Effects of chromium picolinate on glycemic control and kidney of the obese Zucker rat.

Hdl Handle:
http://hdl.handle.net/10675.2/85
Title:
Effects of chromium picolinate on glycemic control and kidney of the obese Zucker rat.
Authors:
Mozaffari, Mahmood S.; Abdelsayed, Rafik; Liu, Jun Yao; Wimborne, Hereward J. C.; El-Remessy, Azza B. ( 0000-0003-4386-1989 ) ; El-Marakby, Ahmed
Abstract:
ABSTRACT: BACKGROUND: Chromium picolinate (Cr(pic)3) is advocated as adjuvant therapy for impaired glycemic control, despite concerns for DNA damage. Potential toxicity of Cr(pic)3 should be greater for the kidney that accumulates chromium. Therefore, we tested the hypothesis that Cr(pic)3 treatment of obese Zucker rats (OZR) exacerbates renal abnormalities associated with dysglycemia. METHODS: Male OZR were treated with diets lacking or containing 5 and 10 mg/kg of chromium, as Cr(pic)3, for 20 weeks; lean Zucker rats (LZR) served as controls. Glycemic and renal effects of Cr(pic)3 were determined in the context of indices of oxidative stress and inflammation. RESULTS: The OZR displayed increased fasting plasma glucose and insulin in association with enlarged pancreatic islets exhibiting collagen and periodic acid Schiff-positive deposits compared to LZR; Cr(pic)3 treatment did not affect these parameters. The OZR, irrespective of Cr(pic)3, excreted more albumin than LZR. Also, other indices of renal function or histopathology were not affected by Cr(pic)3 treatment. Urinary excretion of 8-hydroxydeoxyguanosine (8-OHdG), an index of oxidative DNA damage, was greater in the OZR than LZR; dietary Cr(pic)3 treatment attenuated 8-OHdG excretion. However, immunostaining of kidney for 8-OHdG revealed similar staining pattern and intensity, despite significant renal accumulation of chromium in Cr(pic)3-treated groups. Finally, increased renal nitrotyrosine and cyclooxygenase-2 levels and urinary excretion of monocyte chemoattractant protein-1 of OZR were partially reversed by Cr(pic)3 treatment. CONCLUSION: Dietary Cr(pic)3 treatment of OZR does not beneficially influence glycemic status or increase the risk for oxidative DNA damage; rather, the treatment attenuates indices of oxidative stress and inflammation.
Citation:
Nutr Metab (Lond). 2009 Dec 10; 6:51
Issue Date:
30-Dec-2009
URI:
http://hdl.handle.net/10675.2/85
DOI:
10.1186/1743-7075-6-51
PubMed ID:
20003253
PubMed Central ID:
PMC2799425
Type:
Journal Article
ISSN:
1743-7075
Appears in Collections:
Department of Oral Biology Faculty Papers

Full metadata record

DC FieldValue Language
dc.contributor.authorMozaffari, Mahmood S.en_US
dc.contributor.authorAbdelsayed, Rafiken_US
dc.contributor.authorLiu, Jun Yaoen_US
dc.contributor.authorWimborne, Hereward J. C.en_US
dc.contributor.authorEl-Remessy, Azza B.en_US
dc.contributor.authorEl-Marakby, Ahmeden_US
dc.date.accessioned2010-09-24T21:55:17Z-
dc.date.available2010-09-24T21:55:17Z-
dc.date.issued2009-12-30en_US
dc.identifier.citationNutr Metab (Lond). 2009 Dec 10; 6:51en_US
dc.identifier.issn1743-7075en_US
dc.identifier.pmid20003253en_US
dc.identifier.doi10.1186/1743-7075-6-51en_US
dc.identifier.urihttp://hdl.handle.net/10675.2/85-
dc.description.abstractABSTRACT: BACKGROUND: Chromium picolinate (Cr(pic)3) is advocated as adjuvant therapy for impaired glycemic control, despite concerns for DNA damage. Potential toxicity of Cr(pic)3 should be greater for the kidney that accumulates chromium. Therefore, we tested the hypothesis that Cr(pic)3 treatment of obese Zucker rats (OZR) exacerbates renal abnormalities associated with dysglycemia. METHODS: Male OZR were treated with diets lacking or containing 5 and 10 mg/kg of chromium, as Cr(pic)3, for 20 weeks; lean Zucker rats (LZR) served as controls. Glycemic and renal effects of Cr(pic)3 were determined in the context of indices of oxidative stress and inflammation. RESULTS: The OZR displayed increased fasting plasma glucose and insulin in association with enlarged pancreatic islets exhibiting collagen and periodic acid Schiff-positive deposits compared to LZR; Cr(pic)3 treatment did not affect these parameters. The OZR, irrespective of Cr(pic)3, excreted more albumin than LZR. Also, other indices of renal function or histopathology were not affected by Cr(pic)3 treatment. Urinary excretion of 8-hydroxydeoxyguanosine (8-OHdG), an index of oxidative DNA damage, was greater in the OZR than LZR; dietary Cr(pic)3 treatment attenuated 8-OHdG excretion. However, immunostaining of kidney for 8-OHdG revealed similar staining pattern and intensity, despite significant renal accumulation of chromium in Cr(pic)3-treated groups. Finally, increased renal nitrotyrosine and cyclooxygenase-2 levels and urinary excretion of monocyte chemoattractant protein-1 of OZR were partially reversed by Cr(pic)3 treatment. CONCLUSION: Dietary Cr(pic)3 treatment of OZR does not beneficially influence glycemic status or increase the risk for oxidative DNA damage; rather, the treatment attenuates indices of oxidative stress and inflammation.en_US
dc.rightsThe PMC Open Access Subset is a relatively small part of the total collection of articles in PMC. Articles in the PMC Open Access Subset are still protected by copyright, but are made available under a Creative Commons or similar license that generally allows more liberal redistribution and reuse than a traditional copyrighted work. Please refer to the license statement in each article for specific terms of use. The license terms are not identical for all articles in this subset.en_US
dc.titleEffects of chromium picolinate on glycemic control and kidney of the obese Zucker rat.en_US
dc.typeJournal Articleen_US
dc.identifier.pmcidPMC2799425en_US
dc.contributor.corporatenameDepartment of Oral Biologyen_US
dc.contributor.corporatenameDepartment of Oral Health and Diagnostic Sciencesen_US

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