Intercellular Communication by Exchange of Cytoplasmic Material via Tunneling Nano-Tube Like Structures in Primary Human Renal Epithelial Cells

Hdl Handle:
http://hdl.handle.net/10675.2/660
Title:
Intercellular Communication by Exchange of Cytoplasmic Material via Tunneling Nano-Tube Like Structures in Primary Human Renal Epithelial Cells
Authors:
Domhan, Sophie; Ma, Lili; Tai, Albert; Anaya, Zachary; Beheshti, Afshin ( 0000-0003-4643-531X ) ; Zeier, Martin; Hlatky, Lynn; Abdollahi, Amir
Abstract:
Transfer of cellular material via tunneling nanotubes (TNT) was recently discovered as a novel mechanism for intercellular communication. The role of intercellular exchange in communication of renal epithelium is not known. Here we report extensive spontaneous intercellular exchange of cargo vesicles and organelles between primary human proximal tubular epithelial cells (RPTEC). Cells were labeled with two different quantum dot nanocrystals (Qtracker 605 or 525) and intercellular exchange was quantified by high-throughput fluorescence imaging and FACS analysis. In co-culture, a substantial fraction of cells (67.5%) contained both dyes indicating high levels of spontaneous intercellular exchange in RPTEC. The double positive cells could be divided into three categories based on the preponderance of 605 Qtracker (46.30%), 525 Qtracker (48.3%) and approximately equal content of both Qtrackers (4.57%). The transfer of mitochondria between RPTECs was also detected using an organelle specific dye. Inhibition of TNT genesis by actin polymerization inhibitor (Latrunculin B) markedly reduced intercellular exchange (>60%) suggesting that intercellular exchange in RPTEC was in part mediated via TNT-like structures. In contrast, induction of cellular stress by Zeocin treatment increased tube-genesis in RPTEC. Our data indicates an unexpected dynamic of intercellular communication between RPTEC by exchange of cytosolic material, which may play an important role in renal physiology.
Editors:
McNeil, Paul L.
Citation:
PLoS One. 2011 Jun 27; 6(6):e21283
Issue Date:
27-Jun-2011
URI:
http://hdl.handle.net/10675.2/660
DOI:
10.1371/journal.pone.0021283
PubMed ID:
21738629
PubMed Central ID:
PMC3124493
Type:
Article
ISSN:
1932-6203
Appears in Collections:
Department of Cellular Biology and Anatomy Faculty Papers

Full metadata record

DC FieldValue Language
dc.contributor.authorDomhan, Sophieen_US
dc.contributor.authorMa, Lilien_US
dc.contributor.authorTai, Alberten_US
dc.contributor.authorAnaya, Zacharyen_US
dc.contributor.authorBeheshti, Afshinen_US
dc.contributor.authorZeier, Martinen_US
dc.contributor.authorHlatky, Lynnen_US
dc.contributor.authorAbdollahi, Amiren_US
dc.contributor.editorMcNeil, Paul L.-
dc.date.accessioned2012-10-26T16:27:00Z-
dc.date.available2012-10-26T16:27:00Z-
dc.date.issued2011-06-27en_US
dc.identifier.citationPLoS One. 2011 Jun 27; 6(6):e21283en_US
dc.identifier.issn1932-6203en_US
dc.identifier.pmid21738629en_US
dc.identifier.doi10.1371/journal.pone.0021283en_US
dc.identifier.urihttp://hdl.handle.net/10675.2/660-
dc.description.abstractTransfer of cellular material via tunneling nanotubes (TNT) was recently discovered as a novel mechanism for intercellular communication. The role of intercellular exchange in communication of renal epithelium is not known. Here we report extensive spontaneous intercellular exchange of cargo vesicles and organelles between primary human proximal tubular epithelial cells (RPTEC). Cells were labeled with two different quantum dot nanocrystals (Qtracker 605 or 525) and intercellular exchange was quantified by high-throughput fluorescence imaging and FACS analysis. In co-culture, a substantial fraction of cells (67.5%) contained both dyes indicating high levels of spontaneous intercellular exchange in RPTEC. The double positive cells could be divided into three categories based on the preponderance of 605 Qtracker (46.30%), 525 Qtracker (48.3%) and approximately equal content of both Qtrackers (4.57%). The transfer of mitochondria between RPTECs was also detected using an organelle specific dye. Inhibition of TNT genesis by actin polymerization inhibitor (Latrunculin B) markedly reduced intercellular exchange (>60%) suggesting that intercellular exchange in RPTEC was in part mediated via TNT-like structures. In contrast, induction of cellular stress by Zeocin treatment increased tube-genesis in RPTEC. Our data indicates an unexpected dynamic of intercellular communication between RPTEC by exchange of cytosolic material, which may play an important role in renal physiology.en_US
dc.rightsDomhan et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.en_US
dc.subjectResearch Articleen_US
dc.subjectBiologyen_US
dc.subjectMolecular Cell Biologyen_US
dc.subjectCellular Stress Responsesen_US
dc.subjectCellular Structuresen_US
dc.subjectMedicineen_US
dc.subjectNephrologyen_US
dc.titleIntercellular Communication by Exchange of Cytoplasmic Material via Tunneling Nano-Tube Like Structures in Primary Human Renal Epithelial Cellsen_US
dc.typeArticleen_US
dc.identifier.pmcidPMC3124493en_US
dc.contributor.corporatenameDepartment of Cellular Biology and Anatomy-
dc.contributor.corporatenameCollege of Graduate Studies-

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