP was genotyped for these samples by using PCR and restriction enzyme digestion of a 268 bp DNA segment containing the SNP. Genotyping of CT-60 SNP was confirmed by dye terminating sequencing reaction. RESULTS: Higher levels of sCTLA-4 were observed in T1D (2.24 ng/ml) and AbP (mean = 2.17 ng/ml) subjects compared to AbN controls (mean = 1.69 ng/ml) with the differences between these subjects becoming significant with age (p = 0.02). However, we found no correlation between sCTLA-4 levels and the CTLA-4 CT-60 SNP genotypes. CONCLUSION: Consistent with the higher serum sCTLA-4 levels observed in other autoimmune diseases, our results suggest that sCTLA-4 may be a risk factor for T1D. However, our results do not support the conclusion that the CT-60 SNP controls the expression of sCTLA-4.
Citation:
J Autoimmune Dis. 2005 Oct 31; 2:8
Issue Date:
24-Nov-2005
URI:
http://hdl.handle.net/10675.2/14
DOI:
10.1186/1740-2557-2-8
PubMed ID:
16259622
PubMed Central ID:
PMC1289290
Type:
Journal Article
ISSN:
1740-2557
Appears in Collections:
Center for Biotechnology and Genomic Medicine: Faculty Research and Presentations

Full metadata record

DC FieldValue Language
dc.contributor.authorPurohit, Sharaden_US
dc.contributor.authorPodolsky, Robert H.en_US
dc.contributor.authorCollins, Christinen_US
dc.contributor.authorZheng, Weipengen_US
dc.contributor.authorSchatz, Desmonden_US
dc.contributor.authorMuir, Andyen_US
dc.contributor.authorHopkins, Dianeen_US
dc.contributor.authorHuang, Yi-Huaen_US
dc.contributor.authorShe, Jin-Xiongen_US
dc.date.accessioned2010-09-24T20:59:19Z-
dc.date.available2010-09-24T20:59:19Z-
dc.date.issued2005-11-24en_US
dc.identifier.citationJ Autoimmune Dis. 2005 Oct 31; 2:8en_US
dc.identifier.issn1740-2557en_US
dc.identifier.pmid16259622en_US
dc.identifier.doi10.1186/1740-2557-2-8en_US
dc.identifier.urihttp://hdl.handle.net/10675.2/14-
dc.description.abstractBACKGROUND: Cytotoxic T lymphocyte-associated antigen-4 (CTLA-4) plays a critical role in downregulation of antigen-activated immune response and polymorphisms at the CTLA-4 gene have been shown to be associated with several autoimmune diseases including type-1 diabetes (T1D). The etiological mutation was mapped to the CT60-A/G single nucleotide polymorphism (SNP) that is believed to control the processing and production of soluble CTLA-4 (sCTLA-4). METHODS: We therefore determined sCTLA-4 protein levels in the sera from 82 T1D patients and 19 autoantibody positive (AbP) subjects and 117 autoantibody negative (AbN) controls using ELISA. The CT-60 SNP was genotyped for these samples by using PCR and restriction enzyme digestion of a 268 bp DNA segment containing the SNP. Genotyping of CT-60 SNP was confirmed by dye terminating sequencing reaction. RESULTS: Higher levels of sCTLA-4 were observed in T1D (2.24 ng/ml) and AbP (mean = 2.17 ng/ml) subjects compared to AbN controls (mean = 1.69 ng/ml) with the differences between these subjects becoming significant with age (p = 0.02). However, we found no correlation between sCTLA-4 levels and the CTLA-4 CT-60 SNP genotypes. CONCLUSION: Consistent with the higher serum sCTLA-4 levels observed in other autoimmune diseases, our results suggest that sCTLA-4 may be a risk factor for T1D. However, our results do not support the conclusion that the CT-60 SNP controls the expression of sCTLA-4.en_US
dc.rightsThe PMC Open Access Subset is a relatively small part of the total collection of articles in PMC. Articles in the PMC Open Access Subset are still protected by copyright, but are made available under a Creative Commons or similar license that generally allows more liberal redistribution and reuse than a traditional copyrighted work. Please refer to the license statement in each article for specific terms of use. The license terms are not identical for all articles in this subset.en_US
dc.titleLack of correlation between the levels of soluble cytotoxic T-lymphocyte associated antigen-4 (CTLA-4) and the CT-60 genotypes.en_US
dc.typeJournal Articleen_US
dc.identifier.pmcidPMC1289290en_US
dc.contributor.corporatenameCenter for Biotechnology and Genomic Medicineen_US

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