Human Platelet-Rich Plasma- and Extracellular Matrix-Derived Peptides Promote Impaired Cutaneous Wound Healing In Vivo

Hdl Handle:
http://hdl.handle.net/10675.2/696
Title:
Human Platelet-Rich Plasma- and Extracellular Matrix-Derived Peptides Promote Impaired Cutaneous Wound Healing In Vivo
Authors:
Demidova-Rice, Tatiana N.; Wolf, Lindsey; Deckenback, Jeffry; Hamblin, Michael R. ( 0000-0001-6431-4605 ) ; Herman, Ira M.
Abstract:
Previous work in our laboratory has described several pro-angiogenic short peptides derived from endothelial extracellular matrices degraded by bacterial collagenase. Here we tested whether these peptides could stimulate wound healing in vivo. Our experiments demonstrated that a peptide created as combination of fragments of tenascin X and fibrillin 1 (comb1) applied into cranial dermal wounds created in mice treated with cyclophosphamide to impair wound healing, can improve the rate of wound closure. Furthermore, we identify and characterize a novel peptide (UN3) created and modified from two naturally-occurring peptides, which are present in human platelet-rich plasma. In vitro testing of UN3 demonstrates that it causes a 50% increase in endothelial proliferation, 250% increase in angiogenic response and a tripling of epithelial cell migration in response to injury.
Editors:
McNeil, Paul L.
Citation:
PLoS One. 2012 Feb 23; 7(2):e32146
Issue Date:
23-Feb-2012
URI:
http://hdl.handle.net/10675.2/696
DOI:
10.1371/journal.pone.0032146
PubMed ID:
22384158
PubMed Central ID:
PMC3285658
Type:
Article
ISSN:
1932-6203
Appears in Collections:
Department of Cellular Biology and Anatomy Faculty Papers

Full metadata record

DC FieldValue Language
dc.contributor.authorDemidova-Rice, Tatiana N.en_US
dc.contributor.authorWolf, Lindseyen_US
dc.contributor.authorDeckenback, Jeffryen_US
dc.contributor.authorHamblin, Michael R.en_US
dc.contributor.authorHerman, Ira M.en_US
dc.contributor.editorMcNeil, Paul L.-
dc.date.accessioned2012-10-26T16:29:36Z-
dc.date.available2012-10-26T16:29:36Z-
dc.date.issued2012-02-23en_US
dc.identifier.citationPLoS One. 2012 Feb 23; 7(2):e32146en_US
dc.identifier.issn1932-6203en_US
dc.identifier.pmid22384158en_US
dc.identifier.doi10.1371/journal.pone.0032146en_US
dc.identifier.urihttp://hdl.handle.net/10675.2/696-
dc.description.abstractPrevious work in our laboratory has described several pro-angiogenic short peptides derived from endothelial extracellular matrices degraded by bacterial collagenase. Here we tested whether these peptides could stimulate wound healing in vivo. Our experiments demonstrated that a peptide created as combination of fragments of tenascin X and fibrillin 1 (comb1) applied into cranial dermal wounds created in mice treated with cyclophosphamide to impair wound healing, can improve the rate of wound closure. Furthermore, we identify and characterize a novel peptide (UN3) created and modified from two naturally-occurring peptides, which are present in human platelet-rich plasma. In vitro testing of UN3 demonstrates that it causes a 50% increase in endothelial proliferation, 250% increase in angiogenic response and a tripling of epithelial cell migration in response to injury.en_US
dc.rightsDemidova-Rice et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.en_US
dc.subjectResearch Articleen_US
dc.subjectBiologyen_US
dc.subjectBiochemistryen_US
dc.subjectBiophysicsen_US
dc.subjectNucleic Acidsen_US
dc.subjectDevelopmental Biologyen_US
dc.subjectMorphogenesisen_US
dc.subjectHistologyen_US
dc.subjectModel Organismsen_US
dc.subjectAnimal Modelsen_US
dc.subjectMolecular Cell Biologyen_US
dc.subjectCellular Typesen_US
dc.subjectProteomicsen_US
dc.subjectMedicineen_US
dc.subjectDermatologyen_US
dc.subjectDrugs and Devicesen_US
dc.subject.meshAnimalsen_US
dc.subject.meshBlood Plateletsen_US
dc.subject.meshCattleen_US
dc.subject.meshCell Movementen_US
dc.subject.meshCell Proliferationen_US
dc.subject.meshCells, Cultureden_US
dc.subject.meshChromatography, Gelen_US
dc.subject.meshChromatography, Ion Exchangeen_US
dc.subject.meshCyclophosphamideen_US
dc.subject.meshEndothelial Cellsen_US
dc.subject.meshExtracellular Matrixen_US
dc.subject.meshHumansen_US
dc.subject.meshKeratinocytesen_US
dc.subject.meshMicrofilament Proteinsen_US
dc.subject.meshNeovascularization, Pathologicen_US
dc.subject.meshPeptidesen_US
dc.subject.meshPlatelet-Rich Plasmaen_US
dc.subject.meshProtein Synthesis Inhibitorsen_US
dc.subject.meshSkinen_US
dc.subject.meshTenascinen_US
dc.subject.meshWound Healingen_US
dc.titleHuman Platelet-Rich Plasma- and Extracellular Matrix-Derived Peptides Promote Impaired Cutaneous Wound Healing In Vivoen_US
dc.typeArticleen_US
dc.identifier.pmcidPMC3285658en_US
dc.contributor.corporatenameDepartment of Cellular Biology and Anatomy-
dc.contributor.corporatenameCollege of Graduate Studies-

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