Hdl Handle:
http://hdl.handle.net/10675.2/773
Title:
hElp3 Directly Modulates the Expression of HSP70 Gene in HeLa Cells via HAT Activity
Authors:
Li, Fen; Ma, Jixian; Ma, Yu; Hu, Yanyan; Tian, Shujuan; White, Richard E.; Han, Guichun
Abstract:
Human Elongator complex, which plays a key role in transcript elongation in vitro assay, is incredibly similar in either components or function to its yeast counterpart. However, there are only a few studies focusing on its target gene characterization in vivo. We studied the effect of down-regulation of the human elongation protein 3 (hELP3) on the expression of HSP70 through antisense strategy. Transfecting antisense plasmid p1107 into HeLa cells highly suppressed hELP3 expression, and substantially reduced expression of HSP70 mRNA and protein. Furthermore, chromatin immunoprecipitation assay (ChIP Assay) revealed that hElp3 participates in the transcription elongation of HSPA1A in HeLa cells. Finally, complementation and ChIP Assay in yeast showed that hElp3 can not only complement the growth and slow activation of HSP70 (SSA3) gene transcription, but also directly regulates the transcription of SSA3. On the contrary, these functions are lost when the HAT domain is deleted from hElp3. These data suggest that hElp3 can regulate the transcription of HSP70 gene, and the HAT domain of hElp3 is essential for this function. These findings now provide novel insights and evidence of the functions of hELP3 in human cells.
Citation:
PLoS One. 2011 Dec 21; 6(12):e29303
Issue Date:
21-Dec-2011
URI:
http://hdl.handle.net/10675.2/773
DOI:
10.1371/journal.pone.0029303
PubMed ID:
22216241
PubMed Central ID:
PMC3244451
Type:
Article
ISSN:
1932-6203
Appears in Collections:
Department of Pharmacology and Toxicology: Faculty Research and Presentations

Full metadata record

DC FieldValue Language
dc.contributor.authorLi, Fenen_US
dc.contributor.authorMa, Jixianen_US
dc.contributor.authorMa, Yuen_US
dc.contributor.authorHu, Yanyanen_US
dc.contributor.authorTian, Shujuanen_US
dc.contributor.authorWhite, Richard E.en_US
dc.contributor.authorHan, Guichunen_US
dc.date.accessioned2012-10-26T20:30:42Z-
dc.date.available2012-10-26T20:30:42Z-
dc.date.issued2011-12-21en_US
dc.identifier.citationPLoS One. 2011 Dec 21; 6(12):e29303en_US
dc.identifier.issn1932-6203en_US
dc.identifier.pmid22216241en_US
dc.identifier.doi10.1371/journal.pone.0029303en_US
dc.identifier.urihttp://hdl.handle.net/10675.2/773-
dc.description.abstractHuman Elongator complex, which plays a key role in transcript elongation in vitro assay, is incredibly similar in either components or function to its yeast counterpart. However, there are only a few studies focusing on its target gene characterization in vivo. We studied the effect of down-regulation of the human elongation protein 3 (hELP3) on the expression of HSP70 through antisense strategy. Transfecting antisense plasmid p1107 into HeLa cells highly suppressed hELP3 expression, and substantially reduced expression of HSP70 mRNA and protein. Furthermore, chromatin immunoprecipitation assay (ChIP Assay) revealed that hElp3 participates in the transcription elongation of HSPA1A in HeLa cells. Finally, complementation and ChIP Assay in yeast showed that hElp3 can not only complement the growth and slow activation of HSP70 (SSA3) gene transcription, but also directly regulates the transcription of SSA3. On the contrary, these functions are lost when the HAT domain is deleted from hElp3. These data suggest that hElp3 can regulate the transcription of HSP70 gene, and the HAT domain of hElp3 is essential for this function. These findings now provide novel insights and evidence of the functions of hELP3 in human cells.en_US
dc.rightsLi et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.en_US
dc.subjectResearch Articleen_US
dc.subjectBiologyen_US
dc.subjectBiochemistryen_US
dc.subjectNucleic Acidsen_US
dc.subjectRNAen_US
dc.subjectRNA synthesisen_US
dc.subjectBiophysicsen_US
dc.subjectNucleic Acidsen_US
dc.subjectRNAen_US
dc.subjectRNA synthesisen_US
dc.subjectComputational Biologyen_US
dc.subjectMolecular Geneticsen_US
dc.subjectGene Expressionen_US
dc.subjectGeneticsen_US
dc.subjectGene Expressionen_US
dc.subjectDNA transcriptionen_US
dc.subjectMolecular Cell Biologyen_US
dc.subjectGene Expressionen_US
dc.subjectDNA transcriptionen_US
dc.subjectNucleic Acidsen_US
dc.subjectRNAen_US
dc.subjectRNA synthesisen_US
dc.subjectPhysicsen_US
dc.subjectBiophysicsen_US
dc.subjectNucleic Acidsen_US
dc.subjectRNAen_US
dc.subjectRNA synthesisen_US
dc.titlehElp3 Directly Modulates the Expression of HSP70 Gene in HeLa Cells via HAT Activityen_US
dc.typeArticleen_US
dc.identifier.pmcidPMC3244451en_US
dc.contributor.corporatenameDepartment of Pharmacology and Toxicology-

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