High-mobility group box-1 and biomarkers of inflammation in the vitreous from patients with proliferative diabetic retinopathy

Hdl Handle:
http://hdl.handle.net/10675.2/749
Title:
High-mobility group box-1 and biomarkers of inflammation in the vitreous from patients with proliferative diabetic retinopathy
Authors:
El-Asrar, Ahmed M. Abu; Nawaz, Mohd Imtiaz; Kangave, Dustan; Geboes, Karel; Ola, Mohammad Shamsul; Ahmad, Saif; Al-Shabrawey, Mohamed ( 0000-0001-5362-7972 )
Abstract:
Purpose: To measure levels of high-mobility group box −1 (HMGB1) and soluble receptor for advanced glycation end products (sRAGE) in the vitreous fluid from patients with proliferative diabetic retinopathy (PDR) and to correlate their levels with clinical disease activity and the levels of the inflammatory biomarkers monocyte chemoattractant protein-1 (MCP-1), soluble intercellular adhesion molecule-1 (sICAM-1), interleukin-1β (IL-1β), and granulocyte macrophage colony-stimulating factor (GM-CSF). In addition, we examined the expression of HMGB1 in the retinas of diabetic mice.; Methods: Vitreous samples from 29 PDR and 17 nondiabetic patients were studied by enzyme-linked immunosorbent assay. Retinas of mice were examined by immunofluorescence analysis and western blotting.; Results: HMGB1 was detected in all vitreous samples and sRAGE was detected in 5 PDR samples. IL-1β was detected in 3PDR samples and GM-CSF was not detected. Mean HMGB1 levels in PDR with active neovascularization were twofold and threefold higher than that in inactive PDR and nondiabetic patients, respectively. Mean HMGB1 levels in PDR patients with hemorrhage were significantly higher than those in PDR patients without hemorrhage and nondiabetic patients (p=0.0111). There were significant correlations between levels of HMGB1 and levels of MCP-1 (r=0.333, p=0.025) and sICAM-1 (r=0.548, p<0.001). HMGB1 expression was also upregulated in the retinas of diabetic mice.; Conclusions: Subclinical chronic inflammation might contribute to the progression of PDR.
Citation:
Mol Vis. 2011 Jul 6; 17:1829-1838
Issue Date:
6-Jul-2011
URI:
http://hdl.handle.net/10675.2/749
PubMed ID:
21850157
PubMed Central ID:
PMC3137555
Type:
Article
ISSN:
1090-0535
Appears in Collections:
Department of Oral Biology: Faculty Research and Publications

Full metadata record

DC FieldValue Language
dc.contributor.authorEl-Asrar, Ahmed M. Abuen_US
dc.contributor.authorNawaz, Mohd Imtiazen_US
dc.contributor.authorKangave, Dustanen_US
dc.contributor.authorGeboes, Karelen_US
dc.contributor.authorOla, Mohammad Shamsulen_US
dc.contributor.authorAhmad, Saifen_US
dc.contributor.authorAl-Shabrawey, Mohameden_US
dc.date.accessioned2012-10-26T20:27:56Z-
dc.date.available2012-10-26T20:27:56Z-
dc.date.issued2011-07-06en_US
dc.identifier.citationMol Vis. 2011 Jul 6; 17:1829-1838en_US
dc.identifier.issn1090-0535en_US
dc.identifier.pmid21850157en_US
dc.identifier.urihttp://hdl.handle.net/10675.2/749-
dc.description.abstractPurpose: To measure levels of high-mobility group box −1 (HMGB1) and soluble receptor for advanced glycation end products (sRAGE) in the vitreous fluid from patients with proliferative diabetic retinopathy (PDR) and to correlate their levels with clinical disease activity and the levels of the inflammatory biomarkers monocyte chemoattractant protein-1 (MCP-1), soluble intercellular adhesion molecule-1 (sICAM-1), interleukin-1β (IL-1β), and granulocyte macrophage colony-stimulating factor (GM-CSF). In addition, we examined the expression of HMGB1 in the retinas of diabetic mice.en_US
dc.description.abstractMethods: Vitreous samples from 29 PDR and 17 nondiabetic patients were studied by enzyme-linked immunosorbent assay. Retinas of mice were examined by immunofluorescence analysis and western blotting.en_US
dc.description.abstractResults: HMGB1 was detected in all vitreous samples and sRAGE was detected in 5 PDR samples. IL-1β was detected in 3PDR samples and GM-CSF was not detected. Mean HMGB1 levels in PDR with active neovascularization were twofold and threefold higher than that in inactive PDR and nondiabetic patients, respectively. Mean HMGB1 levels in PDR patients with hemorrhage were significantly higher than those in PDR patients without hemorrhage and nondiabetic patients (p=0.0111). There were significant correlations between levels of HMGB1 and levels of MCP-1 (r=0.333, p=0.025) and sICAM-1 (r=0.548, p<0.001). HMGB1 expression was also upregulated in the retinas of diabetic mice.en_US
dc.description.abstractConclusions: Subclinical chronic inflammation might contribute to the progression of PDR.en_US
dc.rightsCopyright © 2011 Molecular Vision.en_US
dc.subjectResearch Articleen_US
dc.titleHigh-mobility group box-1 and biomarkers of inflammation in the vitreous from patients with proliferative diabetic retinopathyen_US
dc.typeArticleen_US
dc.identifier.pmcidPMC3137555en_US
dc.contributor.corporatenameDepartment of Oral Biology-
dc.contributor.corporatenameDepartment of Ophthalmology-

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