Heat shock response in CHO mammalian cells is controlled by a nonlinear stochastic process.

Hdl Handle:
http://hdl.handle.net/10675.2/96
Title:
Heat shock response in CHO mammalian cells is controlled by a nonlinear stochastic process.
Authors:
Lipan, Ovidiu; Navenot, Jean-Marc; Wang, Zixuan; Huang, Lei; Peiper, Stephen C
Abstract:
In many biological systems, the interactions that describe the coupling between different units in a genetic network are nonlinear and stochastic. We study the interplay between stochasticity and nonlinearity using the responses of Chinese hamster ovary (CHO) mammalian cells to different temperature shocks. The experimental data show that the mean value response of a cell population can be described by a mathematical expression (empirical law) which is valid for a large range of heat shock conditions. A nonlinear stochastic theoretical model was developed that explains the empirical law for the mean response. Moreover, the theoretical model predicts a specific biological probability distribution of responses for a cell population. The prediction was experimentally confirmed by measurements at the single-cell level. The computational approach can be used to study other nonlinear stochastic biological phenomena.
Citation:
PLoS Comput Biol. 2007 Oct 5; 3(10):e187
Issue Date:
30-Oct-2007
URI:
http://hdl.handle.net/10675.2/96
DOI:
10.1371/journal.pcbi.0030187
PubMed ID:
17922567
PubMed Central ID:
PMC2000973
Type:
Journal Article; Research Support, Non-U.S. Gov't
ISSN:
1553-7358
Appears in Collections:
Department of Pathology: Faculty Research and Presentations

Full metadata record

DC FieldValue Language
dc.contributor.authorLipan, Ovidiuen_US
dc.contributor.authorNavenot, Jean-Marcen_US
dc.contributor.authorWang, Zixuanen_US
dc.contributor.authorHuang, Leien_US
dc.contributor.authorPeiper, Stephen Cen_US
dc.date.accessioned2010-09-24T22:03:17Z-
dc.date.available2010-09-24T22:03:17Z-
dc.date.issued2007-10-30en_US
dc.identifier.citationPLoS Comput Biol. 2007 Oct 5; 3(10):e187en_US
dc.identifier.issn1553-7358en_US
dc.identifier.pmid17922567en_US
dc.identifier.doi10.1371/journal.pcbi.0030187en_US
dc.identifier.urihttp://hdl.handle.net/10675.2/96-
dc.description.abstractIn many biological systems, the interactions that describe the coupling between different units in a genetic network are nonlinear and stochastic. We study the interplay between stochasticity and nonlinearity using the responses of Chinese hamster ovary (CHO) mammalian cells to different temperature shocks. The experimental data show that the mean value response of a cell population can be described by a mathematical expression (empirical law) which is valid for a large range of heat shock conditions. A nonlinear stochastic theoretical model was developed that explains the empirical law for the mean response. Moreover, the theoretical model predicts a specific biological probability distribution of responses for a cell population. The prediction was experimentally confirmed by measurements at the single-cell level. The computational approach can be used to study other nonlinear stochastic biological phenomena.en_US
dc.rightsThe PMC Open Access Subset is a relatively small part of the total collection of articles in PMC. Articles in the PMC Open Access Subset are still protected by copyright, but are made available under a Creative Commons or similar license that generally allows more liberal redistribution and reuse than a traditional copyrighted work. Please refer to the license statement in each article for specific terms of use. The license terms are not identical for all articles in this subset.en_US
dc.subject.meshAnimalsen_US
dc.subject.meshCHO Cellsen_US
dc.subject.meshComputer Simulationen_US
dc.subject.meshCricetinaeen_US
dc.subject.meshCricetulusen_US
dc.subject.meshFemaleen_US
dc.subject.meshGene Expression Regulationen_US
dc.subject.meshHeat-Shock Proteins / analysisen_US
dc.subject.meshHeat-Shock Responseen_US
dc.subject.meshModels, Biologicalen_US
dc.subject.meshNonlinear Dynamicsen_US
dc.subject.meshNumerical Analysis, Computer-Assisteden_US
dc.subject.meshStochastic Processesen_US
dc.titleHeat shock response in CHO mammalian cells is controlled by a nonlinear stochastic process.en_US
dc.typeJournal Articleen_US
dc.typeResearch Support, Non-U.S. Gov'ten_US
dc.identifier.pmcidPMC2000973en_US
dc.contributor.corporatenameDepartment of Pathologyen_US
dc.contributor.corporatenameGHSU Cancer Centeren_US
dc.contributor.corporatenameImmunotherapy Centeren_US
dc.contributor.corporatenameDepartment of Radiologyen_US
dc.contributor.corporatenameCenter for Molecular Chaperone/Radiobiology & Cancer Virologyen_US

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