Caspase-14: A novel caspase in the retina with a potential role in diabetic retinopathy

Hdl Handle:
http://hdl.handle.net/10675.2/724
Title:
Caspase-14: A novel caspase in the retina with a potential role in diabetic retinopathy
Authors:
Al-Shabrawey, Mohamed ( 0000-0001-5362-7972 ) ; Ahmad, Saif; Megyerdi, Sylvia; Othman, Amira; Baban, Babak; Palenski, Tammy L.; Shin, Eui Seok; Gurel, Zafer; Hsu, Stephen; Sheibani, Nader
Abstract:
Purpose: The purpose of this study was to evaluate caspase-14 expression in the retina under normal and diabetic conditions, and to determine whether caspase-14 contributes to retinal microvascular cell death under high glucose conditions.; Methods: Quantitative real-time polymerase chain reaction and western blot analysis were used to evaluate caspase-14 expression in retinal cells, including pericytes (PCs), endothelial cells (ECs), astrocytes (ACs), choroidal ECs, and retinal pigment epithelium (RPE) cells. We also determined caspase-14 expression in the retinas of human subjects with or without diabetic retinopathy (DR) and in experimental diabetic mice. Retinal ECs and PCs were infected with adenoviruses expressing human caspase-14 or green fluorescent protein. Caspase-14 expression was also assessed in retinal vascular cells cultured under high glucose conditions. The number of apoptotic cells was determined with terminal deoxynucleotidyl transferase dUTP nick end labeling staining and confirmed by determining the levels of cleaved poly (ADP-ribose) polymerase-1 and caspase-3.; Results: Our experiments demonstrated that retinal ECs, PCs, ACs, choroidal ECs, and RPE cells expressed caspase-14, and DR was associated with upregulation and/or activation of caspase-14 particularly in retinal vasculature. High glucose induced marked elevation of the caspase-14 level in retinal vascular cells. There was a significant increase in the apoptosis rate and the levels of cleaved poly (ADP-ribose) polymerase-1 and caspase-3 in retinal ECs and PCs overexpressing caspase-14.; Conclusions: Our findings indicate that caspase-14 might play a significant role in the pathogenesis of DR by accelerating retinal PC and EC death. Further investigations are required to elaborate the underlying mechanisms.
Citation:
Mol Vis. 2012 Jul 14; 18:1895-1906
Issue Date:
14-Jul-2012
URI:
http://hdl.handle.net/10675.2/724
PubMed ID:
22876114
PubMed Central ID:
PMC3413417
Type:
Article
ISSN:
1090-0535
Appears in Collections:
Department of Oral Biology: Faculty Research and Publications

Full metadata record

DC FieldValue Language
dc.contributor.authorAl-Shabrawey, Mohameden_US
dc.contributor.authorAhmad, Saifen_US
dc.contributor.authorMegyerdi, Sylviaen_US
dc.contributor.authorOthman, Amiraen_US
dc.contributor.authorBaban, Babaken_US
dc.contributor.authorPalenski, Tammy L.en_US
dc.contributor.authorShin, Eui Seoken_US
dc.contributor.authorGurel, Zaferen_US
dc.contributor.authorHsu, Stephenen_US
dc.contributor.authorSheibani, Naderen_US
dc.date.accessioned2012-10-26T16:40:54Z-
dc.date.available2012-10-26T16:40:54Z-
dc.date.issued2012-07-14en_US
dc.identifier.citationMol Vis. 2012 Jul 14; 18:1895-1906en_US
dc.identifier.issn1090-0535en_US
dc.identifier.pmid22876114en_US
dc.identifier.urihttp://hdl.handle.net/10675.2/724-
dc.description.abstractPurpose: The purpose of this study was to evaluate caspase-14 expression in the retina under normal and diabetic conditions, and to determine whether caspase-14 contributes to retinal microvascular cell death under high glucose conditions.en_US
dc.description.abstractMethods: Quantitative real-time polymerase chain reaction and western blot analysis were used to evaluate caspase-14 expression in retinal cells, including pericytes (PCs), endothelial cells (ECs), astrocytes (ACs), choroidal ECs, and retinal pigment epithelium (RPE) cells. We also determined caspase-14 expression in the retinas of human subjects with or without diabetic retinopathy (DR) and in experimental diabetic mice. Retinal ECs and PCs were infected with adenoviruses expressing human caspase-14 or green fluorescent protein. Caspase-14 expression was also assessed in retinal vascular cells cultured under high glucose conditions. The number of apoptotic cells was determined with terminal deoxynucleotidyl transferase dUTP nick end labeling staining and confirmed by determining the levels of cleaved poly (ADP-ribose) polymerase-1 and caspase-3.en_US
dc.description.abstractResults: Our experiments demonstrated that retinal ECs, PCs, ACs, choroidal ECs, and RPE cells expressed caspase-14, and DR was associated with upregulation and/or activation of caspase-14 particularly in retinal vasculature. High glucose induced marked elevation of the caspase-14 level in retinal vascular cells. There was a significant increase in the apoptosis rate and the levels of cleaved poly (ADP-ribose) polymerase-1 and caspase-3 in retinal ECs and PCs overexpressing caspase-14.en_US
dc.description.abstractConclusions: Our findings indicate that caspase-14 might play a significant role in the pathogenesis of DR by accelerating retinal PC and EC death. Further investigations are required to elaborate the underlying mechanisms.en_US
dc.rightsCopyright © 2012 Molecular Vision.en_US
dc.subjectResearch Articleen_US
dc.titleCaspase-14: A novel caspase in the retina with a potential role in diabetic retinopathyen_US
dc.typeArticleen_US
dc.identifier.pmcidPMC3413417en_US
dc.contributor.corporatenameDepartment of Oral Biology-
dc.contributor.corporatenameDepartment of Ophthalmology-
dc.contributor.corporatenameVision Discovery Institute-
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