Single-Stranded Oligonucleotide Aptamer Binding to P-Selectin Inhibits Adhesion of Sickle Red Blood Cells and Leukocytes to Endothelial Cells in Sickle Cell Disease Model Mice: Novel Therapeutics for Vaso-occlusive Episodes

Hdl Handle:
http://hdl.handle.net/10675.2/931
Title:
Single-Stranded Oligonucleotide Aptamer Binding to P-Selectin Inhibits Adhesion of Sickle Red Blood Cells and Leukocytes to Endothelial Cells in Sickle Cell Disease Model Mice: Novel Therapeutics for Vaso-occlusive Episodes
Authors:
Gutsaeva, Diana R.; Parkerson, James B.; Schaub, Robert G.; Kurz, Jeffrey C.; Head, C. Alvin
Abstract:
Adhesive interactions between circulating red blood cells (RBC), leukocytes, and endothelial cells in post capillary venules have been implicated as a contributing factor to the pathogenesis of vaso-occlusion, the major cause of morbidity and mortality associated with sickle cell disease (SCD). Endothelial cell P-selectin, a member of the selectin family of cell adhesion molecules, plays a key role in leukocyte recruitment as well as in the adhesion of sickle RBC (sRBC) to the endothelium. The expression of P-selectin is elevated in SCD and the interaction of P-selectin and its ligands is likely to contribute to impairment of the microvascular flow and thereby to the development of painful vaso-occlusive episodes. Aptamers, short single-stranded oligonucleotides that fold into complex 3-D structures and bind to ligands, have been developed for a wide range of therapeutic targets. Although anti-P-selectin aptamers have been shown to inhibit leukocyte rolling in vitro and to display efficacy in mouse models for inflammation, anti-adhesion activity of anti-P-selectin aptamers has never been evaluated in SCD.
Publisher:
American Society of Hematology
Issue Date:
Dec-2009
URI:
http://hdl.handle.net/10675.2/931
Language:
en_US
Description:
Poster
Series/Report no.:
ASH;2009_H01
Sponsors:
Archemix Corp.
Appears in Collections:
Department of Anesthesiology and Perioperative Medicine: Faculty Research and Presentaions

Full metadata record

DC FieldValue Language
dc.contributor.authorGutsaeva, Diana R.-
dc.contributor.authorParkerson, James B.-
dc.contributor.authorSchaub, Robert G.-
dc.contributor.authorKurz, Jeffrey C.-
dc.contributor.authorHead, C. Alvin-
dc.date.accessioned2013-02-07T20:56:46Z-
dc.date.available2013-02-07T20:56:46Z-
dc.date.issued2009-12-
dc.identifier.urihttp://hdl.handle.net/10675.2/931-
dc.descriptionPosteren_US
dc.description.abstractAdhesive interactions between circulating red blood cells (RBC), leukocytes, and endothelial cells in post capillary venules have been implicated as a contributing factor to the pathogenesis of vaso-occlusion, the major cause of morbidity and mortality associated with sickle cell disease (SCD). Endothelial cell P-selectin, a member of the selectin family of cell adhesion molecules, plays a key role in leukocyte recruitment as well as in the adhesion of sickle RBC (sRBC) to the endothelium. The expression of P-selectin is elevated in SCD and the interaction of P-selectin and its ligands is likely to contribute to impairment of the microvascular flow and thereby to the development of painful vaso-occlusive episodes. Aptamers, short single-stranded oligonucleotides that fold into complex 3-D structures and bind to ligands, have been developed for a wide range of therapeutic targets. Although anti-P-selectin aptamers have been shown to inhibit leukocyte rolling in vitro and to display efficacy in mouse models for inflammation, anti-adhesion activity of anti-P-selectin aptamers has never been evaluated in SCD.en_US
dc.description.sponsorshipArchemix Corp.en_US
dc.language.isoen_USen_US
dc.publisherAmerican Society of Hematologyen_US
dc.relation.ispartofseriesASH;2009_H01-
dc.subjectSickle cell diseaseen_US
dc.subjectAptameren_US
dc.subjectP-selectinen_US
dc.subjectVaso-occlusionen_US
dc.titleSingle-Stranded Oligonucleotide Aptamer Binding to P-Selectin Inhibits Adhesion of Sickle Red Blood Cells and Leukocytes to Endothelial Cells in Sickle Cell Disease Model Mice: Novel Therapeutics for Vaso-occlusive Episodesen_US
dc.contributor.corporatenameMedical College of Georgiaen_US
dc.contributor.corporatenameDepartment of Anesthesiology and Perioperative Medicineen_US
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