Ontological Differences in First Compared to Third Trimester Human Fetal Placental Chorionic Stem Cells

Hdl Handle:
http://hdl.handle.net/10675.2/824
Title:
Ontological Differences in First Compared to Third Trimester Human Fetal Placental Chorionic Stem Cells
Authors:
Jones, Gemma N.; Moschidou, Dafni; Puga-Iglesias, Tamara-Isabel; Kuleszewicz, Katarzyna; Vanleene, Maximilien; Shefelbine, Sandra J.; Bou-Gharios, George; Fisk, Nicholas M. ( 0000-0003-0031-7975 ) ; David, Anna L. ( 0000-0002-0199-6140 ) ; De Coppi, Paolo ( 0000-0002-1659-0207 ) ; Guillot, Pascale V.
Abstract:
Human mesenchymal stromal/stem cells (MSC) isolated from fetal tissues hold promise for use in tissue engineering applications and cell-based therapies, but their collection is restricted ethically and technically. In contrast, the placenta is a potential source of readily-obtainable stem cells throughout pregnancy. In fetal tissues, early gestational stem cells are known to have advantageous characteristics over neonatal and adult stem cells. Accordingly, we investigated whether early fetal placental chorionic stem cells (e-CSC) were physiologically superior to their late gestation fetal chorionic counterparts (l-CSC). We showed that e-CSC shared a common phenotype with l-CSC, differentiating down the osteogenic, adipogenic and neurogenic pathways, and containing a subset of cells endogenously expressing NANOG, SOX2, c-MYC, and KLF4, as well as an array of genes expressed in pluripotent stem cells and primordial germ cells, including CD24, NANOG, SSEA4, SSEA3, TRA-1-60, TRA-1-81, STELLA, FRAGILIS, NANOS3, DAZL and SSEA1. However, we showed that e-CSC have characteristics of an earlier state of stemness compared to l-CSC, such as smaller size, faster kinetics, uniquely expressing OCT4A variant 1 and showing higher levels of expression of NANOG, SOX2, c-MYC and KLF4 than l-CSC. Furthermore e-CSC, but not l-CSC, formed embryoid bodies containing cells from the three germ layer lineages. Finally, we showed that e-CSC demonstrate higher tissue repair in vivo; when transplanted in the osteogenesis imperfecta mice, e-CSC, but not l-CSC increased bone quality and plasticity; and when applied to a skin wound, e-CSC, but not l-CSC, accelerated healing compared to controls. Our results provide insight into the ontogeny of the stemness phenotype during fetal development and suggest that the more primitive characteristics of early compared to late gestation fetal chorionic stem cells may be translationally advantageous.
Editors:
Shi, Xing-Ming
Citation:
PLoS One. 2012 Sep 4; 7(9):e43395
Issue Date:
4-Sep-2012
URI:
http://hdl.handle.net/10675.2/824
DOI:
10.1371/journal.pone.0043395
PubMed ID:
22962584
PubMed Central ID:
PMC3433473
Type:
Article
ISSN:
1932-6203
Appears in Collections:
Department of Pathology: Faculty Research and Presentations

Full metadata record

DC FieldValue Language
dc.contributor.authorJones, Gemma N.en_US
dc.contributor.authorMoschidou, Dafnien_US
dc.contributor.authorPuga-Iglesias, Tamara-Isabelen_US
dc.contributor.authorKuleszewicz, Katarzynaen_US
dc.contributor.authorVanleene, Maximilienen_US
dc.contributor.authorShefelbine, Sandra J.en_US
dc.contributor.authorBou-Gharios, Georgeen_US
dc.contributor.authorFisk, Nicholas M.en_US
dc.contributor.authorDavid, Anna L.en_US
dc.contributor.authorDe Coppi, Paoloen_US
dc.contributor.authorGuillot, Pascale V.en_US
dc.contributor.editorShi, Xing-Ming-
dc.date.accessioned2012-10-26T20:35:11Z-
dc.date.available2012-10-26T20:35:11Z-
dc.date.issued2012-09-4en_US
dc.identifier.citationPLoS One. 2012 Sep 4; 7(9):e43395en_US
dc.identifier.issn1932-6203en_US
dc.identifier.pmid22962584en_US
dc.identifier.doi10.1371/journal.pone.0043395en_US
dc.identifier.urihttp://hdl.handle.net/10675.2/824-
dc.description.abstractHuman mesenchymal stromal/stem cells (MSC) isolated from fetal tissues hold promise for use in tissue engineering applications and cell-based therapies, but their collection is restricted ethically and technically. In contrast, the placenta is a potential source of readily-obtainable stem cells throughout pregnancy. In fetal tissues, early gestational stem cells are known to have advantageous characteristics over neonatal and adult stem cells. Accordingly, we investigated whether early fetal placental chorionic stem cells (e-CSC) were physiologically superior to their late gestation fetal chorionic counterparts (l-CSC). We showed that e-CSC shared a common phenotype with l-CSC, differentiating down the osteogenic, adipogenic and neurogenic pathways, and containing a subset of cells endogenously expressing NANOG, SOX2, c-MYC, and KLF4, as well as an array of genes expressed in pluripotent stem cells and primordial germ cells, including CD24, NANOG, SSEA4, SSEA3, TRA-1-60, TRA-1-81, STELLA, FRAGILIS, NANOS3, DAZL and SSEA1. However, we showed that e-CSC have characteristics of an earlier state of stemness compared to l-CSC, such as smaller size, faster kinetics, uniquely expressing OCT4A variant 1 and showing higher levels of expression of NANOG, SOX2, c-MYC and KLF4 than l-CSC. Furthermore e-CSC, but not l-CSC, formed embryoid bodies containing cells from the three germ layer lineages. Finally, we showed that e-CSC demonstrate higher tissue repair in vivo; when transplanted in the osteogenesis imperfecta mice, e-CSC, but not l-CSC increased bone quality and plasticity; and when applied to a skin wound, e-CSC, but not l-CSC, accelerated healing compared to controls. Our results provide insight into the ontogeny of the stemness phenotype during fetal development and suggest that the more primitive characteristics of early compared to late gestation fetal chorionic stem cells may be translationally advantageous.en_US
dc.subjectResearch Articleen_US
dc.subjectBiologyen_US
dc.subjectDevelopmental Biologyen_US
dc.subjectStem Cellsen_US
dc.subjectCell Potencyen_US
dc.subjectMesenchymal Stem Cellsen_US
dc.subjectCell Differentiationen_US
dc.subjectEvolutionary Developmental Biologyen_US
dc.subjectMolecular Cell Biologyen_US
dc.subjectCellular Typesen_US
dc.subjectStem Cellsen_US
dc.subjectCell Potencyen_US
dc.subjectMesenchymal Stem Cellsen_US
dc.subjectCell Adhesionen_US
dc.subjectCell Growthen_US
dc.titleOntological Differences in First Compared to Third Trimester Human Fetal Placental Chorionic Stem Cellsen_US
dc.typeArticleen_US
dc.identifier.pmcidPMC3433473en_US
dc.contributor.corporatenameDepartment of Pathology-
dc.contributor.corporatenameCollege of Graduate Studies-

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