Usefulness of the paralens fluorescent microscope adaptor for the identification of mycobacteria in both field and laboratory settings.

Hdl Handle:
http://hdl.handle.net/10675.2/104
Title:
Usefulness of the paralens fluorescent microscope adaptor for the identification of mycobacteria in both field and laboratory settings.
Authors:
Kuhn, Walter; Armstrong, Derek; Atteberry, Suzanne; Dewbrey, Euline; Smith, Diane; Hooper, Nancy
Abstract:
The presence of acid-fast bacilli (AFB) in laboratories has traditionally been demonstrated using the fluorochrome method, which requires a fluorescent microscope or the Ziehl-Neelsen (ZN) method employing light microscopy. Low sensitivity of the ZN method and high costs of fluoroscopy make the need for a more effective means of diagnosis a top priority, especially in developing countries where the burden of tuberculosis is high. The QBC ParaLens attachment (QBC Diagnostic Inc., Port Matilda, PA) is a substitute for conventional fluoroscopy in the identification of AFB. To evaluate the efficacy of the ParaLens LED (light-emitting diode) system, the authors performed a two-part study, looking at usefulness, functionality and durability in urban/rural health clinics around the world, as well as in a controlled state public health laboratory setting. In the field, the ParaLens was durable and functioned well with various power sources and lighting conditions. Results from the state laboratory indicated agreement between standard fluorescent microscopy and fluorescent microscopy using the ParaLens. This adaptor is a welcome addition to laboratories in resource-limited settings as a useful alternative to conventional fluoroscopy for detection of mycobacterial species.
Citation:
Open Microbiol J. 2010 Apr 30; 4:30-33
Issue Date:
17-Jun-2010
URI:
http://hdl.handle.net/10675.2/104
DOI:
10.2174/1874285801004010030
PubMed ID:
20556200
PubMed Central ID:
PMC2885593
Type:
Journal Article
ISSN:
1874-2858
Appears in Collections:
Department of Emergency Medicine and Hospitalist Services: Faculty Research and Presentations

Full metadata record

DC FieldValue Language
dc.contributor.authorKuhn, Walteren_US
dc.contributor.authorArmstrong, Dereken_US
dc.contributor.authorAtteberry, Suzanneen_US
dc.contributor.authorDewbrey, Eulineen_US
dc.contributor.authorSmith, Dianeen_US
dc.contributor.authorHooper, Nancyen_US
dc.date.accessioned2010-09-24T22:03:20Z-
dc.date.available2010-09-24T22:03:20Z-
dc.date.issued2010-06-17en_US
dc.identifier.citationOpen Microbiol J. 2010 Apr 30; 4:30-33en_US
dc.identifier.issn1874-2858en_US
dc.identifier.pmid20556200en_US
dc.identifier.doi10.2174/1874285801004010030en_US
dc.identifier.urihttp://hdl.handle.net/10675.2/104-
dc.description.abstractThe presence of acid-fast bacilli (AFB) in laboratories has traditionally been demonstrated using the fluorochrome method, which requires a fluorescent microscope or the Ziehl-Neelsen (ZN) method employing light microscopy. Low sensitivity of the ZN method and high costs of fluoroscopy make the need for a more effective means of diagnosis a top priority, especially in developing countries where the burden of tuberculosis is high. The QBC ParaLens attachment (QBC Diagnostic Inc., Port Matilda, PA) is a substitute for conventional fluoroscopy in the identification of AFB. To evaluate the efficacy of the ParaLens LED (light-emitting diode) system, the authors performed a two-part study, looking at usefulness, functionality and durability in urban/rural health clinics around the world, as well as in a controlled state public health laboratory setting. In the field, the ParaLens was durable and functioned well with various power sources and lighting conditions. Results from the state laboratory indicated agreement between standard fluorescent microscopy and fluorescent microscopy using the ParaLens. This adaptor is a welcome addition to laboratories in resource-limited settings as a useful alternative to conventional fluoroscopy for detection of mycobacterial species.en_US
dc.rightsThe PMC Open Access Subset is a relatively small part of the total collection of articles in PMC. Articles in the PMC Open Access Subset are still protected by copyright, but are made available under a Creative Commons or similar license that generally allows more liberal redistribution and reuse than a traditional copyrighted work. Please refer to the license statement in each article for specific terms of use. The license terms are not identical for all articles in this subset.en_US
dc.titleUsefulness of the paralens fluorescent microscope adaptor for the identification of mycobacteria in both field and laboratory settings.en_US
dc.typeJournal Articleen_US
dc.identifier.pmcidPMC2885593en_US
dc.contributor.corporatenameDepartment of Emergency Medicineen_US

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