Effects of Vitamin D Receptor Knockout, Vitamin D Deficiency, and Diabetes on Corneal Epithelial Nerve Density

Hdl Handle:
http://hdl.handle.net/10675.2/622074
Title:
Effects of Vitamin D Receptor Knockout, Vitamin D Deficiency, and Diabetes on Corneal Epithelial Nerve Density
Authors:
Vick, Sarah
Abstract:
This project is designed to test the hypothesis that vitamin D deficiency exacerbates preexisting primary corneal pathologies. Our lab has established that the corneal epithelium in diabetic mice heals at a faster rate than the epithelium in diabetic vitamin D receptor (VDR) knockout (KO) mice. It is known that within diabetic mice, the corneal nerve density is decreased, and it has been hypothesized that the decreased nerve density can slow corneal epithelium healing within mice. However, it is unknown how VDR KO or vitamin D deficiency in diabetic mice will affect corneal nerve density. In order to determine if nerve density is affected by VDR KO or vitamin D deficiency, mouse corneas were collected, nerves were immuno-stained for confocal microscope photography, and images were analyzed by image processing to determine nerve density. The results demonstrate that in otherwise healthy vitamin D deficient and VDR KO mice lacking the diabetic condition, nerve density was not affected by either vitamin D condition. Corneal nerve density was significantly decreased when vitamin D deficiency or VDR KO was combined with diabetes, confirming the hypothesis that vitamin D deficiency does worsen preexisting corneal pathologies. In addition, this finding may provide an explanation as to why diabetic VDR KO mice have delayed epithelial wound healing compared to control diabetic mice.
Publisher:
Augusta University
Issue Date:
Dec-2018
URI:
http://hdl.handle.net/10675.2/622074
Type:
Thesis
Language:
en_US
Description:
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Series/Report no.:
Fall 2018
Appears in Collections:
Honors Program Theses

Full metadata record

DC FieldValue Language
dc.contributor.authorVick, Sarahen
dc.date.accessioned2019-02-11T14:21:07Z-
dc.date.available2019-02-11T14:21:07Z-
dc.date.issued2018-12-
dc.identifier.urihttp://hdl.handle.net/10675.2/622074-
dc.descriptionThe file you are attempting to access is currently restricted to Augusta University. Please log in with your NetID if off campus.en
dc.description.abstractThis project is designed to test the hypothesis that vitamin D deficiency exacerbates preexisting primary corneal pathologies. Our lab has established that the corneal epithelium in diabetic mice heals at a faster rate than the epithelium in diabetic vitamin D receptor (VDR) knockout (KO) mice. It is known that within diabetic mice, the corneal nerve density is decreased, and it has been hypothesized that the decreased nerve density can slow corneal epithelium healing within mice. However, it is unknown how VDR KO or vitamin D deficiency in diabetic mice will affect corneal nerve density. In order to determine if nerve density is affected by VDR KO or vitamin D deficiency, mouse corneas were collected, nerves were immuno-stained for confocal microscope photography, and images were analyzed by image processing to determine nerve density. The results demonstrate that in otherwise healthy vitamin D deficient and VDR KO mice lacking the diabetic condition, nerve density was not affected by either vitamin D condition. Corneal nerve density was significantly decreased when vitamin D deficiency or VDR KO was combined with diabetes, confirming the hypothesis that vitamin D deficiency does worsen preexisting corneal pathologies. In addition, this finding may provide an explanation as to why diabetic VDR KO mice have delayed epithelial wound healing compared to control diabetic mice.en
dc.language.isoen_USen
dc.publisherAugusta Universityen
dc.relation.ispartofseriesFall 2018en
dc.rightsCopyright protected. Unauthorized reproduction or use beyond the exceptions granted by the Fair Use clause of U.S. Copyright law may violate federal law.en
dc.titleEffects of Vitamin D Receptor Knockout, Vitamin D Deficiency, and Diabetes on Corneal Epithelial Nerve Densityen_US
dc.typeThesisen
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